Retrieval and cryopreservation of sperm in spermatophores from cadaveric Indian white shrimp, Fenneropenaeus indicus (H. Milne Edwards, 1837)

Selvakumar, N.; Krishnamoorthy, Dhanasekar; Buelah, Carmel D.; Munuswamy, Natesan

doi:10.1016/j.anireprosci.2018.03.008

Cited by 10 publications

(2 citation statements)

References 31 publications

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“…[75][76][77][78][79][80] For sperm viability in decapod crustacean, spermatozoa are typically stained with conventional (Trypan blue or eosin-nigrosin) or fluorescent (propidium iodide, PI) exclusion dyes in Ca 2+ -free saline (pH 7.4) to examine the integrity of the cell's plasma membrane to determine the proportion of live (unstained) vs. dead (stained) spermatozoa. 22,52,73,[81][82][83] The presence of calcium in semen extenders can trigger the acrosome reaction. [84][85][86] Thus, Ca 2+ -free saline is generally used as the semen extender in most sperm viability studies because the absence of calcium prevents the acrosome reaction and subsequent rupture of the sperm plasma membrane.…”

Section: Sperm Number Morphology and Membrane Integritymentioning

confidence: 99%

“…Given the limitations mentioned above, alternative approaches have been considered such as evaluating the integrity of the plasma membrane to assess sperm viability 75–80 . For sperm viability in decapod crustacean, spermatozoa are typically stained with conventional (Trypan blue or eosin‐nigrosin) or fluorescent (propidium iodide, PI) exclusion dyes in Ca 2+ ‐ free saline (pH 7.4) to examine the integrity of the cell's plasma membrane to determine the proportion of live (unstained) vs. dead (stained) spermatozoa 22,52,73,81–83 84–86 .…”

Section: Collection and Evaluation Of Sperm Quality In Decapod Crusta...mentioning

confidence: 99%

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Recent developments in male fertility evaluation, sperm cryopreservation and artificial fertilisation, and their potential application to decapod crustacean aquaculture

Aquino

Elliott²,

Zeng

et al. 2021

Reviews in Aquaculture

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To maximise productivity, a better understanding of the underlying causes of subfertility that lead to inferior offspring and high mortality is imperative. In decapod crustaceans, most research has focused on female reproductive performance, with little attention given to male fertility. Paternal genetic contribution is critical to both successful embryonic and post‐embryonic development. Assessment of sperm quality can be a direct method to determine male subfertility in decapods. Sperm quality parameters such as sperm concentration and morphology have traditionally been used to determine male reproductive performance, but these procedures are time‐consuming and can only assess a limited number of sperm cells and males. Alternative diagnostic biomarkers used widely in humans and other mammals could be adapted to decapod crustaceans and may be more indicative of sperm fertilisation competence and male reproductive performance. These predictive biomarkers use fluorescent cellular dyes and high‐throughput flow cytometry or computer‐assisted sperm microscopic analysis to evaluate sperm viability, mitochondrial function, acrosome reaction and DNA fragmentation. This review examines current and advanced biomarkers to evaluate sperm quality and further explores state‐of‐the‐art procedures of sperm cryopreservation (conventional vs. vitrification techniques) and artificial fertilisation in decapod crustaceans. Sperm freezing coupled with artificial fertilisation in decapods permits the long‐term storage, controlled timing and selection of individuals for reproduction. Collectively, these tools can be applied to commercial broodstock management to improve productivity and accelerate selective breeding in the crustacean aquaculture industry.

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