Review:  infrared spectrometry

McDonald, Robert

doi:10.1021/ac00122a003

Cited by 48 publications

(13 citation statements)

References 294 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…infrared spectroscopy is a powerful tool for studies of the composition and structure of cellular components (1,49,50). Тechnological progress has enhanced the sensitivity of infrared spectroscopy, as it has further facilitated the vibrational spectra analysis, in particular the analysis of the spectral parameters such as frequency, intensity, band shape and band splitting (33,55,56). the main objective of this review is to evaluate Fourier transform infrared spectroscopy (FtiR) as a non-invasive, high effective and cost-advantageous method for identifying biochemical changes as biomarkers for cancer detection.…”

Section: Introductionmentioning

confidence: 99%

Application of Fourier Transform Infrared Spectroscopy for Tumor Diagnosis

Simonova

Karamancheva

2013

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Application of Fourier Transform Infrared Spectroscopy for Tumor Diagnosis

Simonova

Karamancheva

2013

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

“…Even small changes in the distance between atoms or the masses from isotopic substitution alter the rotational and vibrational transition energies of the molecule. The power of vibrational and rotational spectroscopies has been demonstrated in the frequency [3][4][5] and time [6,7] domains in the infrared and microwave. The sensitivity of vibrational spectroscopy has reached the single bond level by the detection of the vibrationally inelastic electron tunneling process using the scanning tunneling microscope (STM) [8].…”

mentioning

confidence: 99%

Rotational and Vibrational Excitations of a Hydrogen Molecule Trapped within a Nanocavity of Tunable Dimension

Toledo

et al. 2013

Phys. Rev. Lett.

View full text Add to dashboard Cite

The rotational and vibrational transitions of a hydrogen molecule weakly adsorbed on the Au(110) surface at 10 K were detected by inelastic electron tunneling spectroscopy with a scanning tunneling microscope. The energies of the j=0 to j=2 rotational transition for para-H(2) and HD indicate that the molecule behaves as a three-dimensional rigid rotor trapped within the tunnel junction. An increase in the bond length of H(2) was precisely measured from the downshift in the rotational energy as the tip-substrate distance decreases.

show abstract

“…Currently, methodological and technological advances are greatly enhancing the sensitivity of infrared spectroscopy. In particular, appreciation of the role of pressure on spectral parameters such as frequency, intensity, band shape, and band splitting and the consequent advances in high-pressure instrumentation have further facilitated the analysis of vibrational spectra (8)(9)(10).…”

mentioning

confidence: 99%

Human colorectal cancers display abnormal Fourier-transform infrared spectra.

Rigas

Morgello

Goldman

et al. 1990

Proc. Natl. Acad. Sci. U.S.A.

218

142

View full text Add to dashboard Cite

Fourier-transform infrared spectroscopy (FT-IR) was applied to the study of tissue sections of human colorectal cancer. Pairs of tissue samples from colorectal cancer and histologically normal mucosa 5-10 cm away from the tumor were obtained from 11 patients who underwent partial colectomy. All cancer specimens displayed abnormal spectra compared with the corresponding normal tissues. These changes involved the phosphate and C-O stretching bands, the CH stretch region, and the pressure dependence of the CH2 bending and C=-O stretching modes. Our findings indicate that in colonic malignant tissue, there are changes in the degree of hydrogen-bonding of (i) oxygen atoms of the backbone of nucleic acids (increased); (ii) OH groups of serine, tyrosine, and threonine residues (any or all of them) of cell proteins (decreased); and (iii) the C=O groups of the acyl chains of membrane lipids (increased). In addition, they indicate changes in the structure of proteins and membrane lipids (as judged by the changes in their ratio of methyl to methylene groups) and in the packing and the conformational structure of the methylene chains of membrane lipids. The cell(s) of the malignant colon tissues responsible for these spectral abnormalities is unknown. Cultured colon adenocarcinoma cell lines displayed similarly abnormal FT-IR spectra. The diagnostic potential of the observed changes is discussed.In recent years spectral methods have been used in the evaluation of malignancy, and some attempts have been made to utilize them as a diagnostic tool. Most of the work involves NMR spectroscopy (1-3); however, the initial enthusiasm for the diagnostic usefulness of NMR has subsided considerably (4).Infrared spectroscopy is becoming an increasingly powerful tool for the study of the composition and structure of cellular components within intact tissues (5-7). Currently, methodological and technological advances are greatly enhancing the sensitivity of infrared spectroscopy. In particular, appreciation of the role of pressure on spectral parameters such as frequency, intensity, band shape, and band splitting and the consequent advances in high-pressure instrumentation have further facilitated the analysis of vibrational spectra (8-10).In this paper we report our findings from the analysis of human colon cancer tissues by Fourier transform infrared (FT-IR) spectroscopy that was also combined with the use of high-pressure (pressure-tuning) infrared spectroscopy, when appropriate. Our data, showing clear-cut spectral differences between normal and malignant colonic tissue, suggest the potential applicability of this approach to a host of biological problems. PATIENTS AND METHODSPatients. The 11 patients whose tissue samples were studied underwent partial large-bowel resection for colorectal cancer at North Shore University Hospital-Cornell University Medical College, Manhasset, NY. Five were women and 6 were men; their average age was 75 years (range, 59-84). Staging of tumors using the modified Dukes' classification (11) showed...

show abstract

Review: infrared spectrometry

Cited by 48 publications

References 294 publications

Application of Fourier Transform Infrared Spectroscopy for Tumor Diagnosis

Application of Fourier Transform Infrared Spectroscopy for Tumor Diagnosis

Rotational and Vibrational Excitations of a Hydrogen Molecule Trapped within a Nanocavity of Tunable Dimension

Human colorectal cancers display abnormal Fourier-transform infrared spectra.

Contact Info

Product

Resources

About