Reversible Aptamer-Au Plasmon Rulers for Secreted Single Molecules

Lee, Somin Eunice; Chen, Qian; Bhat, Ramray; Petkiewicz, Shayne; Smith, Jessica M.; Ferry, Vivian E.; Correia, Ana Luísa; Alivisatos, A. Paul; Bissell, Mina J.

doi:10.1021/acs.nanolett.5b01161

Cited by 101 publications

(117 citation statements)

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“…Among various noble metal nanomaterials, gold nanomaterials are most intensely investigated as imaging contrast agents and nanocarriers . As a result, the integration of aptamer with gold nanomaterials, such as Au nanorods (AuNRs), Au nanoparticles, Au nanocages, Au nanostars (AuNSs), hollow gold nanospheres (HAuNS), and Au nanoclusters (Au NCs), has been widely employed in in vivo applications in diverse areas, such as intracellular molecular imaging, cancer cell and tumor imaging, targeted delivery of therapeutic cargos (e.g., anticancer drug, protein, and gene), and cancer therapy . In one example, Huang et al demonstrated the use of AuNRs as an efficient and robust multivalent platform for molecular assembly of aptamers for targeted cell imaging .…”

Section: Molecular Engineering Of Aptamer‐based Nanomaterials Toward mentioning

confidence: 99%

Molecular Engineering of Functional Nucleic Acid Nanomaterials toward In Vivo Applications

Zhang

Lan

2019

Adv Healthcare Materials

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equally exciting and perhaps more challenging field of application is toward their in vivo applications, including living animals and human bodies, in diverse areas, [2] such as sensing, drug delivery, medical imaging, and cancer therapy. However, most of these nanomaterials lack selectivity toward targets of interests. Therefore, to employ these nanomaterials for a wider range of in vivo applications, various molecular engineering approaches have been employed to obtained nanomaterials functionalized with biomolecules to enable selective targeting. [3][4][5] Among these biomolecules, functional nucleic acids, or FNAs, have shown the most promise.FNAs are DNA or RNA molecules that can interact with or bind to a specific analyte, resulting in a conformational change and/or a catalytic reaction. [6] As their names suggested, ribozymes and deoxyribozymes (called DNAzyme hereafter) can act as enzymes in the presence of cofactors to catalyze various reactions, including cleavage and ligation of RNA/DNA, and phosphorylation and peroxidation of DNA. On the other hand, riboswitches and DNA aptamers are antibody-like receptors that can bind target molecules with high affinity and selectivity. Furthermore, the binding abilities of riboswitches and aptamers can be combined with the enzymatic function of ribozymes or DNAzymes to form aptazymes so that the binding of targets can inhibit or enhance the catalytic activities. These nucleic acids, including DNAzymes, aptamers, and aptazymes, are collectively known as FNAs to emphasize their functions beyond the traditional genetic storage and transformations roles for DNA and RNA. Unlike DNA and RNA inside biological systems, FNAs are isolated via a combinatorial technique known as in vitro selection, or a process also known as systematic evolution of ligands by exponential enrichment (SELEX). The discovery of new functions of nucleic acids has not only resulted in major advances in the fields of molecular biology and biochemistry, but also revolutionized sensors designs for both in vitro and in vivo applications. [7,8] Over the past decade, numerous FNA-based nanomaterials have been developed. [9][10][11][12] Among these FNA-based nanosystems, the functions of FNAs can be categorized into two types: diagnostic function and therapeutic function. For the diagnostic function, the FNAs serve either as the biorecognition ligands for direct sensing and imaging of the Recent advances in nanotechnology and engineering have generated many nanomaterials with unique physical and chemical properties. Over the past decade, numerous nanomaterials are introduced into many research areas, such as sensors for environmental monitoring, food safety, point-ofcare diagnostics, and as transducers for solar energy transfer. Meanwhile, functional nucleic acids (FNAs), including nucleic acid enzymes, aptamers, and aptazymes, have attracted major attention from the biomedical community due to their unique target recognition and catalytic properties. Benefiting from the recent progress of molecular engine...

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Section: Molecular Engineering Of Aptamer‐based Nanomaterials Toward mentioning

confidence: 99%

Molecular Engineering of Functional Nucleic Acid Nanomaterials toward In Vivo Applications

Zhang

Lan

2019

Adv Healthcare Materials

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“…Conceptually, nanoparticle dimers are the simplest examples of assembled structures of nanoparticles. They have been extensively studied as sensors or plasmonic rulers using absorbance or scattering spectroscopy . However, a key disadvantage of these spectroscopic methods is that they cannot effectively detect the interactions between the nanoparticles if the interparticle distance is too large or if the particles are too small in size.…”

