Reversing Effect of Ring Finger Protein 43 Inhibition on Malignant Phenotypes of human Hepatocellular Carcinoma

Abstract

It has been shown that Ring finger protein 43 (RNF43) is overexpressed in colorectal cancer and mediates cancer cell proliferation; however, its role in hepatocellular carcinoma (HCC) remains unknown. In this study, we found that RNF43 was frequently overexpressed in HCCs, and this overexpression was correlated with positive vascular invasion, poor tumor differentiation, and advanced tumor stage. Functional studies showed that knockdown of RNF43 could induce apoptosis and inhibit proliferation, invasion, colony formation, and xenograft growth of HCCs. Microarray-based gene profiling showed a total of 229 genes differentially expressed after RNF43 knockdown, many of which are involved in oncogenic processes such as cell proliferation, cell adhesion, cell motility, cell death, DNA repair, and so on. These results suggest that RNF43 is involved in tumorigenesis and progression of HCCs and that antagonism of RNF43 may be beneficial for HCC treatment.

Molecular Role of RNF43 in Canonical and Noncanonical Wnt Signaling

“…In addition, several reports (33,34,48,49) and the COSMIC database have shown that somatic mutations in the RNF43 locus were also identified in tumor samples from the skin, ovary, breast, prostate, liver, pancreas, and large intestine, suggesting that RNF43 functions as a tumor suppressor in many tissues.…”

Overexpression of collagen VI α3 in gastric cancer

“…The gene interaction network was constructed using a gene expression pattern scanner (GePS: http://www.genomatix.de/) as described previously (10). …”

Nanosecond Pulsed Electric Field Inhibits Cancer Growth Followed by Alteration in Expressions of NF-κB and Wnt/β-Catenin Signaling Molecules

“…At 1 h after nsPEF treatment, the treated survival cells at the same number were obtained to perform transwell assays based on transwell chambers (Millipore, USA), reflecting cell metastasis and invasion ability, as previously described [14]. …”

“…At 1 h after nsPEF treatment, the treated cells were obtained to determine single-cell apoptosis using the assay of TdT–dUTP Terminal Nick-end Labeling (TUNEL) with In Situ Cell Death Detection Kit (Millipore, USA) according to manufacturer’s instruction, as previously described [14]. …”