Reversing effect of Lupeol on vasculogenic mimicry in murine melanoma progression

Bhattacharyya, Sayantan; Mitra, Debolina; Ray, Sudipta; Biswas, Nirjhar; Banerjee, Samir; Majumder, Biswanath; Mustafi, Saunak Mitra; Murmu, Nabendu

doi:10.1016/j.mvr.2018.10.008

Cited by 26 publications

(19 citation statements)

References 51 publications

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“…Vasculogenic mimicry is known as nonendothelialized vessel-like channels in malignant tumors which could be dependent on to supply blood for tumor growth [19]. As stated in previous reports, vasculogenic mimicry is closely related with metastasis and poor prognosis in many tumors such as melanoma [20, 21], glioma [22, 23], breast cancer [24], and hepatic carcinoma [25]. Vasculogenic mimicry was considered to be associating with epithelial-mesenchymal transition [26] and as a marker of poor prognosis in prostate cancer [27].…”

Section: Discussionmentioning

confidence: 99%

Kaempferol Promotes Apoptosis While Inhibiting Cell Proliferation via Androgen-Dependent Pathway and Suppressing Vasculogenic Mimicry and Invasion in Prostate Cancer

Jiang

Wang

et al. 2019

Analytical Cellular Pathology

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Kaempferol is a well-known natural flavonol reported to be a potential treatment for multiple cancers. In this study, we demonstrated that cell growth of androgen-sensitive LNCaP cells could be inhibited 33% by 5 μM kaempferol, around 60% by 10 μM kaempferol, and almost 100% by 15 μM kaempferol. Also, kaempferol showed relatively limited effect on PC-3 cells and nonmalignant RWPE-1 cells. In the presence of DHT, the IC50 for kaempferol was 28.8 ± 1.5 μM in LNCaP cells, 58.3 ± 3.5 μM in PC-3 cells, and 69.1 ± 1.2 μM in RWPE-1 cells, respectively. Kaempferol promotes apoptosis of LNCaP cells in a dose-dependent manner in the presence of dihydrotestosterone (DHT). Then, luciferase assay data showed that kaempferol could inhibit the activation of androgen receptors induced by DHT significantly. The downstream targets of androgen receptors, such as PSA, TMPRSS2, and TMEPA1, were found decreased in the presence of kaempferol in qPCR data. It was then confirmed that the protein level of PSA was decreased. Kaempferol inhibits AR protein expression and nuclear accumulation. Kaempferol suppressed vasculogenic mimicry of PC-3 cells in an in vitro study. In conclusion, kaempferol is a promising therapeutic candidate for treatment of prostate cancer, where the androgen signaling pathway as well as vasculogenic mimicry are involved.

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Section: Discussionmentioning

confidence: 99%

Kaempferol Promotes Apoptosis While Inhibiting Cell Proliferation via Androgen-Dependent Pathway and Suppressing Vasculogenic Mimicry and Invasion in Prostate Cancer

Jiang

Wang

et al. 2019

Analytical Cellular Pathology

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“…B16-F10 cells were seeded at a very low number (500 cells in 2 mL/well) in six-well plates. After 24 h, they were treated with the optimum concentration of PCFE (100 µ g/mL) alone and in combination with dacarbazine at 1000 µ g/mL [ 37 ] and incubated for 72 hours. The colonies were fixed with methanol and stained with Harry's hematoxylin.…”

Section: Methodsmentioning

confidence: 99%

“…In order to check the effect of dacarbazine alone and in combination with PCFE in solid melanoma tumor, the experiment was designed in 5 groups. The first group was treated with dacarbazine at 80 mg/kg body weight [ 37 ]; second, third, and fourth groups were treated with different doses of PCFE 100, 150, and 200 mg/kg b.w., respectively, along with dacarbazine, and the fifth group was considered as a control. The treatment was carried out over a period of seven days; on the 8th day, all animals were sacrificed.…”

Section: Methodsmentioning

confidence: 99%

Botanical from Piper capense Fruit Can Help to Combat the Melanoma as Demonstrated by In Vitro and In Vivo Studies

