Reversed-phase high-performance liquid chromatography of amphoteric β-lactam antibiotics: effects of columns, ion-pairing reagents and mobile phase pH on their retention times

Huang, Hong-Shian; Wu, Jing-Ran; Chen, Mei‐Ling

doi:10.1016/0378-4347(91)80081-m

Cited by 27 publications

(3 citation statements)

References 10 publications

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“…In the present work a solid phase extraction technique was employed to overcome the problem of mixed polarity of cefaclor, which makes the solvent extraction procedure insufficient (14). Reported cephalosporin analytical methods for biological samples have predominantly relied on protein precipitation with cold methanol and 0·1 M sodium acetate or with methanol alone prior to HPLC separation using an RP C 18 column (14–17) and spectrophotometric determination (18–20).…”

Section: Discussionmentioning

confidence: 99%

Bioequivalence evaluation of two brands of cefaclor 500 mg capsules: quantification of cefaclor using solid phase extraction technique

Tutunji¹,

Jarrar²,

Musameh³

et al. 2001

J Clin Pharm Ther

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Section: Discussionmentioning

confidence: 99%

Bioequivalence evaluation of two brands of cefaclor 500 mg capsules: quantification of cefaclor using solid phase extraction technique

Tutunji¹,

Jarrar²,

Musameh³

et al. 2001

J Clin Pharm Ther

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“…We found that all three factors had a significant effect on the resolution (Supplementary Materials). The optimization of HPLC methods often involves the evaluation of pH [23][24][25][26][27] and temperature [23,[28][29][30][31]. The temperature affects viscosity [28] as well as hydrophobic interactions by influencing enthalpy and entropy [32,33].…”

Section: Experimental Design For Intermediate Purificationmentioning

confidence: 99%

Isolation and Purification of a Hydrophobic Non-Ribosomal Peptide from an Escherichia coli Fermentation Broth

et al. 2021

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Non-ribosomal peptide synthases (NRPSs) generate versatile bioactive peptides by incorporating non-proteinogenic amino acids and catalyzing diverse modifications. Here, we developed an efficient downstream process for the capture, intermediate purification and polishing of a rhabdopeptide (RXP) produced by the NRPS VietABC. Many typical unit operations were unsuitable due to the similar physical and chemical properties of the RXP and related byproducts. However, we were able to capture the RXP from a fermentation broth using a hydrophobic resin (XAD-16N), resulting in a 14-fold increase in concentration while removing salts as well as polar and weak non-polar impurities. We then used ultra-high-performance liquid chromatography (UHPLC) for intermediate purification, with optimized parameters determined using statistical experimental designs, resulting in the complete removal of hydrophobic impurities. Finally, the UHPLC eluents were removed by evaporation. Our three-step downstream process achieved an overall product recovery of 81.7 ± 8.4%.

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“…Therefore, the molecule has an anionic charge. Amoxicillin and ampicillin have amino groups whose pK a values are between 7.2 and 7.4, and amoxicillin has a phenolic substitution leading to a third pK a of 9.6 [32]. In these cases, the anionic charge coexists with the cationic charge of the amino group in the zwitterionic form of the molecule and this form is in equilibrium with the neutral one as the amino group is protonated until the pH medium reaches the second pK a .…”

Section: Separations With Phosphate Buffermentioning

confidence: 99%

Sample stacking for the analysis of eight penicillin antibiotics by micellar electrokinetic capillary chromatography

et al. 2005

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We studied the use of micellar electrokinetic capillary chromatography for separating eight penicillins. The method consists of (i) an electrophoretic separation based on micellar electrokinetic capillary chromatography, which uses sodium dodecyl sulfate (SDS) as surfactant; (ii) a sample stacking technique called reverse electrode polarity stacking mode (REPSM); and (iii) direct UV detection. The background electrolyte that gave complete separation contained 20 mM sodium borate buffer and 60 mM SDS. The sensitivity of the method was improved by an enrichment step that used on-column stacking. The limits of detection were at the microg.L(-1) level for the penicillins and did not detract from the peak resolution.

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Reversed-phase high-performance liquid chromatography of amphoteric β-lactam antibiotics: effects of columns, ion-pairing reagents and mobile phase pH on their retention times

Cited by 27 publications

References 10 publications

Bioequivalence evaluation of two brands of cefaclor 500 mg capsules: quantification of cefaclor using solid phase extraction technique

Bioequivalence evaluation of two brands of cefaclor 500 mg capsules: quantification of cefaclor using solid phase extraction technique

Isolation and Purification of a Hydrophobic Non-Ribosomal Peptide from an Escherichia coli Fermentation Broth

Sample stacking for the analysis of eight penicillin antibiotics by micellar electrokinetic capillary chromatography

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