Retrospective Analysis of the SARS-CoV-2 Infection Profile in COVID-19 Positive Patients in Vitoria da Conquista, Northeast Brazil

Dantas, Anna Carolina Saúde; Oliveira, Hellenn Cardoso; Gomes, Camila Pacheco; Alves, Daniele L.; Infante, Priscilla D. B.; Caitité, Rosimara de J. A.; Fritsch, Hegger; Cucco, Marina Silveira; Silva, Lucas S. C.; Oliveira, Caline Novais Teixeira; Bittencourt, Rafaela de Souza; Amorim, Aline Teixeira; Nascimento, Ana Luisa P.; Marinho, Francely A. G. C.; Medeiros, Danielle Souto de; Oliveira, Márcio Galvão; Mistro, Sóstenes; Melo, Fabrício Freire de; Pereira, Taiana T. S.; Guimarães, Ana Márcia Sá; Timenetsky, Jorge; Moreira, Pablo Maciel B.; Oliveira, Sandra Helena Penha de; Alcântara, Luíz Carlos Júnior; Giovanetti, Marta; Santos, Luciane Amorim; Fonseca, Vagner; Barreto, Fernanda Khouri; Campos, Guilherme Barreto; Marques, Lucas Miranda

doi:10.3390/v14112424

Cited by 3 publications

(3 citation statements)

References 48 publications

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“…According to the tree clusters, these samples correlate to individuals by place of home or work. Recent data show the spread of SARS-CoV-2 infection in the whole country during pandemics [ 39 , 40 , 41 , 42 , 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of SARS-CoV-2 ORF7a Deletions from COVID-19-Positive Individuals and Its Impact on Virus Spread in Cell Culture

Simas

Costa

Gomes

et al. 2023

Viruses

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The spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causing the COVID-19 outbreak, posed a primary concern of public health worldwide. The most common changes in SARS-CoV-2 are single nucleotide substitutions, also reported insertions and deletions. This work investigates the presence of SARS-CoV-2 ORF7a deletions identified in COVID-19-positive individuals. Sequencing of SARS-CoV-2 complete genomes showed three different ORF7a size deletions (190-nt, 339-nt and 365-nt). Deletions were confirmed through Sanger sequencing. The ORF7a∆190 was detected in a group of five relatives with mild symptoms of COVID-19, and the ORF7a∆339 and ORF7a∆365 in a couple of co-workers. These deletions did not affect subgenomic RNAs (sgRNA) production downstream of ORF7a. Still, fragments associated with sgRNA of genes upstream of ORF7a showed a decrease in size when corresponding to samples with deletions. In silico analysis suggests that the deletions impair protein proper function; however, isolated viruses with partial deletion of ORF7a can replicate in culture cells similarly to wild-type viruses at 24 hpi, but with less infectious particles after 48 hpi. These findings on deleted ORF7a accessory protein gene, contribute to understanding SARS-CoV-2 phenotypes such as replication, immune evasion and evolutionary fitness as well insights into the role of SARS-CoV-2_ORF7a in the mechanism of virus-host interactions.

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Section: Discussionmentioning

confidence: 99%

Evaluation of SARS-CoV-2 ORF7a Deletions from COVID-19-Positive Individuals and Its Impact on Virus Spread in Cell Culture

Simas

Costa

Gomes

et al. 2023

Viruses

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“…1 However, the clinical picture developed by infected individuals remains obscure and concerning due to the high variability of severity even among vaccinated individuals. 2,3…”

Section: Introductionmentioning

confidence: 99%

“…1 However, the clinical picture developed by infected individuals remains obscure and concerning due to the high variability of severity even among vaccinated individuals. 2,3 Influence of polymorphic variations of IFNL, HLA, and IL-6 genes in severe cases of COVID-19…”

Section: Introductionmentioning

confidence: 99%

Influence of polymorphic variations of IFNL, HLA, and IL-6 genes in severe cases of COVID-19