Wamba

Ghosh

Mbaveng

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Piper capense belongs to Piperaceae family and has long been used as a traditional medicine to treat various diseases in several parts of Africa. The present study aims to investigate the effect of Piper capense fruit extract (PCFE) alone and in combination with dacarbazine on metastatic melanoma cell line B16-F10 and in vivo in C57BL/6J mice. Cytotoxic effects of PCFE alone and in association with dacarbazine on B16-F10 cells were studied by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay and colony formation assay. Wound healing assay, immunofluorescence staining, and western blot analysis were performed to evaluate the individual and combined effect of PCFE and dacarbazine on epithelial-mesenchymal transition (EMT). For in vivo studies, C57BL/6J mice were subcutaneously injected with B16-F10 cells (5 × 105 cells/mL), and the effect of PCFE and dacarbazine was studied on tumor development. The alteration of EMT was evaluated by targeting E-cadherin, vimentin, and CD133 in PCFE alone and in combination with dacarbazine-treated tumor tissues by western blot analysis. Phytochemical screening of PCFE reveals the presence of certain secondary metabolites. Our results showed that PCFE alone and in association with dacarbazine has a good activity in preventing B16-F10 melanoma cell progression and clonogenicity. This extract also regulated EMT. In vivo results showed that PCFE (100 mg/kg body weight) reduced tumor size in C57BL/6J mice along with the decrease in the expression of vasculogenic mimicry (VM) tubes as well as an improvement in the qualitative and quantitative expression of markers involved in EMT. Our study suggests that PCFE may be useful for managing the growth and metastasis of melanoma.

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“…Lupeol can become a more power full anticancer agent that treated the B16-F10 cell line and also inhibit the vasculogenic mimicry with inducing Dacarbazine drug resistance. 15 Lung cancer is treated with the exploration and evaluation of new molecules of lupeol. Lupeol effects on lung cancer A427 cancer cells and normal MRC-5 cells.…”

Section: Lupeol Act As Anti-cancerousmentioning

confidence: 99%

A Review on Pharmacological Activities of Lupeol and its Triterpene Derivatives

Sharma¹,

Palia²,

Chaudhary³

et al. 2020

J. Drug Delivery Ther.

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Lupeol is a pentacyclic triterpenoid commonly distributed in the plant kingdom and is found in edible fruits and vegetables. It is a naturally occurring triterpene that is used to reduce the inflammatory responses and also have immunomodulating properties. Lupeol and its derivatives have a great potential to act as an anti- inﬂammatory, anti-microbial, anti-proliferative, anti-invasive, anti-angiogenic, anti-protozoal, and cholesterol-lowering agent. Various studies have shown that anti-inflammatory activity of lupeol through the modulation of p-38 pathways inhibits neuroinflammation in the cerebellum and induces neuroprotection. It has been also found effective on lung cancer (i.e A427 cancer cells and normal MRC-5 cells). Observation of inhibiting the growth of lung cancer cells is checked by MTT assay. Lupeol and its ester lupeol linoleate have been used to reduce the levels of hypercholesterolemia in the rats and decrease the activities of such enzymes namely Na+, K+-ATPase, Ca2+-ATPase, and Mg2+. Lupeol also decreases the levels of calcium-oxalate and has cytoprotective action against free-radical-induced damage and also decreases the level of cadmium in the kidney. Keywords: Lupeol, anti- inﬂammatory, anti-protozoal, triterpenoids

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Reversing effect of Lupeol on vasculogenic mimicry in murine melanoma progression

Cited by 26 publications

References 51 publications

Kaempferol Promotes Apoptosis While Inhibiting Cell Proliferation via Androgen-Dependent Pathway and Suppressing Vasculogenic Mimicry and Invasion in Prostate Cancer

Kaempferol Promotes Apoptosis While Inhibiting Cell Proliferation via Androgen-Dependent Pathway and Suppressing Vasculogenic Mimicry and Invasion in Prostate Cancer

Botanical from Piper capense Fruit Can Help to Combat the Melanoma as Demonstrated by In Vitro and In Vivo Studies

A Review on Pharmacological Activities of Lupeol and its Triterpene Derivatives

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