Revealing lipid droplets evolution at nanoscale under proteohormone stimulation by a BODIPY- hexylcarbazole derivative

Liu, Xiaolu; Lü, Xin; Zhu, Tong; Du, Wenli; Yang, Zhenle; Cao, Hongzhi; Wang, Siyou; Tian, Yupeng; Zhang, Zhongping; Zhang, Ruilong; Souza, Senio Campos De; Tian, Xiaohe

doi:10.1016/j.bios.2020.112871

Cited by 24 publications

(8 citation statements)

References 43 publications

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“…Fluorescence imaging has become a robust tool to directly image and monitor vital biomolecules and their associated processes. − Various fluorescent probes with blue to near-infrared emissions have been elegantly synthesized for LD dynamic imaging. − However, most of these LD probes suffer from low resolution (>200 nm) . To overcome this bottleneck, super-resolution imaging techniques have become an indispensable approach to accurately visualize nanoscale structures below 200 nm. − Indeed, a few research groups successfully constructed new LD-specific fluorescent probes for super-resolution imaging using structured illumination microscopy (SIM) and stimulated emission depletion (STED) microscopy. − Interesting imaging results of nano-sized cytosolic LDs with improved higher resolution over traditional confocal laser scanning microscopy (CLSM) have been acquired. However, critical imaging parameters, such as fluorescence quantum yield (QY), imaging contrast, and incubation concentration, need further improvement.…”

Section: Introductionmentioning

confidence: 99%

Structure Rigidification Promoted Ultrabright Solvatochromic Fluorescent Probes for Super-Resolution Imaging of Cytosolic and Nuclear Lipid Droplets

et al. 2022

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Lipid droplets (LDs) containing cytosolic and nuclear LDs have recently received increasing attention because of their diverse biological roles in living systems. However, developing fluorescent probes for super-resolution visualization of these subcellular LDs still remains challenging due to insufficient fluorescence brightness and poor nuclear membrane permeability. Herein, we rationally synthesized a series of ultrabright solvatochromic fluorescent probes based on benzoboranils (BBAs) for LD-specific super-resolution imaging using structured illumination microscopy (SIM). The rigidly structured probes exhibit ultrahigh fluorescence quantum yields of up to 99.9% in low-polar solvents. They also show more significant fluorescence enhancements in lipid environments than commercial LD probes. Owing to these excellent merits, our lipophilic fluorescent probes can specifically light up subcellular LDs at ultralow concentrations down to 10 nM. Further use of BBA-CF 3 for super-resolution SIM imaging of cytosolic and nuclear LDs and their fusion process was successfully achieved. The unprecedented spatial resolution for nuclear LDs with an FWHM value of 142 nm was also acquired. Collectively, our ultrabright fluorescent probes hold tremendous potential to unveil the mysterious roles of cytosolic and nuclear LDs in biological research using SIM.

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Section: Introductionmentioning

confidence: 99%

Structure Rigidification Promoted Ultrabright Solvatochromic Fluorescent Probes for Super-Resolution Imaging of Cytosolic and Nuclear Lipid Droplets

et al. 2022

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“…Because of its noninvasiveness, high sensitivity, superior spatiotemporal resolution, and simple operation, the fluorescence imaging technique has become a powerful tool for the real-time and in situ detection of biomolecules at subcellular levels and in vivo . − Until now, although a large number of fluorescent probes have been reported for specific imaging LDs, ,− few are polarity-sensitive. − ,− In addition, most of the polarity-sensitive probes are only limited for dynamic imaging of LDs. − Due to the lack of research on various disease models, the clear elucidation of the intrinsic relationship between diseases and LD polarity has not been really achieved. Currently, the disease model using LD polarity mainly focuses on the diagnosis of cancer at the level of cells or animal tissues/organs. ,,− To our knowledge, the visualization of LD polarity in vivo models, such as inflammation or clinical cancer patient samples, remains underexplored.…”

