Revealing the dynamics of a mitochondrial microenvironment during apoptosis under two-photon fluorescence lifetime microscopy using a cyclic iridium(<scp>iii</scp>) complex

Lin, Ying; Zhu, Tong; Wang, Shu Jing; Feng, Zhihui; Cao, Hongzhi; Tian, Yupeng; Tian, Xiaohe

doi:10.1039/d2qi01109c

Cited by 4 publications

(3 citation statements)

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“…Tian and co-workers have developed an iridium(III) complex bearing a long and flexible alkyl chain (667) that can monitor apoptosis by tracking mitochondrial viscosity. 1251 Additionally, the complex has been used to examine the differences in the microenvironment of tumor and normal tissues in a mouse model, revealing a higher viscosity level in the tumor microenvironment. Zhang, Zhang, and co-workers have developed two mitochondria-targeting viscosity-responsive iridium(III) complexes ( 668) for PDT applications.…”

Section: Viscositymentioning

confidence: 99%

“…On the basis of its viscosity-sensitive emission lifetime and large TPA cross-section ( δ 750nm = 444 GM), complex 666f has been utilized for real-time monitoring and quantitative measurements of changes in mitochondrial viscosity using TP-PLIM, which shows that dysfunctional mitochondria experience a significant but heterogeneous increase in viscosity (Figure ). Tian and co-workers have developed an iridium(III) complex bearing a long and flexible alkyl chain ( 667 ) that can monitor apoptosis by tracking mitochondrial viscosity . Additionally, the complex has been used to examine the differences in the microenvironment of tumor and normal tissues in a mouse model, revealing a higher viscosity level in the tumor microenvironment.…”

Section: Luminescent Transition Metal Complexes As Probes For Microen...mentioning

confidence: 99%

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Shining New Light on Biological Systems: Luminescent Transition Metal Complexes for Bioimaging and Biosensing Applications

Lee,

2024

Chem. Rev.

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Luminescence imaging is a powerful and versatile technique for investigating cell physiology and pathology in living systems, making significant contributions to life science research and clinical diagnosis. In recent years, luminescent transition metal complexes have gained significant attention for diagnostic and therapeutic applications due to their unique photophysical and photochemical properties. In this Review, we provide a comprehensive overview of the recent development of luminescent transition metal complexes for bioimaging and biosensing applications, with a focus on transition metal centers with a d 6 , d 8 , and d 10 electronic configuration. We elucidate the structure−property relationships of luminescent transition metal complexes, exploring how their structural characteristics can be manipulated to control their biological behavior such as cellular uptake, localization, biocompatibility, pharmacokinetics, and biodistribution. Furthermore, we introduce the various design strategies that leverage the interesting photophysical properties of luminescent transition metal complexes for a wide variety of biological applications, including autofluorescence-free imaging, multimodal imaging, organelle imaging, biological sensing, microenvironment monitoring, bioorthogonal labeling, bacterial imaging, and cell viability assessment. Finally, we provide insights into the challenges and perspectives of luminescent transition metal complexes for bioimaging and biosensing applications, as well as their use in disease diagnosis and treatment evaluation.

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Section: Viscositymentioning

confidence: 99%

Section: Luminescent Transition Metal Complexes As Probes For Microen...mentioning

confidence: 99%

Shining New Light on Biological Systems: Luminescent Transition Metal Complexes for Bioimaging and Biosensing Applications

Lee,

2024

Chem. Rev.

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“…As fluorescence lifetime is an intrinsic property of fluorescence probes, FLIM is not influenced by concentration variations, which can be affected by cell volume changes or photobleaching . Additionally, the sensitivity of fluorescence lifetime to microenvironments enables the visualization and quantification of biophysical parameters such as polarity and viscosity within cells. − Thus, two-photon FLIM (TP-FLIM) is a valuable tool for measuring changes or differences of microenvironments in subcellular regions or in vivo . − To our knowledge, although a few studies have reported the correlation between the polarity change of LDs and the fluorescence lifetime by using one-photon FLIM, , no research has yet reported the polarity differences of LDs in vitro and in vivo by using TP-FLIM. In this study, TBPCPP, a polarity-sensitive two-photon fluorescence lifetime probe for LDs with a donor (D)-acceptor (A)-π-acceptor (A) structure was designed and synthesized by using a facile method (Scheme ).…”

Section: Introductionmentioning

confidence: 99%

Polarity-Sensitive Probe for Two-Photon Fluorescence Lifetime Imaging of Lipid Droplets In Vitro and In Vivo

Sha,

Liu,

Zheng

et al. 2023

Anal. Chem.

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Lipid droplets (LDs) are crucial organelles used to store lipids and participate in lipid metabolism in cells. The abnormal aggregation and polarity change of LDs are associated with the occurrence of diseases, such as steatosis. Herein, the polarity-sensitive probe TBPCPP with a donor–acceptor-π-acceptor (D-A-π-A) structure was designed and synthesized. The TBPCPP has a large Stokes shift (∼220 nm), excellent photostability, high LD targeting, and considerable two-photon absorption (TPA) cross-section (∼226 GM), enabling deep two-photon imaging (∼360 μm). In addition, the fluorescence lifetime of TBPCPP decreases linearly with increasing solvent polarity. Therefore, with the assistance of two-photon fluorescence lifetime imaging microscopy (TP-FLIM), TBPCPP has successfully achieved not only the visualization of polarity changes caused by LD accumulation in HepG-2 cells but also lipid-specific imaging and visualization of different polarities in lipid-rich regions in zebrafish for the first time. Furthermore, TP-FLIM revealed that the polarity gradually decreases during steatosis in HepG-2 cells, which provided new insights into the diagnosis of steatosis.

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Fluorescence microscopic platforms imaging mitochondrial abnormalities in neurodegenerative diseases

Wang

Liu

2023

Advanced Drug Delivery Reviews

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Revealing the dynamics of a mitochondrial microenvironment during apoptosis under two-photon fluorescence lifetime microscopy using a cyclic iridium(iii) complex

Abstract: Mitochondria-mediated apoptosis is a major mode of cell death and is inextricably linked to various pathological processes such as tumorigenesis. However, there is still a paucity of non-toxic tools that...

Cited by 4 publications

References 38 publications

Shining New Light on Biological Systems: Luminescent Transition Metal Complexes for Bioimaging and Biosensing Applications

Shining New Light on Biological Systems: Luminescent Transition Metal Complexes for Bioimaging and Biosensing Applications

Polarity-Sensitive Probe for Two-Photon Fluorescence Lifetime Imaging of Lipid Droplets In Vitro and In Vivo

Fluorescence microscopic platforms imaging mitochondrial abnormalities in neurodegenerative diseases

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