Retrospective study of adenovirus in autopsied pulmonary tissue of pediatric fatal pneumonia in South China

Ou, Zhiying; Zeng, Qing; Wang, Fenghua; Xia, Huimin; Lu, Jun-Peng; Xià, Jì; Gong, Sitang; Deng, Li; Zhang, Jiantao; Zhou, Rong

doi:10.1186/1471-2334-8-122

Cited by 35 publications

(46 citation statements)

References 16 publications

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“…Our data underscore the important role of HAdV in adult ARTIs in China. The 0.8% detection rate of HAdV in this study is lower than the 2.0 to 9.14% reported in several previously published studies [12,22,23]. This disparity cannot be attributed to the detection method used, as our PCR experiments which target a different hexon gene fragment and a different gene (E1A) are consistent with our PCR screening results.…”

Section: Discussionsupporting

confidence: 46%

“…This disparity cannot be attributed to the detection method used, as our PCR experiments which target a different hexon gene fragment and a different gene (E1A) are consistent with our PCR screening results. This disparity may be attributed to: (1) differences in the age of the study population-the subjects recruited in this study were adults, whereas those in other studies were mainly children [12,22,23]. Childhood exposure to some adenovirus serotypes may generate protective immunity in response to the same serotypes, which could lead to the low infection rates observed in adults; (2) the method of sample collection-adenoviruses were detected from nasal and throat swabs of upper respiratory tract infections in this study, whereas those in other studies were recovered from nasal aspirate [23] or autopsied pulmonary tissue of pediatric fatal pneumonia [12]; (3) the impact of geographical location.…”

Section: Discussionmentioning

confidence: 56%

“…However, incidences of adenovirus-associated illnesses are higher among children than among adults. As such, in China, the impact of HAdVs on respiratory infection is well characterized in children [10][11][12][13][14][15] but not in adults.…”

Section: Introductionmentioning

confidence: 99%

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Detection of three human adenovirus species in adults with acute respiratory infection in China

Guo

Gonzalez

Zhou

et al. 2011

Eur J Clin Microbiol Infect Dis

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Human adenoviruses (HAdVs) are recognized as causal agents in a wide range of human diseases. However, researchers lack sufficient data on the exact HAdV species and serotypes associated with adult acute respiratory tract infections (ARTIs). To detect and characterize HAdV infections in adults in China, clinical specimens were collected from 10,310 adults with ARTIs from May 2005 to July 2010. The partial HAdV hexon gene was amplified by polymerase chain reaction (PCR), sequenced, and phylogenetically analyzed. HAdVs were detected in 86 samples (0.8%), of which 67 (77.9%) were species B (HAdV-3, -7, -11, and -14), 7 (8.1%) were species C (HAdV-1, -2, and -6), and 12 (14%) were species E (HAdV-4). HAdV-3 was the most frequently detected serotype (41/86, 47.7%), followed by HAdV-7 (13/86, 15.1%), HAdV-4 (12/86, 14.0%), and HAdV-11 (11/86, 12.8%). Patients 14-25 years old (60.5%) exhibited a higher rate of adenovirus detection than older patients. Co-infections with other respiratory viruses were observed in samples positive for HAdV species B and E. Human rhinovirus was the most commonly found virus in patients with HAdV infection. These findings provide baseline data for the surveillance and control of HAdV infection in China.

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Section: Discussionsupporting

confidence: 46%

Section: Discussionmentioning

confidence: 56%

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Detection of three human adenovirus species in adults with acute respiratory infection in China

Guo

Gonzalez

Zhou

et al. 2011

Eur J Clin Microbiol Infect Dis

View full text Add to dashboard Cite

show abstract

“…Although these serotypes appear to have low prevalence in our area, severe clinical disease and even fatal outcome were reported with HAdV serotypes 4, 11, 14 and 41 worldwide. 8,9 HAdV-14 has recently emerged and circulated in the USA since 2003 as a virulent strain causing acute respiratory disease. Recently, an outbreak of HAdV-14-associated pneumonia in 21 patients was reported in Southeast Alaska.…”

Section: Variablesmentioning

confidence: 99%

“…Three HAdV serotypes, 3, 4 and 41, were identified by sequencing a region of the hexon gene. 9 Recently, a sudden death of a child from myocarditis was reported due to an uncontrolled inflammatory response to a disseminated adenovirus serotype 3 infection in the UK. 10 Sequences of hypervariable regions of hexon gene provide excellent correlation for identification of adenoviruses at the serotype level.…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology and clinical presentation of human adenovirus infections in Kansas City children

Selvaraju

Kovac

Dickson

et al. 2011

Journal of Clinical Virology

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Design and application of a real-time polymerase chain for the detection and subsequent characterization of respiratory adenoviral infections

Lee

et al. 2014

J. Med. Virol.

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Human adenoviruses (HADVs) comprise at least 54 types and cause a wide spectrum of respiratory tract infections; early diagnosis and epidemiological monitoring of HADV infections requires a rapid and sensitive assay. The use of a real-time polymerase chain reaction (PCR) assay was evaluated with one set of in-house designed primers for respiratory adenoviral infections. The assay was first validated by detecting successfully 6 representative types and 100 clinical isolates. A concomitant prospective surveillance of viral aetiology using conventional cultures and PCR assays in 160 febrile children with acute respiratory tract symptoms was conducted between May 2010 and July 2011. Viral aetiologies were confirmed in 72 (45%) cases using conventional cultures, including 51 adenoviral infections. The concordance between the real-time PCR and culture was good (Kappa = 0.94), and two additional culture-negative adenovirus infections were identified. During the study period (January 2011), an adenoviral community epidemic occurred. Adenovirus B3 was the predominant type in this epidemic (69.8%), followed by C2 (5.7%), C1 (5.7%), C5 (1.9%), E4 (1.9%), C6 (1.9%), F41 (1.9%), and 4 unclassified species C (7.5%). Significantly prolonged duration of fever (>5 days), higher leukocyte counts, higher neutrophil counts, and higher C-reactive protein levels were in the adenoviral infected group (n = 53, P < 0.001), compared with the non-adenoviral infected group (n = 107). In conclusion, this in-house real-time PCR is capable of detecting adenoviral respiratory infections of various types in children; and patients with adenoviral aetiology suffered from more severe clinical manifestations.

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Retrospective study of adenovirus in autopsied pulmonary tissue of pediatric fatal pneumonia in South China

Cited by 35 publications

References 16 publications

Detection of three human adenovirus species in adults with acute respiratory infection in China

Detection of three human adenovirus species in adults with acute respiratory infection in China

Molecular epidemiology and clinical presentation of human adenovirus infections in Kansas City children

Design and application of a real-time polymerase chain for the detection and subsequent characterization of respiratory adenoviral infections

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