Reversal of 5-flouroucial resistance by adenovirus-mediated transfer of wild-typep53 gene in multidrug-resiatant human colon carcinoma LoVo/5-FU cells

Abstract

Wide-type p53 gene has a remarkable reversal activity for the high expression of MDR1 gene in colorectal cancers. The reversal effects seem to be in a time dependent manner. It might have good prospects in clinical application.

Inhibition of Transient Receptor Potential Channel 5 Reverses 5-Fluorouracil Resistance in Human Colorectal Cancer Cells

“…In this study, ABCB1 expression was higher in HCT-8/5-Fu and LoVo/5-Fu cells than in the parental HCT-8 and LoVo cells, consistent with previous studies (34,35). Also, TrpC5 was entry through TrpC5.…”

Mir-148a Improves Response to Chemotherapy in Sensitive and Resistant Oesophageal Adenocarcinoma and Squamous Cell Carcinoma Cells

“…One known substrate of CYP3A4 is cisplatin, 41 and overexpression of MDR1 with consequent elevated expression of its product P-gp has been shown to result in an increased efflux of, for example, 5-FU in malignant cells. 46 There are limitations which should be considered when interpreting the results of our study. The most important limitation is the restriction to only two oesophageal cancer cell lines (OE19 and KYSE410, respectively).…”

Synergistic anticancer effect of rAd/P53 combined with 5-fluorouracil or iodized oil in the early therapeutic response of human colon cancer in vivo

“…The adenovirus-mediated transfer of the wildtype p53 gene into human colon carcinoma LoVo/5-FU cells and human hepatocellular carcinoma Bel7402/5-FU cells can reverse multidrug-resistance (Li et al, 2004;Yu et al, 2004). The 5637 bladder cancer cell line exhibited a synergistic killing effect when the rAAV-wt- Note: n = number of tumors.…”

“…One of the mechanisms of p53 therapy for tumors is reversing the resistance of cancer cells to chemotherapeutic agents and radiation therapy, or increasing the sensitivity of cancer cells to chemotherapeutic agents and radiation therapy (Bookstein et al, 1996;Levine et al, 1991;Yu et al, 2004). The adenovirus-mediated transfer of the wildtype p53 gene into human colon carcinoma LoVo/5-FU cells and human hepatocellular carcinoma Bel7402/5-FU cells can reverse multidrug-resistance (Li et al, 2004;Yu et al, 2004).…”