Reverse transcriptase polymerase chain reaction (RT‐PCR) detection of HLP gene expression in Candida glabrata and its possible role in in vitro haemolysin production

Luo, Gang; Samaranayake, LP; Cheung, Bmy; Tang, Guoping

doi:10.1111/j.1600-0463.2004.apm11204-0509.x

Cited by 35 publications

(37 citation statements)

References 20 publications

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“…The possibility that species-specific hemolysins may exist should still be considered. These hemolysins may vary in molecular size and, thus, have different diffusion rates [20,36].…”

Section: Discussionmentioning

confidence: 99%

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Investigating Biofilm Production, Coagulase and Hemolytic Activity in Candida Species Isolated From Denture Stomatitis Patients

Yiğit

Aktaş

Dağistan

et al. 2011

EAJM

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Objective: Oral candidiasis, in the form of Candida-associated denture stomatitis, represents a common disease in a large percentage of denture wearers, and Candida albicans remains the most commonly isolated species. In this study, we aimed to evaluate biofi lm production, coagulase and hemolytic activity of Candida species isolated from denture stomatitis patients. Materials and Methods:This study included 70 patients (31 female, 39 male). Forty-eight of the patients were found to have a positive culture. A total of 48 Candida isolates representing fi ve species, C. albicans (n=17), C. glabrata (n=10), C. krusei (n=9), C. kefyr (n=7) and C. parapsilosis (n=5), were tested. Their coagulase activities were evaluated by a classical tube coagulase test with rabbit plasma. A blood plate assay on 3% enriched sheep blood Sabouraud-dextrose agar (SDA) was used to determine their in vitro hemolytic activities. Biofi lm production was determined by a visual tube method.Results: Twenty-one Candida isolates exhibited coagulase activity, and the coagulase activities of the C. albicans (64.7%) isolates were higher than other species. C. albicans, C. glabrata, C. kefyr and C. krusei species demonstrated beta hemolysis. C. parapsilosis strains failed to demonstrate any hemolytic activities. Fifteen (88.0%) of the C. albicans strains were biofi lm positive. Six (35.2%) of these strains were strongly positive, 8 (47.0%) C. albicans strains were moderately positive and 1 (5.8%) C. albicans strain was weakly positive. Sixteen (51.6%) of the non-albicans Candida strains were biofi lm positive while 15 (48.3%) did not produce biofi lms. Conclusion:The results of this present study indicate coagulase, hemolytic activity and biofi lm production by Candida spp. isolated from patients with denture stomatitis. Investigations of these virulence factors might be helpful in gaining information about the possible virulence of oral Candida species related to denture stomatitis.

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“…The possibility that species-specific hemolysins may exist should still be considered. These hemolysins may vary in molecular size and, thus, have different diffusion rates [20,36].…”

Section: Discussionmentioning

confidence: 99%

“…Hemolysins are known to be putative virulence factors contributing to Candida pathogenesis, particularly facilitating hyphal invasion in disseminated candidiasis [36]. The [37].…”

Section: Discussionmentioning

confidence: 99%

Investigating Biofilm Production, Coagulase and Hemolytic Activity in Candida Species Isolated From Denture Stomatitis Patients

Yiğit

Aktaş

Dağistan

et al. 2011

EAJM

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“…virulence. Hemolytic activity significantly contributes to the pathogenesis of disseminated candidiasis, especially facilitating hyphal penetration (Luo et al, 2004; Tsang et al, 2007), because hemolytic factors result in the release of hemoglobin from the erythrocytes of the host for later use as an iron source (Giolo and Svidzinski, 2010). …”

Section: Introductionmentioning

confidence: 99%

Evaluation of Virulence Factors In vitro, Resistance to Osmotic Stress and Antifungal Susceptibility of Candida tropicalis Isolated from the Coastal Environment of Northeast Brazil

et al. 2016

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Several studies have been developed regarding human health risks associated with the recreational use of beaches contaminated with domestic sewage. These wastes contain various micro-organisms, including Candida tropicalis. In this context, the objective of this study was to characterize C. tropicalis isolates from the sandy beach of Ponta Negra, Natal, Rio Grande do Norte, Brazil, regarding the expression of in vitro virulence factors, adaptation to osmotic stress and susceptibility to antifungal drugs. We analyzed 62 environmental isolates and observed a great variation among them for the various virulence factors evaluated. In general, environmental isolates were more adherent to human buccal epithelial cells (HBEC) than C. tropicalis ATCC13803 reference strain, and they also showed increased biofilm production. Most of the isolates presented wrinkled phenotypes on Spider medium (34 isolates, 54.8%). The majority of the isolates also showed higher proteinase production than control strains, but low phospholipase activity. In addition, 35 isolates (56.4%) had high hemolytic activity (hemolysis index > 0.55). With regard to C. tropicalis resistance to osmotic stress, 85.4% of the isolates were able to grow in a liquid medium containing 15% sodium chloride. The strains were highly resistant to the azoles tested (fluconazole, voriconazole and itraconazole). Fifteen strains were resistant to the three azoles tested (24.2%). Some strains were also resistant to amphotericin B (14 isolates; 22.6%), while all of them were susceptible for the echinocandins tested, except for a single strain of intermediate susceptibility to micafungin. Our results demonstrate that C. tropicalis isolated from the sand can fully express virulence attributes and showed a high persistence capacity on the coastal environment; in addition of showing high minimal inhibitory concentrations to several antifungal drugs used in current clinical practice, demonstrating that environmental isolates may have pathogenic potential.

