Reumatología Pediátrica. Breve historia y situación de la especialidad en España

Carvajal, Liliana López; Hoyos, Laura Catalina Mazo; Arias, Jaiberth Antonio Cardona

doi:10.3823/1307

Cited by 3 publications

(2 citation statements)

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“…Leishmaniasis is one of the seven main tropical diseases and continues to be one of the major public health problems around the world (Torres-Guerrero et al, 2017). Re-evaluation of treatment guidelines for CL is urgently needed because there is high risk of toxicity of pentavalent antimony and treatment failure is increasing (Von Stebut, 2015; López-Carvajal, Mazo & Cardona-Arias, 2016).…”

Section: Discussionmentioning

confidence: 99%

Label-free quantitative proteomic analysis reveals potential biomarkers for early healing in cutaneous leishmaniasis

Montoya

López

Vélez

et al. 2019

PeerJ

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BackgroundLeishmaniasis is a parasitic disease caused by more than 20 species of the Leishmania genus. The disease is globally distributed and is endemic in 97 countries and three territories in the tropical and subtropical regions. The efficacy of the current treatments is becoming increasingly low either due to incomplete treatment or resistant parasites. Failure of treatment is frequent, and therefore, the search for early biomarkers of therapeutic response in cutaneous leishmaniasis (CL) is urgently needed.ObjectiveThe aim of this study was to compare the proteomic profiles in patients with CL before and after 7 days of treatment and identify early biomarkers of curative response.MethodsFour patients with a parasitological diagnosis of leishmaniasis with confirmation of species by PCR-RFLP were recruited. All patients had a single lesion, and a protein from the middle of the ulcer was quantified by liquid chromatography and mass spectrometry.ResultsA total of 12 proteins showed differential expression in the comparative LC-electrospray ionization MS/MS (LC-ESI-MS/MS) triplicate analysis. Seven of them were up-regulated and five of them were down-regulated. Calcium binding proteins A2, A8, and A9 and hemoglobin subunits alpha-2 and delta showed high correlation with epidermis development and immune response.ConclusionWe identified changes in the profiles of proteins that had a positive therapeutic response to the treatment. The proteins identified with differential expression are related to the reduction of inflammation and increased tissue repair. These proteins can be useful as biomarkers for early monitoring of therapeutic response in CL.

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Section: Discussionmentioning

confidence: 99%

Label-free quantitative proteomic analysis reveals potential biomarkers for early healing in cutaneous leishmaniasis

Montoya

López

Vélez

et al. 2019

PeerJ

View full text Add to dashboard Cite

show abstract

“…Leishmaniasis is one of the seven main tropical diseases and continues to be one of the major public health problems around the world (23). Reevaluation of treatment guidelines for cutaneous leishmaniasis is urgently needed because there is high risk of toxicity of pentavalent antimony and treatment failure is increasing (24,25).…”

Section: Discussionmentioning

confidence: 99%

Peer Review #2 of "Label-free quantitative proteomic analysis reveals potential biomarkers for early healing in cutaneous leishmaniasis (v0.1)"

2019

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Background. Leishmaniasis is a parasitic disease caused by more than 20 species of the Leishmania genus. The disease is globally distributed and is endemic in 97 countries and three territories in the tropical and subtropical regions. The efficacy of the current treatments is becoming increasingly low either due to incomplete treatment or resistant parasites. Failure of treatment is frequent, and therefore, the search for early biomarkers of therapeutic response in cutaneous leishmaniasis (CL) is urgently needed. Objective: The aim of this study was to compare the proteomic profiles in patients with CL before and after 7 days of treatment and identify early biomarkers of curative response. Methods. Four patients with a parasitological diagnosis of leishmaniasis with confirmation of species by PCR-RFLP were recruited. All patients had a single lesion, and a protein from the middle of the ulcer was quantified by liquid chromatography and mass spectrometry (LC-MS). Results. Twelve proteins showed differential expression in the comparative LCelectrospray ionization MS/MS (LC-ESI-MS/MS) triplicate analysis. Seven of them were upregulated and five of them were down-regulated. Calcium binding proteins A2, A8, and A9 and hemoglobin subunits alpha-2 and delta showed high correlation with epidermis development and immune response. Conclusion. We identified changes in the profiles of proteins that had a positive therapeutic response to the treatment. The proteins identified with differential expression are related to the reduction of inflammation and increased tissue repair. These proteins can be useful as biomarkers for early monitoring of therapeutic response in cutaneous leishmaniasis.

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Cutaneous Leishmaniasis

Nassiri-Kashani¹,

Khamesipour²,

Firooz³

2018

Pigmented Ethnic Skin and Imported Dermatoses

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Reumatología Pediátrica. Breve historia y situación de la especialidad en España

Cited by 3 publications

References 9 publications

Label-free quantitative proteomic analysis reveals potential biomarkers for early healing in cutaneous leishmaniasis

Label-free quantitative proteomic analysis reveals potential biomarkers for early healing in cutaneous leishmaniasis

Peer Review #2 of "Label-free quantitative proteomic analysis reveals potential biomarkers for early healing in cutaneous leishmaniasis (v0.1)"

Cutaneous Leishmaniasis

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