Section: Introductionmentioning

confidence: 99%

“…They have been extensively studied as sensors or plasmonic rulers using absorbance or scattering spectroscopy. [10][11][12] However,akey disadvantage of theses pectroscopic methods is that they cannot effectively detect the interactions between the nanoparticles if the interparticle distance is too large or if the particlesa re too small in size. The localised surfacep lasmonr esonance is progressivelyd amped out for smallp articles (< 30 nm), which drastically decreases their light scattering response;s uch particles can be considered "weakly plasmonic".…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic and Hydrodynamic Characterisation of DNA‐Linked Gold Nanoparticle Dimers in Solution using Two‐Photon Photoluminescence

et al. 2018

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Two-photon photoluminescence (TPPL) emission spectra of DNA-gold nanoparticle (AuNP) monoconjugates and the corresponding DNA-linked AuNP dimers are obtained by photon time-of-flight spectroscopy. This technique is combined with two-photon photoluminescence fluctuation correlation spectroscopy (TPPL-FCS) to simultaneously monitor the optical and hydrodynamic behaviour of these nano-assemblies in solution, with single-particle sensitivity and microsecond temporal resolution. In this study, the AuNPs have an average core diameter of 12 nm, which renders their dark-field plasmonic light scattering too weak for single-particle imaging. Moreover, as a result of the lack of plasmonic coupling in the dimers, the optical extinction, scattering and photoluminescence spectra of the DNA-AuNP complexes are not sufficiently different to distinguish between monomers and dimers. The use of TPPL-FCS successfully addresses these bottlenecks and enables the distinction between AuNP monomers and AuNP dimers in solution by measurement of their hydrodynamic rotational and translational diffusion.

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“…2 Even more impressive is the application of this plasmon ruler toward the accurate detection and measurement of secreted single molecules in the cellular microenvironment. Understanding how cells communicate with their cellular microenvironment remains a challenge to study.…”

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confidence: 99%

Congratulations to the 2016 JALA Ten!

Chow

2016

SLAS Technology

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EditorialOn behalf of the JALA scientific advisors and JALA editorial board, I am happy to present this year's honorees of the prestigious JALA Ten. Each year, JALA seeks to highlight and honor the very best work of the year that will have a deep impact on how technology is used across a wide range of disciplines, including automation, life sciences and biomedical research, diagnostics, drug delivery, and regenerative medicine. Implementing the latest advances in microfluidics, nanotechnology, materials science, and other fields of research, this year's JALA Ten honorees have and will continue to change the way research is performed and the way diseases are diagnosed and treated. As such, the work highlighted here should have far-reaching impact in our everyday lives. It demonstrates the promise that science brings toward a better future.While a number of areas of research will feel the impact of this year's JALA Ten, one highlight of the collection is the diverse ways in which the honorees have advanced biological molecule detection and established foundations for tomorrow's biosensors in life sciences research and medical diagnostics.1-3 For example, Peter Lillehoj at Michigan State University (USA) and his collaborators have developed a microfluidic biosensor using immobilized antimicrobial peptides for highly specific, multiplexed detection of bacterial pathogens.1 Showing both high pathogen detection specificity and accurate pathogen quantification, this work is an example of how microfluidic technology is changing how medical professionals will track and diagnose infectious diseases.On the other end of the spectrum, Somin Eunice Lee at the University of Michigan (USA) has harnessed the power of nanotechnology to develop a gold nanoparticle-based plasmon ruler that is capable of single-molecule measurements.2 Even more impressive is the application of this plasmon ruler toward the accurate detection and measurement of secreted single molecules in the cellular microenvironment. Understanding how cells communicate with their cellular microenvironment remains a challenge to study. Tools such as Lee's plasmon ruler vastly improve life sciences researchers' abilities to learn more about the interplay between cells and their microenvironments.Another area of research highlighted this year is the advancement of technology toward customizable platforms for increased personalized clinical and research applications. [4][5][6][7] The development of tunable injectable microporous gel scaffolds by researchers at the University of California, Los Angeles (USA) not only brings truly personalized medicine to regenerative medicine, but also allows researchers to quickly optimize culture conditions in research and drug development applications that require complex three-dimensional (3D) cell cultures. 4 The development of integrated platforms composed of modular technologies increases the diversity of clinical and research applications to which technology such as microfluidics can be applied and is highlighted here by work f...

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Reversible Aptamer-Au Plasmon Rulers for Secreted Single Molecules

Cited by 101 publications

References 44 publications

Molecular Engineering of Functional Nucleic Acid Nanomaterials toward In Vivo Applications

Molecular Engineering of Functional Nucleic Acid Nanomaterials toward In Vivo Applications

Spectroscopic and Hydrodynamic Characterisation of DNA‐Linked Gold Nanoparticle Dimers in Solution using Two‐Photon Photoluminescence

Congratulations to the 2016 JALA Ten!

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