Reusable glucose sensing using carbon nanotube-based self-assembly

Abstract

Lipid functionalized single walled carbon nanotube-based self assembly forms a super-micellar structure. This assemblage has been exploited to trap glucose oxidase in a molecular cargo for glucose sensing. The advantage of such a molecular trap is that all components of this unique structure (both the trapping shell and the entrapped enzyme) are reusable and rechargeable. The unique feature of this sensing method lies in the solid state functionalization of single walled carbon nanotubes that facilitates liquid state immobilization of the enzyme. The method can be used for soft-immobilization (a new paradigm in enzyme immobilization) of enzymes with better thermostability that is imparted by the strong hydrophobic environment provided through encapsulation by the nanotubes.

Nanotechnology based activation-immobilization of psychrophilic pectate lyase: A novel approach towards enzyme stabilization and enhanced activity

“…Due to such re-structuring, a pseudo-micellar structure of the lipid-SWNT complex i.e. nanorope formation [8,18] takes place which is readily soluble in water. The self-assembly collapses upon mild sonication and when it is allowed to re-assemble again in presence of…”

“…A c c e p t e d M a n u s c r i p t 11 enzymes [8] which are present in the aqueous solvent or buffer; it entraps enzymes into the inner core of the supramolecular self-assembly (nanorope).…”

“…The SWNT: PL The reuse of SWNT assembly [8,18] as described earlier was used for packing of fresh enzymes or the same enzymes for a number of cycles. Whether other NPs can influence the enzyme activity, NP-PL was packed inside the SWNT cage and SWNT-PL activity was assayed.…”

“…The basic principle of solid state functionalization at nanoscale had first been explored by Bhattacharyya et al [7]. The theoretical insight of lipid functionalized SWNT self-assembly [18] (nanorope design) which serve as a molecular machine has been reported previously by Bhattacharyya et al [8,18] for developing a reusable glucose sensor. This principle has been utilized for developing and studying the thermal stability and re-usability of a cold-tolerant pectate lyase.…”

Enhanced functionality and stabilization of a cold active laccase using nanotechnology based activation-immobilization

“…by Bhattacharyya et al (2012). The theoretical insight of lipid functionalized SWNT self-assembly (nanorope design) which serve as a molecular machine has been reported previously by Bhattacharyya et al (2013) for developing a reusable glucose sensor. This background has been utilized in our studies for developing and studying a SWNT-laccase system with value-added properties of wide thermal stability and re-usability.…”