Building trust and understanding its relationship with online purchasing decisions is important to business-to-consumer (B2C) e-commerce firms seeking to extend their consumers reach globally. Based on the StimulusOrganism-Response (S-O-R) model, this paper examines the moderating role of culture on the relationship between B2C web design (web accessibility, visual appearance and social networking services (SNS)) and interpersonal trust (iTrust), cognitive and affect-based trust that trigger online purchasing intentions. Motivation of this study includes, testing and comparing individual consumer level cultural (individualism and uncertainty avoidance) values as moderators in our research model across two different societies (Australia and Pakistan). The data of the survey were analysed using structural equation modelling-partial least square (SEM-PLS) approach. The results highlight the need to consider cultural differences when identifying the mix of web design strategies to employ in B2C e-commerce websites, not only at the country level but also in one culturally diverse country such as Australia.Keywords: B2C; business-to-consumer; e-commerce; e-business; culture; cognitive-based trust; affect-based trust; purchase intention; iTrust; interpersonal trust; reflective and formative construct; Australia; Pakistan.Reference to this paper should be made as follows: Sohaib, O. and Kang, K. (2015) 'Individual level culture influence on online consumer iTrust aspects towards purchase intention across cultures: A S-O-R model', Int. J. Electronic Business, Vol. 12, No. 2, Biographical notes: Osama Sohaib is perusing PhD (2012PhD ( -2015 in Information Systems and a causal academic at Faculty of Engineering and Information Technology, University of Technology, Sydney. His research interest includes trust, usability and cultural issues in e-commerce, innovation, knowledge management and design thinking.Kyeong Kang, PhD is a Lecturer in Faculty of Engineering and Information Technology, University of Technology, Sydney. Her current research interest includes, impact of the cognition, collaboration and cultural issues in e-services. Her research publications include papers in prestigious journals and conferences.