Revascularization of decellularized lung scaffolds: principles and progress

Stabler, Collin T.; Lecht, Shimon; Mondrinos, Mark J.; Goulart, Ernesto; Lazarovici, Philip; Lelkes, Peter I.

doi:10.1152/ajplung.00237.2015

Cited by 49 publications

(36 citation statements)

References 128 publications

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“…However, the studies reviewed herein also suggest that ECM scaffolds have potential as cell delivery scaffolds, although this significantly increases treatment costs and has a more difficult regulatory pathway as a combination product. More long term development could result in the use of decellularized ECM for whole organ engineering [92–95]; however, this increase in complexity creates numerous more challenges that must be overcome, including creating fully functional constructs and preventing thrombosis in the vasculature [96, 97]. …”

Section: Current Challenges and Future Opportunitiesmentioning

confidence: 99%

Controlling stem cell behavior with decellularized extracellular matrix scaffolds

Agmon

Christman

2016

Current Opinion in Solid State and Materials Science

157

104

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Decellularized tissues have become a common regenerative medicine platform with multiple materials being researched in academic laboratories, tested in animal studies, and used clinically. Ideally, when a tissue is decellularized the native cell niche is maintained with many of the structural and biochemical cues that naturally interact with the cells of that particular tissue. This makes decellularized tissue materials an excellent platform for providing cells with the signals needed to initiate and maintain differentiation into tissue-specific lineages. The extracellular matrix (ECM) that remains after the decellularization process contains the components of a tissue specific microenvironment that is not possible to create synthetically. The ECM of each tissue has a different composition and structure and therefore has unique properties and potential for affecting cell behavior. This review describes the common methods for preparing decellularized tissue materials and the effects that decellularized materials from different tissues have on cell phenotype.

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Section: Current Challenges and Future Opportunitiesmentioning

confidence: 99%

Controlling stem cell behavior with decellularized extracellular matrix scaffolds

Agmon

Christman

2016

Current Opinion in Solid State and Materials Science

157

104

View full text Add to dashboard Cite

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“…This long track record and acceptance by clinicians is indicative of the promise of new ECM-based biomaterials that can be applied to repair or regenerate damaged, missing, or dysfunctional tissues and organs without activating host immune response, [1] or even serve as model materials for in vitro healthy or diseased tissue modeling. [11,12] Despite dECM’s demonstrated advantages as a raw material and promise for use in an extensive range of tissue and organ targets including but not limited to: heart, [13] muscle, [1,14] nerve, [15] liver, [3,16,17] lung, [18,19] kidney, [4,20] bone, [21] skin, [22] bladder, [23] ovary, [24] and penis, [25] the existing processing routes for creating diverse sets of clinically practical, tissue-specific dECM-based biomaterials remain limited.…”

Section: Introductionmentioning

confidence: 99%

“Tissue Papers” from Organ‐Specific Decellularized Extracellular Matrices

Jakus

Laronda

Rashedi

et al. 2017

Adv Funct Materials

109

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Using an innovative, tissue-independent approach to decellularized tissue processing and biomaterial fabrication, the development of a series of “tissue papers” derived from native porcine tissues/organs (heart, kidney, liver, muscle), native bovine tissue/organ (ovary and uterus), and purified bovine Achilles tendon collagen as a control from decellularized extracellular matrix particle ink suspensions cast into molds is described. Each tissue paper type has distinct microstructural characteristics as well as physical and mechanical properties, is capable of absorbing up to 300% of its own weight in liquid, and remains mechanically robust (E = 1–18 MPa) when hydrated; permitting it to be cut, rolled, folded, and sutured, as needed. In vitro characterization with human mesenchymal stem cells reveals that all tissue paper types support cell adhesion, viability, and proliferation over four weeks. Ovarian tissue papers support mouse ovarian follicle adhesion, viability, and health in vitro, as well as support, and maintain the viability and hormonal function of nonhuman primate and human follicle-containing, live ovarian cortical tissues ex vivo for eight weeks postmortem. “Tissue papers” can be further augmented with additional synthetic and natural biomaterials, as well as integrated with recently developed, advanced 3D-printable biomaterials, providing a versatile platform for future multi-biomaterial construct manufacturing.

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“…It seems clear, however, that the differentiation and maturation of cells in the reseeded graft need to be improved. According to Stabler et al (2015), reconstitution of physiological pulmonary vasculature in its entirety will significantly improve the generation of whole lungs through bioengineering organs. Therefore, it could be expected that variations in arterial and alveolar pressures could influence cell adhesion, considering that a reduced flow through the lung circuit would decrease cell distribution.…”

Section: Discussionmentioning

confidence: 99%

Behavior of vascular resistance undergoing various pressure insufflation and perfusion on decellularized lungs

Palma

Nonaka

Campillo

et al. 2016

Journal of Biomechanics

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a b s t r a c tBioengineering of functional lung tissue by using whole lung scaffolds has been proposed as a potential alternative for patients awaiting lung transplant. Previous studies have demonstrated that vascular resistance (Rv) could be altered to optimize the process of obtaining suitable lung scaffolds. Therefore, this work was aimed at determining how lung inflation (tracheal pressure) and perfusion (pulmonary arterial pressure) affect vascular resistance. This study was carried out using the lungs excised from 5 healthy male Sprague-Dawley rats. The trachea was cannulated and connected to a continuous positive airway pressure (CPAP) device to provide a tracheal pressure ranging from 0 to 15 cmH 2 O. The pulmonary artery was cannulated and connected to a controlled perfusion system with continuous pressure (gravimetric level) ranging from 5 to 30 cmH 2 O. Effective Rv was calculated by ratio of pulmonary artery pressure (P PA ) by pulmonary artery flow (V 0 PA ). Rv in the decellularized lungs scaffolds decreased at increasing V 0 PA, stabilizing at a pulmonary arterial pressure greater than 20 cmH 2 O. On the other hand, CPAP had no influence on vascular resistance in the lung scaffolds after being subjected to pulmonary artery pressure of 5 cmH 2 O. In conclusion, compared to positive airway pressure, arterial lung pressure markedly influences the mechanics of vascular resistance in decellularized lungs.

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Revascularization of decellularized lung scaffolds: principles and progress

Abstract: Stabler CT, Lecht S, Mondrinos MJ, Goulart E, Lazarovici P, Lelkes PI. Revascularization of decellularized lung scaffolds: principles and progress.

Cited by 49 publications

References 128 publications

Controlling stem cell behavior with decellularized extracellular matrix scaffolds

Controlling stem cell behavior with decellularized extracellular matrix scaffolds

“Tissue Papers” from Organ‐Specific Decellularized Extracellular Matrices

Behavior of vascular resistance undergoing various pressure insufflation and perfusion on decellularized lungs

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