Retraction: Polyphosphate-induced matrix metalloproteinase-3-mediated proliferation in rat dental pulp fibroblast-like cells is mediated by a Wnt5 signaling cascade

Ozeki, Nobuaki; Yamaguchi, Hideyuki; Hase, Naoko; Hiyama, Taiki; Kawai, Rie; Kondo, Ayami; Nakata, Kohei; Mogi, Makio

doi:10.5582/bst.2015.01041

Cited by 6 publications

(11 citation statements)

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“…We have previously demonstrated that the inflammatory cytokine IL-1β or a cytokine mixture induces MMP-3-regulated cell proliferation and suppresses apoptosis in rat DPFCs (38,39). Moreover, we previously reported that Poly(P)-induced, MMP-3-mediated proliferation in rat DPFCs is mediated by a Wnt5 signaling cascade (10). Considering the effect of MMP-13 on osteogenic cell differentiation, the present findings suggest that targeting the MMP-13 gene in these osteogenic cells may have utility in the treatment of periradicular lesions.…”

Section: Discussionmentioning

confidence: 99%

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Retraction：Polyphosphate-induced matrix metalloproteinase-13 is required for osteoblast-like cell differentiation in human adipose tissue derived mesenchymal stem cells

Ozeki

Mogi

Hase

et al. 2016

BST

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Section: Discussionmentioning

confidence: 99%

“…We previously analyzed the effect of Poly(P) on cell proliferation of rat DPFCs, and found that 0.2 mM Poly(P) is an optimal concentration to enhance the cell growth, whereas more or less than 0.2 mM Poly(P) results in potent inhibition of cell proliferation (10).…”

Section: Poly(p) Induces Osteogenic Differentiation Of Hatmscsmentioning

confidence: 99%

Retraction：Polyphosphate-induced matrix metalloproteinase-13 is required for osteoblast-like cell differentiation in human adipose tissue derived mesenchymal stem cells

Ozeki

Mogi

Hase

et al. 2016

BST

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“…DPFCs were isolated from rat incisors and cultured using a previously described protocol (20). The proportion of plateletderived growth factor receptor (PDGFR)-α positive cells in the total fibroblast-like cell population is a measure of the purity of DPFCs (24). Therefore, using flow cytometry, we evaluated the ratio of PDGFR-α-positive cells as a percentage of the total differentiated cells to determine the purity of the differentiated cell population.…”

Section: Cell Culturementioning

confidence: 99%

“…We previously analyzed the effect of Poly(P) on cell proliferation of DPFCs, and found that 0.1 mM Poly(P) is an optimal concentration to enhance the cell growth, whereas > 0.2 mM Poly(P) results in potent inhibition of cell proliferation (24).…”

Section: Poly(p) Induces Osteogenic Differentiation Of Dpfcsmentioning

confidence: 99%

“…We previously reported that Poly(P) induces MMP-3-mediated proliferation of odontoblast-like cells derived from induced pluripotent stem cells (23). Although we previously reported that Poly(P)-induced MMP-3-mediated proliferation of rat DPFCs is mediated by a Wnt5 signaling cascade (24), it is unknown whether Poly(P) is associated with differentiation. Therefore, we investigated the physiological effect of Poly(P) on DPFC differentiation in vitro.…”

Section: Introductionmentioning

confidence: 99%

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Retraction：Polyphosphate-induced matrix metalloproteinase-3-mediated differentiation in rat dental pulp fibroblast-like cells

Hiyama

Ozeki

Hase

et al. 2015

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Inorganic polyphosphates enhances nucleus pulposus tissue formation in vitro

Gawri

Shiba

Pilliar

et al. 2016

Journal Orthopaedic Research

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Disc degeneration is associated with low back pain for which currently there is no optimal therapy so there is a great need to identify new treatment approaches. Inorganic polyphosphates (polyP) are linear polymers of orthophosphate units varying in chain length and present in many cell types. As polyP has anabolic effects on chondrocytes, we hypothesized that polyP treatment would enhance matrix accumulation by nucleus pulposus (NP) cells. NP cells isolated from bovine caudal discs were grown in 3D culture under normoxic or in select experiments under hypoxic conditions, in the presence or absence of various concentrations and sizes of polyP. Gene expression was determined using RT-PCR. Matrix accumulation was quantified by measuring proteoglycan and collagen contents. DAPI fluorescence shift was used to stain for polyP in tissue. DAPI staining showed polyP present predominantly in the pericellular region of in vitro formed tissue. PolyP treatment enhanced matrix accumulation in a concentration and chain length dependant manner. NP cells exposed to polyP-22 (22 phosphate units length) showed an increase in gene expression of aggrecan, Collagen II, Sox 9, and MMP-13 which was maintained for the 14 days of culture. This suggests that polyP may enhance NP tissue formation in vitro by upregulating the expression of matrix genes. As polyP enhances proteoglycan accumulation even under hypoxic conditions, this raises the possibility that polyP may be a novel treatment to induce NP regeneration. ß

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Retraction: Polyphosphate-induced matrix metalloproteinase-3-mediated proliferation in rat dental pulp fibroblast-like cells is mediated by a Wnt5 signaling cascade

Cited by 6 publications

References 50 publications

Retraction：Polyphosphate-induced matrix metalloproteinase-13 is required for osteoblast-like cell differentiation in human adipose tissue derived mesenchymal stem cells

Retraction：Polyphosphate-induced matrix metalloproteinase-13 is required for osteoblast-like cell differentiation in human adipose tissue derived mesenchymal stem cells

Retraction：Polyphosphate-induced matrix metalloproteinase-3-mediated differentiation in rat dental pulp fibroblast-like cells

Inorganic polyphosphates enhances nucleus pulposus tissue formation in vitro

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