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confidence: 99%

Lipid Droplet-Specific Fluorescent Probe for In Vivo Visualization of Polarity in Fatty Liver, Inflammation, and Cancer Models

et al. 2021

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Elucidating the intrinsic relationship between diseases and lipid droplet (LD) polarity remains a great challenge owing to the lack of the research on multiple disease models. Until now, the visualization of abnormal LD polarity in models of inflammation and clinical cancer patient samples has not been achieved. To meet the urgent challenge, we facilely synthesized a robust LD-specific and polarity-sensitive fluorescent probe (LD-TTP), which consists of a triphenylamine segment as an electron-donor group (D) and a pyridinium as an electron-acceptor moiety (A), forming a typical D−π−A molecular configuration. Owing to the unique intramolecular charge transfer effect, LD-TTP exhibits high sensitivity to polarity change in the linear range from Δf = 0.258 to 0.312, with over 278-fold fluorescence enhancement. Moreover, we revealed that LD-TTP possessed satisfactory ability for sensitively monitoring LDpolarity changes in living cells. Using LD-TTP, we first demonstrated the detection of LD-polarity changes in fatty liver tissues and inflammatory living mice via confocal laser scanning fluorescence imaging. Surprisingly, the visualization of LD polarity has been achieved not only at the cellular levels and living organs but also in surgical specimens from cancer patients, thus holding great potential in the clinical diagnosis of human cancer. All these features render LD-TTP an effective tool for medical diagnosis of LD polarity-related diseases.

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“…The fwhm resolution of the STED image is up to 65 ± 7 nm, which is substantially higher than the confocal image of 250 ± 6 nm (Figure c,d) and belongs to one of the leading results of LDs imaging (Table S1). − Notably, this high-resolution STED image is obtained under a relative weak STED laser intensity (16 MW cm –2 ), which is highly desired for live cell imaging with decreased photodamage. Further enhancing the fwhm resolution better than 65 nm may be realized by STED imaging with time-gated detection.…”

Section: Resultsmentioning

confidence: 99%

“…In the case of the STED nanoscopy imaging technique, it has two intrinsic requirements for fluorescent probes: (1) the probe should be able to be efficiently depleted by a STED laser; and (2) the probe should display very high photostability because of the tremendous energy of STED laser. , Since the traditional organic fluorescent probes could not satisfy these harsh demands, great efforts have been donated to develop new organic fluorescent probes capable for STED nanoscopy imaging. As a result, a few superior fluorescent probes have been reported very recently for STED nanoscopy imaging of various cellular organelles, e.g., LDs, − mitochondrial membrane, − cytoskeleton, , and so on. − Besides these organic molecular fluorescent probes, some organic/inorganic nanoparticles-based fluorescent probes have also emerged as powerful tools for STED imaging. − These works highlight the advantage of STED nanoscopy imaging over the traditional confocal/two-photon imaging, and significantly promote the relative study of cell biological on the nanoscale. However, fluorescent probes that are competent for STED nanoscopy imaging are quite limited.…”

mentioning

confidence: 99%

STED Nanoscopy Imaging of Cellular Lipid Droplets Employing a Superior Organic Fluorescent Probe

et al. 2021

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Lipid droplets (LDs) are spherical organelles that participate in numerous biological processes. In order to visualize LDs on the nanoscale, nanoscopy fluorescence imaging is considered as the most attractive technique but is substantially limited by the characteristics of fluorescent probes. Thus, the development of a superior fluorescent probe that is capable of nanoscopy fluorescence imaging has attracted enormous attention. Herein, a benzodithiophene-tetraoxide-based molecule Lipi-BDTO has been developed that can easily undergo the stimulated emission depletion (STED) process and displays high photostability. These two characteristics of fluorescent probes finely satisfy the requirements of STED nanoscopy imaging. Indeed, applying the probe for STED imaging achieves a high resolution of 65 nm, belonging to one of the leading results of LDs fluorescence imaging. Furthermore, the high photostability of this fluorescent probe enables it to monitor the dynamics of LDs by time-lapse STED imaging as well as to visualize the three-dimensional (3D) spatial distribution of LDs by 3D STED imaging. Notably, the resolution of the 3D STED image represents one of the best LDs fluorescence imaging results so far. Besides STED nanoscopy imaging, the superior utility of this fluorescent probe has been also demonstrated in two-color 3D confocal imaging and four-color confocal imaging.

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Revealing lipid droplets evolution at nanoscale under proteohormone stimulation by a BODIPY- hexylcarbazole derivative

Cited by 24 publications

References 43 publications

Structure Rigidification Promoted Ultrabright Solvatochromic Fluorescent Probes for Super-Resolution Imaging of Cytosolic and Nuclear Lipid Droplets

Structure Rigidification Promoted Ultrabright Solvatochromic Fluorescent Probes for Super-Resolution Imaging of Cytosolic and Nuclear Lipid Droplets

Lipid Droplet-Specific Fluorescent Probe for In Vivo Visualization of Polarity in Fatty Liver, Inflammation, and Cancer Models

STED Nanoscopy Imaging of Cellular Lipid Droplets Employing a Superior Organic Fluorescent Probe

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