Reversing Age Related Changes of the Laryngeal Muscles by Chronic Electrostimulation of the Recurrent Laryngeal Nerve

Karbiener, Michael; Jarvis, Jonathan C.; Perkins, Justin; Lanmüller, Hermann; Schmoll, Martin; Rode, Hanna S.; Gerstenberger, Claus; Gugatschka, Markus

doi:10.1371/journal.pone.0167367

Cited by 23 publications

(26 citation statements)

References 38 publications

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“…Once the IPG has been programmed, no further anesthesia or sedation was necessary. As in our previous study, the very first training session of awake animals was carefully monitored, and no signs of stress could be observed. During every other week, we performed a transnasal endoscopy in the sedated but awake animal to ensure the implant was functioning correctly by observing VF movement.…”

Section: Methodsmentioning

confidence: 91%

“…Recently, we demonstrated a new approach to reverse the effects of laryngeal muscular ageing by FES . Electrical stimulation was delivered by a cuff electrode that was coiled around the RLN.…”

Section: Discussionmentioning

confidence: 99%

“…Six larynges were examined for gene expression and six for morphometric analyses. Animal procedures were carried out using the same methods as described previously . We obtained an approval from the Austrian Federal Ministry of Science, Research and Economy (approval number: BMWFW‐66.010/0039‐WF/V/3b/2015).…”

Section: Methodsmentioning

confidence: 99%

“…A sample from the central region (i.e., the part of the TAM underlying the VF, between the anterior commissure and the vocal process) was prepared, snap frozen in liquid nitrogen, and stored at −80 °C. RNA isolation, determination of RNA concentration and quality, reverse‐transcription and quantitative real‐time polymerase chain reaction (RT‐qPCR) were performed as previously described . Primer sequences are provided in Table .…”

Section: Methodsmentioning

confidence: 99%

“…Animal procedures were carried out using the same methods as described previously. 8 We obtained an approval from the Austrian Federal Ministry of Science, Research and Economy (approval number: BMWFW-66.010/0039-WF/V/3b/2015). All procedures complied with the institution's animal care guidelines and were carried out by experienced veterinarians.…”

Section: Surgical Procedures and Trainingmentioning

confidence: 99%

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Functional Electrical Stimulation Leads to Increased Volume of the Aged Thyroarytenoid Muscle

et al. 2018

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Objectives/Hypothesis To reverse sarcopenia and increase the volumes of atrophied laryngeal muscles by functional electrical stimulation (FES) using a minimal invasive surgical procedure in an aged ovine model. Study Design Prospective animal study. Methods A stimulation electrode was placed unilaterally near the terminal adduction branch of the recurrent laryngeal nerve (RLN) adjacent to the right cricothyroid joint. The electrode was connected to an implant located subcutaneously at the neck region. Predesigned training patterns were automatically delivered by a bidirectional radio frequency link using a programming device and were repeated automatically by the implant every other day over 11 weeks in the awake animal. Outcome parameters comprised volumetric measurements based on three‐dimensional reconstructions of the entire thyroarytenoid muscle (TAM), as well as gene expression analyses. Results We found significant increases of the volumes of the stimulated TAM of 11% and the TAM diameter at the midmembranous parts of the vocal folds of nearly 40%. Based on gene expression, we did not detect a shift of muscle fiber composition. Conclusions FES of the terminal branches of the RLN is a secure and effective way to reverse the effects of age‐related TAM atrophy and to increase volumes of atrophied muscles. Level of Evidence NA Laryngoscope , 128:2852–2857, 2018

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Section: Methodsmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Surgical Procedures and Trainingmentioning

confidence: 99%

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Functional Electrical Stimulation Leads to Increased Volume of the Aged Thyroarytenoid Muscle

et al. 2018

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Ultrasound Elastography Detects Age‐Related Changes in Adult False Vocal Folds

Chandra

Ortíz

Weitzel

et al. 2022

J of Ultrasound Medicine

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Purpose To investigate the feasibility of ultrasound elastography for assessing the symmetry in stiffness values and movements of both false vocal folds (FVFs). Methods After Institutional Review Board's approval and written informed consent obtained, we measured ultrasound strain and shear wave velocity (SWV) of the bilateral FVF in vocal fold abduction and adduction in 30 participants using a linear array transducer (4–10 MHz). Twenty‐eight participants met inclusion criteria as healthy subjects for analysis. Mean strain of FVF produced by FVF movement from abduction to adduction was analyzed using 2D speckle‐tracking software offline. A SWV ratio ([SWVadduction – SWabduction]/SWabduction) was developed to test the contractibility of FVF. Statistical analysis included one‐way ANOVA to test the difference in mean strain, SWV (adduction and abduction), and SWV ratio among the three age groups; linear regression to analyze correlations of stiffness and movement between the right and left FVF; and intraclass correlation coefficient (ICC) to examine intra‐ and interobserver reliability in performing shear wave elastography (SWE) of FVF. Results The 28 healthy participants were divided into 3 age groups (10 of young 20–44 years; 9 of mid‐age 45–64 years; and 9 of senior ≥65 years). The SWV in FVF abduction was higher and the SWV ratio was lower in seniors compared to young participants (P < .05). Good to excellent correlation of mean strain and SWV between both FVFs (R2 > 0.89). The reliability of performing SWE of FVF was moderate to excellent. Conclusion Ultrasound elastography is feasible to assess the stiffness, dynamic movement, and symmetry of adult FVF, and healthy seniors may exhibit increased FVF stiffness.

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Functional Electrical Stimulation for Presbyphonia: A Prospective Randomized Trial

Gugatschka

Feiner

Mayr³

et al. 2020

The Laryngoscope

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The aim of this prospective study was to examine the effects of transcutaneous functional electrical stimulation (FES) in a group of elderly women with presbyphonia. Study Design: Prospective randomized study. Methods: Fourteen participants were enrolled prospectively and attributed randomly to two different treatment groups, where one group (n = 7) received 8 weeks of training (5 days a week), whereas the other group (n = 7) received 4 weeks of ineffective stimulation, followed by 4 weeks of effective training. Stimulation protocols were established during baseline examination and confirmed with endoscopy to ensure a glottal reaction. Numerous acoustical, vocal, patient-centered, and respiratory parameters were obtained at several time points. Results: Neither 4 weeks nor 8 weeks of functional electrical transcutaneous stimulation led to changes of vocal, acoustical, or respiratory parameters, apart from patient-centered items (Voice Handicap Index 12, Voice-Related Quality of Life), which improved over time. However, there were no differences between the two arms for both items. Conclusions: Transcutaneous FES over 4 weeks and 8 weeks did not lead to significantly improved objective voice and acoustical parameters, which could be caused by the fact that the muscles of interest cannot be targeted specifically enough. However, we found a significant improvement of subjective voice perception and voice-related quality of life in both groups. We explain this finding with an observer-expectancy effect secondary to the very time-consuming and elaborate study procedures.

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Reversing Age Related Changes of the Laryngeal Muscles by Chronic Electrostimulation of the Recurrent Laryngeal Nerve

Cited by 23 publications

References 38 publications

Functional Electrical Stimulation Leads to Increased Volume of the Aged Thyroarytenoid Muscle

Functional Electrical Stimulation Leads to Increased Volume of the Aged Thyroarytenoid Muscle

Ultrasound Elastography Detects Age‐Related Changes in Adult False Vocal Folds

Functional Electrical Stimulation for Presbyphonia: A Prospective Randomized Trial

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