Reversal effects of some safe dietary supplements on lead contaminated diet induced impaired growth and associated parameters in Nile tilapia

Abdel-Rahman

et al. 2021

Aquaculture Research

Self Cite

A 12‐week feeding experiment was performed to examine the impacts of partial or complete substitution of soybean meal (SM) with leaf protein concentrate from carrot and sugar beet (CLPC and SLPC) on growth, blood constituents and body composition in Oreochromis niloticus. Five isocaloric (gross energy, 4300 kcal/kg) and isonitrogenous (crude protein, 320 g/kg) diets were formulated. The basal diet was formulated to contain SM at 350 g/kg diet. SM was replaced by 50% and 100% of SLPC and CLPC (SLPC‐50, CLPC‐50, SLPC‐100 and CLPC‐100 respectively). The results revealed that there were no significant changes in the growth indices between fish fed on basal diet and SLPC‐50 and CLPC‐100 diets. The condition factor was significantly increased in all dietary SLPC and CLPC substitution levels. Feed intake, feed conversion ratio and survival rate were not altered by various dietary SLPC and CLPC levels. The inclusion levels of SLPC and CLPC in fish feed did not significantly affect most blood biochemical and haematological parameters. Except for the SLPC‐50 group, all substituted groups displayed a significant depletion in the crude lipid content compared with the non‐substituted group. Fish fed on CLPC‐100 diet recorded the lowest feeding cost and best profit margin compared with other experimental groups. In summary, the findings demonstrated that SLPC and CLPC could replace 50% and 100% of SM, respectively in O. niloticus feeds without any negative effects on growth and blood constituents. Besides, the total replacement of SM with CLPC was more efficient in producing low cost and healthy aquafeed.

Section: Introductionmentioning

confidence: 99%

Evaluation of leaf protein concentrate from Beta vulgaris and Daucus carota as a substitute for soybean meal in Oreochromis niloticus fingerlings diets

Abdel-Rahman

et al. 2021

Aquaculture Research

Self Cite

“…Diet formulation was adjusted by manipulating the bentonite content 111 – 113 . However, some studies showed that the bentonite in feed can alleviate toxicity (e.g., induced by dietary aflatoxin B1, plumbum and cadmium) by decreasing toxic substances residues in fish bodies, rehabilitating the enzyme activity and modifying the function of kidney and liver in fish 114 – 117 . These results might explain the reported beneficial effects of feeding bentonite to fish in some cases 118 , 119 .…”

Section: Discussionmentioning

confidence: 99%

Dietary carbohydrates influence muscle texture of olive flounder Paralichthys olivaceus through impacting mitochondria function and metabolism of glycogen and protein

Liu

Deng

Pan

et al. 2020

Sci Rep

The present study was conducted to estimate the effects of dietary carbohydrates on muscle quality and the underlying mechanisms. Six isonitrogenous and isolipidic diets were formulated to contain graded levels of carbohydrates (0%, 8%, 12%, 16%, 20% and 24%, respectively). These diets were named as C0, C8, C12, C16, C20 and C24, respectively. After a 10-week feeding trial, results showed that the muscle pH, liquid holding capacity (LHC) and hardness were significantly decreased by the increasing dietary carbohydrate levels. Dietary carbohydrates significantly decreased the muscle fibre diameter, and the highest value was found in the C0 group. Accumulated glycogen and degenerated mitochondrial cristae were observed in the C24 group. Significantly higher contents of protein carbonyls were observed in the C20 group and C24 group (P < 0.05). There was a significant decrease of mtDNA copy number in the C24 group compared with that in the C0 and C8 groups. The AMP/ATP ratio in muscle decreased first and then increased with the increasing dietary carbohydrate levels. The dietary incorporation of carbohydrate significantly reduced the expression of opa1, pygm and genes involved in myogenesis (myf5 and myog). Meanwhile, proteolysis-related genes (murf-1, mafbx, capn2 and ctsl), pro-inflammatory cytokines (il-6 and tnf-α) and mstn were significantly up-regulated. In the C24 group, significant increase of phosphorylation of AMPK (Thr172), up-regulation of PGC-1α and GLUT4 were observed, while the phosphorylation level of S6 (Ser235/236) was significantly decreased. It was concluded that excessive dietary carbohydrate level (24%) had negative impacts on mitochondria function and promoted glycogen accumulation, and thereafter influenced the muscle quality of olive flounder. The activation of AMPK as well as the upregulation of PGC-1α and GLUT4 was the key mechanism.

“…Aquaculture is a globally essential industry that provides food to the world's rapidly growing population, in addition to being a good source of low-cost animal protein [1]. Nile tilapia (Oreochromis niloticus) is the second-largest aquatic species cultivated worldwide [2,3]. The overall production of farmed Nile tilapia in Egypt accounts for 71.2 percent of all farmed Nile tilapia worldwide [4] and is the source of the most common strains and species used in commercial aquaculture.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Antibiogram of Vibrio parahaemolyticus and Aeromonas hydrophila in the Flesh of Nile Tilapia, with Special Reference to Their Virulence Genes Detected Using Multiplex PCR Technique

Zaher

Nofal²,

Hendam

et al. 2021

Antibiotics

Vibrio parahaemolyticus and Aeromonas hydrophila are major public health problems and the main cause of bacterial disease in Nile tilapia (Oreochromis niloticus). This study was conducted to determine the prevalence, antibiotic resistance and some virulence genes of both V. parahaemolyticus and A. hydrophila isolates from Nile tilapia. From Manzala Farm at Dakahlia governorate, 250 freshwater fish samples were collected. The confirmed bacterial isolates from the examined Nile tilapia samples in the study were 24.8% (62/250) for V. parahaemolyticus and 19.2% (48/250) for A. hydrophila. multiplex PCR, revealing that the tlh gene was found in 46.7% (29/62) of V. parahaemolyticus isolates, while the tdh and trh virulence genes were found in 17.2% (5/29). Meanwhile, 39.5% (19/48) of A. hydrophila isolates had the 16s rRNA gene and 10.5% (2/19) had the aerA and ahh1 virulence genes. The Multiple Antibiotic Resistance indices of V. parahaemolyticus and A. hydrophila were 0.587 and 0.586, respectively. In conclusion, alternative non-antibiotic control strategies for bacterial infections in farmed fish should be promoted to avoid multidrug-resistant bacteria. Therefore, it is suggested that farmers should be skilled in basic fish health control and that molecular detection methods are more rapid and cost-effective than bacteriological methods.