Reversal effect of Dioscin on multidrug resistance in human hepatoma HepG2/adriamycin cells

Sun, Bu Tong; Li, Zheng; Bao, Yong; Yu, Chun; Wu, Yin; Meng, Xiang; Li, Yu Xin

doi:10.1016/j.ejphar.2010.12.018

Cited by 44 publications

(25 citation statements)

References 22 publications

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“…Chemoresistance has significantly limited treatment efficacy for many cancers 34–36. Recently, miRNAs were discovered to play a role in cisplatin resistance in various cancers, including ATC 37.…”

Section: Discussionmentioning

confidence: 99%

miR-182 promotes tumor growth and increases chemoresistance of human anaplastic thyroid cancer by targeting tripartite motif 8

Liu

Zhang

Shi

et al. 2017

OTT

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Chemotherapy is one of the most effective forms of cancer treatment and has been used in the treatment of various malignant tumors. We have gained significant insight into the mechanisms of chemoresistance but the details of the molecular mechanisms remain unclear. In the present study, we found that tripartite motif 8 (TRIM8) expression was downregulated in anaplastic thyroid cancer (ATC) tissues and cell lines. This downregulation of TRIM8 was significantly correlated with the upregulation of miR-182 in human ATC tissues. Bioinformatic analysis and luciferase reporter assays identified TRIM8 as a direct target of miR-182 in ATC. A functional assay using an MTT assay and colony formation showed that miR-182 induced cellular growth by repressing TRIM8 expression. Additionally, overexpressed miR-182 contributed to the chemoresistance of ATC cells by the repression of TRIM8 expression. In conclusion, these results demonstrate that miR-182/TRIM8 may be a therapeutic target for the treatment of chemoresistant human thyroid papillary cancer.

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Section: Discussionmentioning

confidence: 99%

miR-182 promotes tumor growth and increases chemoresistance of human anaplastic thyroid cancer by targeting tripartite motif 8

Liu

Zhang

Shi

et al. 2017

OTT

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“…Previous studies concerning the drug resistance of HCC cells have used doxorubicin-resistant cell lines that were generated through constant exposure to rising levels of Hepatocellular carcinoma cells surviving doxorubicin treatment exhibit increased migratory potential and resistance to doxorubicin re-treatment in vitro doxorubicin (13,(20)(21)(22). By contrast, the aim of the present study was to analyze the effects of single-step doxorubicin treatment on surviving HCC cells in vitro, mimicking the situation of HCC cells surviving TACE treatment.…”

Section: Introductionmentioning

confidence: 90%

Hepatocellular carcinoma cells surviving doxorubicin treatment exhibit increased migratory potential and resistance to doxorubicin re-treatment inï¿½vitro

et al. 2018

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Abstract. Transarterial chemoembolization (TACE) is an established therapeutic approach for the treatment of hepatocellular carcinoma (HCC). Although patients who undergo TACE may have prolonged survival, there are indications that the malignancy of residual HCC tissue can increase subsequent to the procedure. Although hypoxia, which occurs during TACE due to ischemia, is known to contribute to angiogenesis, little is known with regard to the undesirable effects of chemotherapeutic agents on residual HCC cells. Doxorubicin is one of the most commonly used drugs in TACE. The aim of the present study was to analyze alterations in Hep3B and HepG2 human HCC cell lines surviving doxorubicin treatment in vitro. Initially, the toxic concentration range was determined, and doxorubicin was subsequently applied in concentrations that killed >80% of the HCC cells. During the first days subsequent to treatment, surviving cells had higher expression levels of the epithelial-mesenchymal transition marker SNAIL, and exhibited increased migratory activity compared with control cells. At 3 weeks after the first doxorubicin treatment, surviving HCC cells tolerated significantly higher doxorubicin concentrations compared with control cells. As a potential explanation for this doxorubicin resistance, significantly increased mRNA expression levels of ATP-binding cassette ABCB1 (multidrug resistance protein 1) and ABCC1 (multidrug resistance-associated protein 1) were observed by reverse transcription-quantitative polymerase chain reaction. In summary, these findings indicate that, following TACE treatment, hypoxia as well as doxorubicin may induce a more malignant phenotype in surviving HCC cells and decrease susceptibility to further chemotherapeutic treatment.

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“…The ABCB1 promoter reporter plasmid was constructed, as previously described (Sun et al, 2011). Human genomic DNA was prepared from human whole blood using the phenol/chloroform extraction protocol.…”

Section: Construction Of Abcb1 Promoter Reporter Plasmid and Cell Tramentioning

confidence: 99%

Psoralen reverses docetaxel-induced multidrug resistance in A549/D16 human lung cancer cells lines

Hsieh

Chen

et al. 2014

Phytomedicine

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Reversal effect of Dioscin on multidrug resistance in human hepatoma HepG2/adriamycin cells

Cited by 44 publications

References 22 publications

miR-182 promotes tumor growth and increases chemoresistance of human anaplastic thyroid cancer by targeting tripartite motif 8

miR-182 promotes tumor growth and increases chemoresistance of human anaplastic thyroid cancer by targeting tripartite motif 8

Hepatocellular carcinoma cells surviving doxorubicin treatment exhibit increased migratory potential and resistance to doxorubicin re-treatment inï¿½vitro

Psoralen reverses docetaxel-induced multidrug resistance in A549/D16 human lung cancer cells lines

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Reversal effect of Dioscin on multidrug resistance in human hepatoma HepG2/adriamycin cells

Cited by 44 publications

References 22 publications

miR-182 promotes tumor growth and increases chemoresistance&nbsp;of human anaplastic thyroid cancer by targeting tripartite motif 8

miR-182 promotes tumor growth and increases chemoresistance&nbsp;of human anaplastic thyroid cancer by targeting tripartite motif 8

Hepatocellular carcinoma cells surviving doxorubicin treatment exhibit increased migratory potential and resistance to doxorubicin re-treatment inï¿½vitro

Psoralen reverses docetaxel-induced multidrug resistance in A549/D16 human lung cancer cells lines

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miR-182 promotes tumor growth and increases chemoresistance of human anaplastic thyroid cancer by targeting tripartite motif 8

miR-182 promotes tumor growth and increases chemoresistance of human anaplastic thyroid cancer by targeting tripartite motif 8