Revealing the Cortical Glutamatergic Neural Activity During Burst Suppression by Simultaneous wide Field Calcium Imaging and Electroencephalography in Mice

Gui, Shen; Li, Jiayan; Li, Miaowen; Shi, Liang; Lu, Jinling; Shen, Shiqian; Li, Pengcheng; Mei, Wei

doi:10.1016/j.neuroscience.2021.06.036

Cited by 8 publications

(4 citation statements)

References 56 publications

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“…Animals then underwent sham or FLIT surgery, followed by intravital two-photon imaging, with both lower magnification (×4, wide-field) and higher magnification (×20). Mice were head-fixed without the use of anesthesia, as anesthetics are known to suppress cortical neural activity ( 25 ). More important, the animals were free of experimentally imposed stimuli to minimize evoked pain.…”

Section: Resultsmentioning

confidence: 99%

Highly synchronized cortical circuit dynamics mediate spontaneous pain in mice

Ding¹,

Fischer²,

Chen³

et al. 2023

Journal of Clinical Investigation

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Cortical neural dynamics mediate information processing for the cerebral cortex, implicated in fundamental biological processes, such as vision and olfaction, in addition to neurological and psychiatric diseases. Spontaneous pain is a key feature of human neuropathic pain. Whether spontaneous pain pushes cortical network into an aberrant state, and if so, whether it can be brought back to a 'normal' operating range to ameliorate pain are unknown. Using a clinically relevant mouse model of neuropathic pain with spontaneous pain-like behavior, we report that orofacial spontaneous pain activated a specific area within the primary somatosensory cortex (S1), displaying synchronized neural dynamics revealed by intravital two-photon calcium imaging.This synchronization was underpinned by local GABAergic interneuron hypoactivity. Paininduced cortical synchronization could be attenuated by manipulating local S1 networks or clinically effective pain therapies. Specifically, both chemogenetic inhibition of pain-related c-Fosexpressing neurons, and selective activation of GABAergic interneurons, significantly attenuated S1 synchronization. Clinically effective pain therapies including carbamazepine and nerve root decompression could also dampen S1 synchronization. More importantly, restoring a 'normal' range of neural dynamics, through attenuating pain-induced S1 synchronization, alleviated painlike behavior. These results suggest spontaneous pain pushes S1 regional network into a synchronized state, whereas reversal of this synchronization alleviates pain.

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Section: Resultsmentioning

confidence: 99%

Highly synchronized cortical circuit dynamics mediate spontaneous pain in mice

Ding¹,

Fischer²,

Chen³

et al. 2023

Journal of Clinical Investigation

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“…Finally, a perspective would be to follow a similar approach at a larger scale, by relating signals such as the wide-field calcium signal, with largescale electric signals such as the EcoG or the electro-encephalogram (EEG) (e.g. as in 16,38 ). The present observation that the field-of-view signal and neuropil signal largely overlap, shows that the contribution of somata www.nature.com/scientificreports/ is comparatively small.…”

Section: Discussionmentioning

confidence: 99%

Assessing brain state and anesthesia level with two-photon calcium signals

Tort-Colet

Resta²,

Montagni³

et al. 2023

Sci Rep

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Brain states, such as wake, sleep, or different depths of anesthesia are usually assessed using electrophysiological techniques, such as the local field potential (LFP) or the electroencephalogram (EEG), which are ideal signals for detecting activity patterns such as asynchronous or oscillatory activities. However, it is technically challenging to have these types of measures during calcium imaging recordings such as two-photon or wide-field techniques. Here, using simultaneous two-photon and LFP measurements, we demonstrate that despite the slower dynamics of the calcium signal, there is a high correlation between the LFP and two-photon signals taken from the neuropil outside neuronal somata. Moreover, we find the calcium signal to be systematically delayed from the LFP signal, and we use a model to show that the delay between the two signals is due to the physical distance between the recording sites. These results suggest that calcium signals alone can be used to detect activity patterns such as slow oscillations and ultimately assess the brain state and level of anesthesia.

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“…Burst suppression is a common EEG pattern during deep general anesthesia 28 and deep coma state after brain trauma or cardiac arrest 44 . The burst suppression EEG hallmark was considered due to global anatomical or functional network connectivity disruptions 45 . We, therefore, examined the neurotransmitter release and Ca 2+ activity during unconsciousness with burst suppression in the cerebello-M1 loop.…”

Section: Resultsmentioning

confidence: 99%

Cerebellar Purkinje cell firing promotes conscious recovery from anesthesia state through coordinating neuronal communications with motor cortex

Zhu,

Chen,

Liu

et al. 2024

Theranostics

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Background: The neurobiological basis of gaining consciousness from unconscious state induced by anesthetics remains unknown. This study was designed to investigate the involvement of the cerebello-thalamus-motor cortical loop mediating consciousness transitions from the loss of consciousness (LOC) induced by an inhalational anesthetic sevoflurane in mice. Methods: The neural tracing and fMRI together with opto - chemogenetic manipulation were used to investigate the potential link among cerebello-thalamus-motor cortical brain regions. The fiber photometry of calcium and neurotransmitters, including glutamate (Glu), γ-aminobutyric acid (GABA) and norepinephrine (NE), were monitored from the motor cortex (M1) and the 5 th lobule of the cerebellar vermis (5Cb) during unconsciousness induced by sevoflurane and gaining consciousness after sevoflurane exposure. Cerebellar Purkinje cells were optogenetically manipulated to investigate their influence on consciousness transitions during and after sevoflurane exposure. Results: Activation of 5Cb Purkinje cells increased the Ca 2+ flux in the M1 CaMKIIα + neurons, but this increment was significantly reduced by inactivation of posterior and parafascicular thalamic nucleus . The 5Cb and M1 exhibited concerted calcium flux, and glutamate and GABA release during transitions from wakefulness, loss of consciousness, burst suppression to conscious recovery. Ca 2+ flux and Glu release in the M1, but not in the 5Cb, showed a strong synchronization with the EEG burst suppression, particularly, in the gamma-band range. In contrast, the Glu, GABA and NE release and Ca 2+ oscillations were coherent with the EEG gamma band activity only in the 5Cb during the pre-recovery of consciousness period. The optogenetic activation of Purkinje cells during burst suppression significantly facilitated emergence from anesthesia while the optogenetic inhibition prolonged the time to gaining consciousness. Conclusions: Our data indicate that cerebellar neuronal communication integrated with motor cortex through thalamus promotes consciousness recovery from anesthesia which may likely serve as arousal regulation.

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Revealing the Cortical Glutamatergic Neural Activity During Burst Suppression by Simultaneous wide Field Calcium Imaging and Electroencephalography in Mice

Cited by 8 publications

References 56 publications

Highly synchronized cortical circuit dynamics mediate spontaneous pain in mice

Highly synchronized cortical circuit dynamics mediate spontaneous pain in mice

Assessing brain state and anesthesia level with two-photon calcium signals

Cerebellar Purkinje cell firing promotes conscious recovery from anesthesia state through coordinating neuronal communications with motor cortex

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