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“…A PPT aumentada e policitemia relativa são características de uma desidratação (Feldman et al, 2000). Segundo alguns autores, as leveduras podem causar destruição de eritrócitos pela produção de hemolisinas (LUO et al, 2004;GIOLO et al, 2010), sendo que Candida spp. pode causar hemólise parcial e/ou total (LUO et al, 2004;YIGIT et al, 2009 (COLOMBO e GUIMARÃES, 2007).…”

Section: Methodsunclassified

Valor Diagnóstico Do Hemograma, Urocultura E Hemocultura Na Candidose Experimental Sistêmica

Matos¹,

Giordani²,

Guterres³

et al. 2014

AVS

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RESUMO:O presente estudo avaliou hemograma, proteínas plasmáticas totais (PPT), hemocultura e urocultura de ratos Wistar com candidose experimental sistêmica. Os animais (n=12) foram inoculados com Candida albicans e acompanhados por 30 dias. A coleta de sangue e urina foi feita durante as necropsias. No eritrograma, 11,1% apresentaram anemia e 44,4% policitemia, enquanto que as PPT estavam aumentadas em 11,1% dos ratos. Em relação ao leucograma, 88,9% apresentaram leucopenia por neutropenia e linfopenia. Os neutrófilos segmentados tiveram um aumento dos valores relativos (28-48%) em 66,7% dos animais quando comparados aos outros do grupo experimental (8-15%). A cultura da urina apresentou-se positiva em 33% das amostras e a do sangue em 25%, sendo que somente um animal com candidúria não apresentou hemocultura positiva. Os resultados demonstram que o hemograma completo, a urucultura e a hemocultura apresentaram-se de pouco valor no diagnóstico específico de candidose sistêmica experimental. Análises seriadas podem aumentar as chances de uro-hemoculturas positivas, e assim possibilitar a instituição de terapêutica precoce que possa beneficiar o paciente, e assim melhorar o prognóstico para a enfermidade. Palavras-chave: avaliação laboratorial; Candida albicans; micose; ratos Wistar DIAGNOSTIC VALUE OF BLOOD COUNT, URINE AND BLOOD CULTURES IN EXPERIMENTAL SYSTEMIC CANDIDIASISABSTRACT: This study evaluated blood count, total plasma protein (TPP), blood culture and urine of Wistar rats (n=12) with experimental systemic candidiasis. The animals were inoculated with Candida albicans and monitored for 30 days. The collection of blood and urine was made during the autopsy. In red blood cell, was observed anemic in 11.1% and polycythemia in 44.4%, while the PPT were increased in 11.1% of rats. In the WBC, 88.9% had severe neutropenia and lymphopenia. The segmented neutrophils had increase in relative values (28-48%) in 66.7% of animals when compared to other experimental group (8-15%). The urine culture were positive in 33% and blood samples in 25%, only one animal with candida on urine had no positive blood cultures. The results demonstrate that the full blood count, the culture of blood and the culture of urine showed little value in specific diagnosis of experimental systemic candidiasis. Serial analyzes may increase the chances of positive blood and urine cultures, and thus enable the institution of early treatment that can benefit the patient, and thus improve prognosis.

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Reverse transcriptase polymerase chain reaction (RT‐PCR) detection of HLP gene expression in Candida glabrata and its possible role in in vitro haemolysin production

Cited by 35 publications

References 20 publications

Investigating Biofilm Production, Coagulase and Hemolytic Activity in Candida Species Isolated From Denture Stomatitis Patients

Investigating Biofilm Production, Coagulase and Hemolytic Activity in Candida Species Isolated From Denture Stomatitis Patients

Evaluation of Virulence Factors In vitro, Resistance to Osmotic Stress and Antifungal Susceptibility of Candida tropicalis Isolated from the Coastal Environment of Northeast Brazil

Valor Diagnóstico Do Hemograma, Urocultura E Hemocultura Na Candidose Experimental Sistêmica

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