Revealing floral metabolite network in tuberose that underpins scent volatiles synthesis, storage and emission

Kutty, Nithya N.; Ghissing, Upashana; Mitra, Adinpunya

doi:10.1007/s11103-021-01171-7

Cited by 23 publications

(14 citation statements)

References 73 publications

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“…Among them, 4CL, CCR, and CAD were expressed in each developmental stage of YJ, and all of them were down-regulated in YJC, which were signi cantly negatively correlated with various benzene metabolites. 4CL plays an important role in the synthesis of benzene/phenylpropane compounds, which has also been con rmed in agave, holy basil, and mulberry [35][36][37][38][39]. Zhang et al [38] comprehensively analyzed the endogenous volatile compounds, transcriptome, and enzymatic activities of plum blossoms, which showed that PmCAD1 played an important role in the biosynthesis of cinnamyl alcohol in vitro.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome and metabolome reveal key genes in the phenylpropane pathway to regulate the floral fragrance of Rhododendron fortunei

Yang

Qin

Jia

et al. 2022

Preprint

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Background: Floral aroma is an important plant trait and it is one of the main traits of Rhododendron varieties which affect their ornamental value. The volatile compounds of floral aroma are secondary metabolites. In order to reveal the key genes of the phenylpropane pathway, which regulates the fragrance of Rhododendron fortunei, we performed a comprehensive transcriptome and metabolome analysis of the petals of the petals of two alpine rhododendrons from two different varieties: the scented Rhododendron fortunei and the non-scented Rhododendron ‘Nova Zembla’. Results: Transcriptomic and qRT-PCR results showed that nine candidate genes were highly expressed in R. fortunei, but down-regulated in R. ‘Nova Zembla’. The correlation analysis of candidate genes and metabolites revealed nine key genes involved in the regulation of floral aroma in the phenylpropane pathway. Among them, EGS was significantly positively correlated with various volatile benzene/phenylpropane compounds, and significantly negatively correlated with various non-volatile compounds; CCoAOMT, PAL, C4H,and BALDH were significantly negatively correlated with various volatile benzene/phenylpropane compounds, and significantly positively correlated with various non-volatile compounds. CCR, CAD, 4CL, and EGSwere significantly negatively correlated with various benzene/phenylpropane compounds. The validation of RfSAMT proved that RfSAMT gene regulated the synthesis of aromatic substances in R. fortunei. Conclusion: The findings of this study indicated that key candidate genes and metabolites involved in the biosynthetic pathway of phenylpropane may regulate the fragrance of R. fortunei. This lays a foundation for further research on the molecular mechanism of fragrance synthesis in the genus Rhododendron.

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Section: Discussionmentioning

confidence: 99%

Transcriptome and metabolome reveal key genes in the phenylpropane pathway to regulate the floral fragrance of Rhododendron fortunei

Yang

Qin

Jia

et al. 2022

Preprint

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“…These metabolites are related to energetic sugar metabolism, directly involved in the glycolysis pathway through 2,3-diphosphoglyceric acid, 37 being extended to sorbose and threitol, which are natural sugars. 38,39 Cysteine (log 2 (FC) = -1.42) and the carbohydrates fructose (log 2 (FC) = -1.20) and α-galactose-1-phosphate (log 2 (FC) = -1.02) were present at lower concentration in FIP than in CP, indicating the reduction of cysteine concentration potentially be related to the synthesis of methionine and glutathione. 40 After one day, plants were inoculated with Ts fungus, α-lactose (log 2 (FC) = 1.67) and galactono-1,4-lactone (log 2 (FC) = 1.08) were present at higher concentrations in plant-Ts treatment group.…”

Section: Identification Of Significant Metabolites and Determination ...mentioning

confidence: 99%

Metabolomics Analysis of C. lanceolatum Roots in the Presence of Its Endophytic Fungi

