Reversible regulation of thrombin adsorption and desorption based on photoresponsive-aptamer modified gold nanoparticles

Yu, Jing; Yang, Liangrong; Liang, Xia; Dong, Tingting; Liu, Huizhou

doi:10.1016/j.talanta.2015.06.053

Cited by 4 publications

(3 citation statements)

References 34 publications

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“…13−15 The ability to incorporate molecular photoswitches into synthetic DNA sequences has opened up new possibilities for stimulus-responsive materials in these fields, 12,16−25 while at the same time enabling applications such as photoregulation of gene expression 26−28 and enzymatic reactions 29−31 and also facilitating studies of aptamer folding and target binding. 32,33 Perhaps the most widely used DNA photoswitch at present is based on azobenzene attached to the sugar−phosphate backbone during solid-phase DNA synthesis, as pioneered by Asanuma et al 34 When inserted into the phosphate backbone of a DNA via a D-threoninol linkage, the azobenzene intercalates with the neighboring nucleobase pairs through πstacking interactions and stabilizes the DNA duplex despite the distortion in the double helical structure. 35−37 Upon UV irradiation, trans-azobenzene converts to the nonplanar cis form, which destabilizes the duplex.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Nucleic acids are now extensively used to design, tailor, and assemble nanomaterials ranging from Mirkin-type spherical nucleic acids − to functional aptamers, − DNA origami, − and nanomachines. − The ability to incorporate molecular photoswitches into synthetic DNA sequences has opened up new possibilities for stimulus-responsive materials in these fields, ,− while at the same time enabling applications such as photoregulation of gene expression − and enzymatic reactions − and also facilitating studies of aptamer folding and target binding. , …”

Section: Introductionmentioning

confidence: 99%

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Temperature-Dependent Photoisomerization Quantum Yields for Azobenzene-Modified DNA

et al. 2017

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We study the photoisomerization quantum yield of azobenzene-modified DNA as a function of temperature for various DNA sequences. We find that even though the photoisomerization quantum yield of free azobenzene is essentially temperature-independent, the trans-to-cis photoisomerization quantum yield depends strongly on temperature when the azobenzene is incorporated into DNA. We show that this temperature dependence is DNA-sequence-dependent and closely linked to the melting temperature of the host DNA. While the trans-to-cis isomerization quantum yield is sequenceand temperature-dependent, in contrast, the thermal cis-totrans isomerization of azobenzene embedded in DNA is sequence-independent and exhibits Arrhenius-like behavior with an activation energy of 88.8 ± 0.693 kJ/mol and a smaller pre-exponential factor than free azobenzene, yielding first-order cis-to-trans kinetics with a rate constant of (2.08 ± 0.00952) × 10 −6 s −1 at 25 °C. These results provide an understanding of the azobenzene isomerization mechanisms in DNA sequences to enable more efficient design of optically reprogrammable nanomaterials and biosensors.

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Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Temperature-Dependent Photoisomerization Quantum Yields for Azobenzene-Modified DNA

et al. 2017

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“…Azobenzene is an ideal photoresponsive molecule that is characteristic of trans -to- cis conversion upon irradiation with particular wavelengths of light (i.e., photoisomerization). Ultraviolet light, whose energy is comparable to the energy gap of the π–π* transition, can evoke trans -to- cis conversion of azobenzene, whereas blue light with energy equivalent to the n−π* transition is able to help its reverse cis -to- trans isomerization. − As azobenzene is introduced into the paired bases of a DNA chain, the DNA duplex becomes photoresponsive. Very interestingly, visible-light irradiation has an insignificant impact on the chemical structure of DNA duplexes if azobenzene is in a trans form (a planar molecule), whereas the UV irradiation on trans -azobenzene can cause trans -to- cis conversion; then, the duplex would dissociate.…”

mentioning

confidence: 99%

Photoinduced Regeneration of an Aptamer-Based Electrochemical Sensor for Sensitively Detecting Adenosine Triphosphate

et al. 2018

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Electrochemical aptasensors generally include three elements, that is, recognition element, signal-transformation element, and regeneration element. In this study, a new adenosine triphosphate (ATP) aptasensor is developed by combining three elements into one DNA oligonucleotide chain. In the DNA oligonucleotide chain, DNA aptamer is used as the recognition element, ferrocene group attached at the 3'-end of the aptamer is used as the signal-transformation element, and azobenzene moiety embedded into the DNA chain is used as the regeneration element. In addition to the similar analytical properties with the traditional ones, the aptasensor developed here is easily regenerated with UV-light irradiation. The current response recorded on the aptasensor increases with increasing the concentration of ATP in the incubation solution and is linear with the logarithm of ATP concentration in the range from 1 nM to 100 μM. The limit of detection is 0.5 nM (S/N = 3). The basal level of ATP in the rat brain cortex microdialysate is determined to be 21.33 ± 4.1 nM ( n = 3). After being challenged with ATP, the aptasensor could be readily regenerated by UV-light irradiation for more than seven cycles. The regeneration of the aptasensor is proposed to be regulated by conversing azobenzene from its trans to cis form under UV irradiation.

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Photoreversible DNA nanoswitch-based eluent-free strategy for the direct and effective isolation of highly-active thrombin from whole blood

Lin

Xing

et al. 2023

International Journal of Biological Macromolecules

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Reversible regulation of thrombin adsorption and desorption based on photoresponsive-aptamer modified gold nanoparticles

Cited by 4 publications

References 34 publications

Temperature-Dependent Photoisomerization Quantum Yields for Azobenzene-Modified DNA

Temperature-Dependent Photoisomerization Quantum Yields for Azobenzene-Modified DNA

Photoinduced Regeneration of an Aptamer-Based Electrochemical Sensor for Sensitively Detecting Adenosine Triphosphate

Photoreversible DNA nanoswitch-based eluent-free strategy for the direct and effective isolation of highly-active thrombin from whole blood

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