Reverse micellar extraction of bromelain from <i>Ananas comosus</i> L. Merryl

Hemavathi, A. B.; Hebbar, H. Umesh; Raghavarao, K.S.M.S.

doi:10.1002/jctb.1769

Cited by 30 publications

(12 citation statements)

References 25 publications

(25 reference statements)

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“…Several reports on the application of RME for separation and purification of biomolecules are available for model systems (4)(5)(6)(7)(8). In recent years, a few studies on RME of biomolecules from natural=real systems have been reported (9)(10)(11)(12)(13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

Reverse Micellar Extraction of Bromelain from Pineapple (Ananas comosusL. Merryl) Waste: Scale-up, Reverse Micelles Characterization and Mass Transfer Studies

Hebbar

Hemavathi

Sumana

et al. 2011

Separation Science and Technology

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Scale-up studies for phase transfer mode of reverse micellar extraction are attempted for the separation and primary purification of bromelain (EC 3.4.22.33) from pineapple (Ananas comosus L. Merryl) waste. Characterization of reverse micelles and mass transfer studies for the real system has been attempted for the first time. Scale-up of the extraction process employing commercial grade surfactant cetyltrimethylammonium bromide (CTAB) and solvent isooctane resulted in purification of 2.43 fold with an activity recovery 81.3%. The reverse micellar size estimated using empirical and geometrical models indicated that the reverse micelles are large enough (R m ¼ 7.2-9.6 nm) to host bromelain molecules that are relatively smaller in size ($1.67 nm). The studies on the kinetics of mass transfer indicated a relatively slower rate (by $34%) of mass transfer in case of back extraction compared to forward extraction. Process scale-up did not significantly affect the extraction efficiency whereas purity of phase components played a major role. The mass transfer across the phases was high in the initial period of mixing for both forward and back extractions.

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Section: Introductionmentioning

confidence: 99%

Reverse Micellar Extraction of Bromelain from Pineapple (Ananas comosusL. Merryl) Waste: Scale-up, Reverse Micelles Characterization and Mass Transfer Studies

Hebbar

Hemavathi

Sumana

et al. 2011

Separation Science and Technology

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“…Although the exact mechanism of the cosolvent is still not quite clear, there are reports which indicate that co-solvent molecules might have inserted between the molecules of the surfactant, thereby decreasing the interaction between surfactant head groups (Hemavathi et al, 2007). Hemavathi, Hebbar, and Raghavarao (2007) studied the effect of butanol concentration on forward extraction of bromelain of pineapple using CTAB or AOT and butanol systems and reported that a decrease in extraction efficiency occurred above 15% (v v -1 ) of butanol concentration. As H 2 O SDS -1 ratio increased, the extraction efficiency also increased.…”

Section: Model Adjustment (Ccrd Results)mentioning

confidence: 99%

“…SDS is an anionic surfactant and an FDAapproved food additive (FDA 21 CFR 172.822) (Predmore & Li, 2011). With the addition of the short chain alcohol butanol, a stable micellar system may be established at room temperature and the alcohol is used in protein recovery (Krei & Hustedt, 1992;Hemavathi, Hebbar, & Raghavarao, 2007 …”

Section: Introductionmentioning

confidence: 99%

<b>Optimization of protein extraction process from jackfruit seed flour by reverse micelle system

et al. 2016

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ABSTRACT. The extraction of protein from flour of jackfruit seeds by reverse micelles was evaluated. Reverse micelle system was composed of sodium dodecyl sulfate (SDS) as surfactant, butanol as solvent, and water. The effects of stirring time, temperature, molar ratio H 2 O SDS -1 , concentration of butanol (mass percentage) and flour mass were tested in batch systems. Based on the adjusted linear regression model, only butanol concentration provided optimum extraction conditions (41.16%). Based on the analysis of surface response, the best extraction yield could be obtained at 25°C, stirring time of 120 min, mass of flour of 100 mg, and a ratio H 2 O SDS -1 of 50. Experimental results showed that a 79.00% extraction yield could be obtained.

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“…RME is a two‐step purification technique that involves solubilization of biomolecules into reverse micelles (forward extraction) and recovery of these biomolecules from reverse micelles to a fresh stripping phase (back extraction). Studies on RME of biomolecules from ‘model' as well as ‘real' systems have been reported by various research groups …”

Section: Introductionmentioning

confidence: 99%

Process optimization for reverse micellar extraction of stem bromelain with a focus on back extraction

Dhaneshwar

Chaurasiya

Hebbar

2014

Biotechnology Progress

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In the current study, reverse micellar extraction (RME) for the purification of stem bromelain was successfully achieved using the sodium bis(2-ethylhexyl) sulfosuccinate (AOT)/isooctane system. A maximum forward extraction efficiency of 58.0% was obtained at 100 mM AOT concentration, aqueous phase pH of 8.0 and 0.2 M NaCl. Back extraction studies on altering stripping phase pH and KCl concentration, addition of counter-ion and iso-propyl alcohol (IPA) and mechanical agitation with glass beads indicated that IPA addition and agitation with glass beads have significant effects on extraction efficiency. The protein extraction was higher (51.9%) in case of the IPA (10% v/v) added system during back extraction as compared to a cetyltrimethylammonium bromide (100 mM) added system (9.42%). The central composite design technique was used to optimize the back extraction conditions further. Concentration of IPA, amount of glass beads, mixing time, and agitation speed (in rpm) were the variables selected. IPA concentration of 8.5% (v/v), glass bead concentration of 0.6 (w/v), and mixing time of 45 min at 400 rpm resulted in higher back extraction efficiency of 45.6% and activity recovery of 88.8% with purification of 3.04-fold. The study indicated that mechanical agitation using glass beads could be used for destabilizing the reverse micelles and release of bromelain back into the fresh aqueous phase.

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Reverse micellar extraction of bromelain from Ananas comosus L. Merryl

Cited by 30 publications

References 25 publications

Reverse Micellar Extraction of Bromelain from Pineapple (Ananas comosusL. Merryl) Waste: Scale-up, Reverse Micelles Characterization and Mass Transfer Studies

Reverse Micellar Extraction of Bromelain from Pineapple (Ananas comosusL. Merryl) Waste: Scale-up, Reverse Micelles Characterization and Mass Transfer Studies

<b>Optimization of protein extraction process from jackfruit seed flour by reverse micelle system

Process optimization for reverse micellar extraction of stem bromelain with a focus on back extraction

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