Serrano¹,

Lacerda²,

Moura³

et al. 2023

J. Braz. Chem. Soc.

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The aim of this work was to identify and quantify the metabolites present in the roots of Combretum lanceolatum inoculated with its endophytic fungi. The metabolomics was accomplished using the 1H nuclear magnetic resonance (NMR) spectral data and evaluated via rNMR software and Madison Metabolomics Consortium Database (MMCD). The principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) showed that plants inoculated with Trichoderma spirale (Ts) present differentiation and discrimination over the time as compared to control. Seven days after Ts fungal inoculation, 15 metabolites were identified at different concentrations comparing to the control plants. The plants inoculated with Ts fungus present the metabolites spermidine and pantothenate in higher concentrations and 3-hydroxybutyric acid and β-alanine in lower concentrations as compared to control plants, indicating any response to biotic stress. These metabolites are involved in various plant processes, including secondary metabolites biosynthesis, energy metabolism and self-defense. Therefore, this work demonstrates the diversification of primary metabolites composition influenced by endophytes inoculation.

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“…Relative expression levels of the genes were determined using Power SYBR Green (Thermo Fisher Scientific) on an Applied Biosystems thermocycler equipped with ABI Prism â 7000 Sequence Detection System. Reaction mix and cycling parameters were adjusted as described by Kutty et al (2021). The reaction parameters were: one cycle at 50°C for 2 min, one cycle at 95°C for 5 min, followed by 40 cycles at 94°C for 30 s, 60°C for 30 s and 72°C for 45 s. Relative gene expression levels were calculated using three reference genes (CiActin, CiTubulin and CiGAPDH) following the 2 ÀΔΔCt method (Livak & Schmittgen 2001).…”

Section: Validation Of Rna-seq Data With Quantitative Real-time Pcr (...mentioning

confidence: 99%

Comparative transcriptome analysis reveals an insight into the candidate genes involved in anthocyanin and scent volatiles biosynthesis in colour changing flowers of Combretum indicum

et al. 2022

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Combretum indicum is a widely cultivated ornamental species displaying the distinct phenomenon of floral colour change. Flowers display a gradual colour change from white to red, attributed to increased cyanidin 3-O glucoside in petal tissues. The differently coloured flowers also emanate a complex blend of VOCs with trans-linalool oxide (furanoid) as the major compound in the emission profile.• To understand molecular mechanisms regulating floral colour shifts and scent biosynthesis, we performed Illumina transcriptome sequencing, including de novo assembly and functional annotation, for the two stages of floral maturation (white and red). Homology analysis with functional classification identified 84 and 42 candidate genes associated with pigment and scent biosynthesis, respectively. Genes encoding transcription factors, such as MYB, ERF, WD40, WRKY, NAC, bHLH and bZIP, that play critical roles in regulating specialized metabolism were also identified in the transcriptome data.• Differences in expression of genes were consistent with accumulation patterns of anthocyanins in the two different flower colours. A clear upregulation of flavonoid biosynthesis genes in red flower tissue is associated with increased pigment content. RT-qPCR-based expression analyses gave results consistent with the RNA-Seq data, suggesting the sequencing data are consistent and reliable.• This study presents the first report of genetic information for C. indicum. Gene sequences generated from RNA-Seq, along with candidate genes identified by pathway mapping and their expression profiles, provide a valuable resource for subsequent studies towards molecular understanding of specialized metabolism in C. indicum flowers.

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Revealing floral metabolite network in tuberose that underpins scent volatiles synthesis, storage and emission

Cited by 23 publications

References 73 publications

Transcriptome and metabolome reveal key genes in the phenylpropane pathway to regulate the floral fragrance of Rhododendron fortunei

Transcriptome and metabolome reveal key genes in the phenylpropane pathway to regulate the floral fragrance of Rhododendron fortunei

Metabolomics Analysis of C. lanceolatum Roots in the Presence of Its Endophytic Fungi

Comparative transcriptome analysis reveals an insight into the candidate genes involved in anthocyanin and scent volatiles biosynthesis in colour changing flowers of Combretum indicum

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