Reverse‐phase HPLC method for the quantification of two antihyperglycemic glycolipids in Oplismenus burmannii

Abstract

Glycolipids and sphingolipids are well known for their diverse biological activities like anticancer, anti-inflammatory, antistress, anti-HIV, hepatoprotective and antimicrobial. The present study deals with the activity-guided isolation and characterization of two antihyperglycemic glycolipids, (2S)-1,2-di-O-octadecanoyl-3-O-[α-d-galctopyranosyl-(1'' → 6')-O-β-d-galactopyranosyl] glycerol (1) and 1-O-β-d-glucopyranosyl-(2S,3S,4R,8E)-2-[(2R)-2-hydroxy-tetracosanoylamino]-2,3,4-octadecanetriol-8-ene (2) from Oplismenus burmannii and the development of a simple and validated reverse-phase HPLC analytical method for their quantification in the methanolic extracts of O. burmannii. The marker compounds 1 and 2 were isolated from the methanolic extract of O. burmannii and characterized on the basis of their spectroscopic data. Their antihyperglycemic potential was evaluated by determining their glucose uptake-stimulating potential in L6-GLUT4myc myotube cells. Finally, these analytes were separated on a Waters Spherisorb ODS 2 column with a binary gradient of methanol and water at a constant flow rate of 0.8 mL/min and detected using a photodiode array detector at 230 nm. The calibration curve was linear (r(2) > 0.999) over 1.2 orders of magnitude with acceptable accuracy, reproducibility and recovery (98.16-100.50%). The limits of detection and quantification for 1 and 2 were 1.36, 4.11 and 1.11, 3.35 µg/mL respectively. The method is simple, accurate, precise and selective and may be routinely used for the quality control analysis of whole plant extract of O. burmannii for these two glycolipids.

Formulation and evaluation of HPMC topical gel of Ectoine

“…A developed green method, established on polar RP-HPLC was used for quantifying and screening Ectoine from gel formulae prepared according to ICH guidelines and Chinese Pharmacopoeia (22) . HPLC system model 1100 (Agilent Technologies, USA), with aquaternary pump, an autosampler, a variable wavelength detector and a chromatographic column of HiQsil C18 HS column (5µm particle size, 250 x 4.6mm) were used.…”

Bioprospecting of Marine Macrophytes Using MS-Based Lipidomics as a New Approach

“…HILIC coupled to MS was successfully applied to decode the lipidome of the red macroalgae Chondrus crispus [ 37 ] and the green macroalgae Codium tomentosum [ 68 ], among other red and brown macroalgae [ 107 ]. Although HILIC is increasing in popularity, RPLC is still widely used in lipidomics, namely, in plant lipidomics to separate GLs [ 80 , 117 , 118 , 119 , 120 ]. Kendal et al [ 50 ] used a C18 column to show which GL species could be obtained from Ulva armaricana and Solieria chordalis , possessing anti-proliferative properties against lung tumor.…”

“…This study enhances the use of the MS-base profiling of polar lipids towards the classification of marine organisms and, in general, the classification of complex lipid matrices. Although HILIC has become a relevant technique in this field, RPLC is still widely used in lipidomics, namely, in plant lipidomics to separate GLs [ 80 , 117 , 118 , 119 , 120 ]. Kendal et al [ 50 ] used a C18 column to discover which glycolipid species obtained from Ulva armaricana and Solieria chordalis displayed anti-proliferative properties against lung tumor.…”