Araújo,

Sgorlon,

Aguiar

et al. 2023

Exp Biol Med (Maywood)

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The administration of vaccination doses to the global population has led to a decrease in the incidence of COVID-19. However, the clinical picture developed by infected individuals remains extremely concerning due to the great variability in the severity of cases even in vaccinated individuals. The clinical progression of the pathology is characterized by various influential factors such as sex, age group, comorbidities, and the genetics of the individual. The immune response to viral infections can be strongly influenced by the genetics of individuals; nucleotide variations called single-nucleotide polymorphisms (SNPs) in structures involved in the innate and adaptive immune response such as interferon (IFN)-λ, human leukocyte antigen (HLA), and interleukin (IL)-6 are frequently associated with pathological progression. In this study, we conducted a review of the main SNPs of these structures that are associated with severity in COVID-19. Searches were conducted on some platforms of the National Center for Biotechnology and Information (NCBI), and 102 studies were selected for full reading according to the inclusion criteria. IFNs showed a strong association with antiviral function, specifically, IFN-λ3 (IL-28B) demonstrated genetic variants commonly related to clinical progression in various pathologies. For COVID-19, rs12979860 and rs1298275 presented frequently described unfavorable genotypes for pathological conditions of hepatitis C and hepatocellular carcinoma. The high genetic variability of HLA was reported in the studies as a crucial factor relevant to the late immune response, mainly due to its ability to recognize antigens, with the HLA-B*46:01 SNP being associated with susceptibility to COVID-19. For IL-6, rs1554606 showed a strong relationship with the clinical progression of COVID-19. In addition, rs2069837 was identified with possible host protection relationships when linked to this infection.

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Clinical-Epidemiological Profile of Dental Professionals Associated with COVID-19 Infection in Southern Peru: A Cross-Sectional Study

Suarez-Cabello

Valdivia

Vergara‐Buenaventura

2022

IJERPH

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Dental professionals have been identified as being at high risk for COVID-19 infection due to close contact with patients and the nature of dental treatments. However, the prevalence of infected dentists in Peru has not been determined. An online electronic survey was sent to dentists registered with the College of Dentists of Arequipa to collect sociodemographic data, medical conditions, and employment characteristics during the COVID-19 pandemic. The clinical characteristics and adverse effects of dentists diagnosed with COVID-19 were also recorded. The overall prevalence of COVID-19 infection was 44%. The highest number of diagnosed patients ranged in age from 31 to 40 years (n = 111; 48.9%). A total of 45.9% of female and 41.6% of male dentists were diagnosed with COVID-19 (p = 0.425). A relationship was found between the district of origin and infection, and dentists working in the public sector during 2021 showed a greater trend of becoming infected (57.6%) (p < 0.05). The antigen swab test was the diagnostic test most frequently used (38%), and cough was the symptom most reported. Only 0.6% reported being hospitalized, 10.6% reported needing oxygen during hospitalization, and 0.6% were admitted to an intensive care unit. As in previous studies in other countries, the rate of COVID-19 infection among Peruvian dentists is high. It is recommended that dentists carry out infection control measures while ministries of health and dental associations take measures to ensure their safety.

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Retrospective Analysis of the SARS-CoV-2 Infection Profile in COVID-19 Positive Patients in Vitoria da Conquista, Northeast Brazil

Cited by 3 publications

References 48 publications

Evaluation of SARS-CoV-2 ORF7a Deletions from COVID-19-Positive Individuals and Its Impact on Virus Spread in Cell Culture

Evaluation of SARS-CoV-2 ORF7a Deletions from COVID-19-Positive Individuals and Its Impact on Virus Spread in Cell Culture

Influence of polymorphic variations of IFNL, HLA, and IL-6 genes in severe cases of COVID-19

Clinical-Epidemiological Profile of Dental Professionals Associated with COVID-19 Infection in Southern Peru: A Cross-Sectional Study

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