Reveals of New Candidate Active Components in Hemerocallis Radix and Its Anti-Depression Action of Mechanism Based on Network Pharmacology Approach

Abstract

The global depression population is showing a significant increase. Hemerocallis fulva L. is a common Traditional Chinese Medicine (TCM). Its flower buds are known to have ability to clear away heat and dampness, detoxify, and relieve depression. Ancient TCM literature shows that its roots have a beneficial effect in calming the spirit and even the temper in order to reduce the feeling of melancholy. Therefore, it is inferred that the root of Hemerocallis fulva L. can be used as a therapeutic medicine for depression. This study aims to uncover the pharmacological mechanism of the antidepressant effect of Hemerocallis Radix (HR) through network pharmacology method. During the analysis, 11 active components were obtained and screened using ADME—absorption, distribution, metabolism, and excretion— method. Furthermore, 267 HR targets and 740 depressive disorder (DD) targets were gathered from various databases. Then protein–protein interaction (PPI) network of HR and DD targets were constructed and cluster analysis was applied to further explore the connection between the targets. In addition, gene ontology (GO) enrichment and pathway analysis was applied to further verify that the biological process related to the target protein is associated with the occurrence of depression disorder. In conclusion, the most important bioactive components—anthraquinone, kaempferol, and vanillic acid—can alleviate depression symptoms by regulating MAOA, MAOB, and ESR1. The proposed network pharmacology strategy provides an integrating method to explore the therapeutic mechanism of multi-component drugs on a systematic level.

A Combined Network Pharmacology and Molecular Docking Approach to Investigate Candidate Active Components and Multitarget Mechanisms of Hemerocallis Flowers on Antidepressant Effect

“…These five screening targets have been shown to be highly correlated with depression. Monoamine oxidases (MAOs) play a crucial role during the development of various mental diseases [ 8 ]. There are two MAO isozymes, MAOA and MAOB.…”

“…It was first recorded in the publication Supplement to Compendium of Materia Medica (Bencao Gangmu Shiyi). The root, seeding, and flower of Hemerocallis fulva L. are considered to have sweet, cool, and nontoxic properties and to be associated with the spleen, liver, and bladder meridians [ 8 ]. Previous investigations revealed its predominant chemical constituents including alkaloids [ 9 ], flavones [ 10 ], terpenes [ 11 ], steroidal saponins [ 12 ], and phenolic glycosides [ 13 ].…”

A network pharmacology based approach for predicting active ingredients and potential mechanism of Lianhuaqingwen capsule in treating COVID-19

“…Components with OB ≥30% and DL ≥0.18 are more likely to be drugs. Hence these two parameters were set as criteria to select candidate ingredients in TCMSP 18 , 19 . However, some herbs can't be found in the database, such as Rhodiola rosea, Mentha and Gypsum, the three herbs were retrieved using BATMAN-TCM and TCMID databases 20 , 21 .…”

Systemic elucidation on the potential bioactive compounds and hypoglycemic mechanism of Polygonum multiflorum based on network pharmacology

“…The intersection targets were input into the String database to obtain the interaction results among the targets [28], and the visual analysis results were obtained by Cytoscape 3.2.1. Cytoscape-hubba was used to screen hub targets and Cytoscape-clusterONE was used for cluster analysis.…”