Revealing molecular structure and orientation with Stokes vector resolved second harmonic generation microscopy

Mazumder, Nirmal; Hu, Chih Wei; Qiu, Jianjun; Foreman, Matthew R.; Romero, Carlos Macías; Török, Péter; Kao, Fu Jen

doi:10.1016/j.ymeth.2013.07.019

Cited by 30 publications

(27 citation statements)

References 48 publications

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“…However, Jones calculus is limited only for perfectly polarized light and the complete polarization state cannot be revealed due to the lack of the phase difference between the two components of the measured polarization vector. For example, use of cross-polarized two-channel detection does not allow the relative ratio of the polarized and un-polarized components of the electric field to be determined1113. For comparison, Stokes-Mueller formalism is a powerful approach that accommodates all polarization states, including incoherent, partially polarized, and unpolarized ones112021.…”

Section: Methodsmentioning

confidence: 99%

“…Stokes vector based SHG microscopy resolves the polarization states of the SH signal and allows the deduction of the molecular organization of collagen, skeletal muscle, starch etc 111213…”

mentioning

confidence: 99%

“…A Stokes vector based SHG microscopy scheme is distinct from several other previously demonstrated polarization resolved approaches in that it uses a large number of images rather than a single shot measurement11161718. The complete polarization states of the SH light of starch granules were characterized from SHG Stokes micrographs1113. The technique was implemented to characterize the polarization properties of the SH signal from starch granule during starch-water interaction.…”

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confidence: 99%

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Investigating starch gelatinization through Stokes vector resolved second harmonic generation microscopy

Mazumder

Xiang²,

Qiu

et al. 2017

Sci Rep

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The changes of the morphology during heating and the degree of crystallinity of dry and hydrated starch granules are investigated using second harmonic generation (SHG) based Stokes polarimetry. A spatial distribution of various polarization parameters, such as the degree of polarization (DOP), the degree of linear polarization (DOLP), and the degree of circular polarization (DOCP) are extracted and compared with the two dimensional second harmonic (SH) Stokes images of starch granules. The SH signal from hydrated and dry starch on heating differed significantly in DOLP and DOCP values, indicating that hydrated starch has a greater degree of ultrastructural amylopectin disorder. The detail of denaturation and the phase transition of hydrated starch demonstrate the significant influence of thermal processing.

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Section: Methodsmentioning

confidence: 99%

“…Stokes vector based SHG microscopy resolves the polarization states of the SH signal and allows the deduction of the molecular organization of collagen, skeletal muscle, starch etc 111213…”

mentioning

confidence: 99%

mentioning

confidence: 99%

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Investigating starch gelatinization through Stokes vector resolved second harmonic generation microscopy

Mazumder

Xiang²,

Qiu

et al. 2017

Sci Rep

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“…This failure can be considered an important caveat for the broad field of imaging microscopy, [30] or indeed any prospective studies aiming to extend, into the near-field regime, current far-field applications of Stokes vector analysis. [31] Finally, an alternative, rigorous measure of optical helicity based on a coupled system incorporating both a molecular emitter and detector has been evaluated within the theoretical framework of QED. The nearfield result is noted to vary with R -5 and exhibits features consistent with the familiar near-zone electric dipole-dipole coupling interaction, consistent with chiral dissymmetry in the coupled source-detector system.…”

Section: Resultsmentioning

confidence: 99%

Point source generation of chiral fields: measures of near- and far-field optical helicity

2015

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To consider the relationship between different measures of chirality in an optical field, the simplest case is considered: direct spontaneous emission of circularly polarized light by a point source. In the electromagnetic fields radiated from a suitably chiral source, such as a low-symmetry chiral molecule undergoing radiative decay, optical helicity is exhibited in the extent of a difference in left-and right-handed circular polarization components. There are several practical measures for quantifying the emergence of ensuing optical helicity, exhibiting different forms of dependence on the properties of the emitter and the positioning of a detector. By casting each measure in terms of an irreducible helicity density, connections and distinctions can be drawn between results expressible in either classical or quantum form.

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“…Later, Simpson J G developed a formalism for nonlinear optical ellipsometry using Jones matrices. 34, 35 4 Â 1 Stokes vector and 4 Â 4 Mueller matrix are used [36][37][38][39] in second harmonic generation (SHG) microscopy to understand molecular level structure of biological materials. SHG is studied recently using Double Stokes-Double Mueller Matrix formalism by di®erent groups.…”

Section: Introductionmentioning

confidence: 99%

Generalized Stokes vector for three photon process

Shaji

Ismail

Satyanarayana

et al. 2017

J. Nonlinear Optic. Phys. Mat.

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Stokes Mueller formalism is used to measure the optical properties of materials by probing the sensitive changes in the polarization state of light after scattering from the medium. The formalism can be extended to nonlinear scattering processes involving two and three photon processes also. In this paper, we derive the Stokes vector, viz. triple Stokes vector analytically from quantum theory of light for a three photon process. The state of polarization of light, with three simultaneous photons, incident on a material is described by triple Stokes vector, and has 16 components involving cubes of intensities dependence. The response of a material for the scattered light in a three photon process is described by 4 × 16 Mueller matrix. Polarization in Polarization out (PIPO) experiments can be carried out to determine the elements of Mueller matrix. We identify 16 independent points on Poincare sphere and construct triple Stokes vector for each point. Four measurements are done to determine the linear Stokes vector of scattered light, where the 16 polarization states are represented by triple Stokes vector and this determines the Mueller matrix of the sample.

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Revealing molecular structure and orientation with Stokes vector resolved second harmonic generation microscopy

Cited by 30 publications

References 48 publications

Investigating starch gelatinization through Stokes vector resolved second harmonic generation microscopy

Investigating starch gelatinization through Stokes vector resolved second harmonic generation microscopy

Point source generation of chiral fields: measures of near- and far-field optical helicity

Generalized Stokes vector for three photon process

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