Review of major sweetpotato pests in Japan, with information on resistance breeding programs

Okada, Yoshihiro; Kobayashi, Akira; Tabuchi, Hiroaki; Kuranouchi, Toshikazu

doi:10.1270/jsbbs.16145

Cited by 23 publications

(18 citation statements)

References 55 publications

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“…However, there are few sweet potato cultivars resistant to sweet potato virus disease in the field, especially in East Africa where there is a great demand for virus‐resistant sweet potatoes for subsistence farming. Additionally, resistance observed under experimental conditions may not be reproducible in the field (Okada et al , 2017). It has been documented that CSR3 of SPCSV targets the host plant's RNAi system to cripple the antiviral defence system and increase sweet potato sensitivity to other RNA viruses.…”

Section: Discussionmentioning

confidence: 99%

Development of FRET‐based high‐throughput screening for viral RNase III inhibitors

Wang

Saarela

Poque

et al. 2020

Molecular Plant Pathology

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The class 1 ribonuclease III (RNase III) encoded by Sweet potato chlorotic stunt virus (CSR3) suppresses RNA silencing in plant cells and thereby counters the host antiviral response by cleaving host small interfering RNAs, which are indispensable components of the plant RNA interference (RNAi) pathway. The synergy between sweet potato chlorotic stunt virus and sweet potato feathery mottle virus can reduce crop yields by 90%. Inhibitors of CSR3 might prove efficacious to counter this viral threat, yet no screen has been carried out to identify such inhibitors. Here, we report a novel high-throughput screening (HTS) assay based on fluorescence resonance energy transfer (FRET) for identifying inhibitors of CSR3. For monitoring CSR3 activity via HTS, we used a small interfering RNA substrate that was labelled with a FRETcompatible dye. The optimized HTS assay yielded 109 potential inhibitors of CSR3 out of 6,620 compounds tested from different small-molecule libraries. The three best inhibitor candidates were validated with a dose-response assay. In addition, a parallel screen of the selected candidates was carried out for a similar class 1 RNase III enzyme from Escherichia coli (EcR3), and this screen yielded a different set of inhibitors. Thus, our results show that the CSR3 and EcR3 enzymes were inhibited by distinct types of molecules, indicating that this HTS assay could be widely applied in drug discovery of class 1 RNase III enzymes. K E Y W O R D Sfluorescence resonance energy transfer, high-throughput screening, RNA silencing suppressor, RNase III, small interfering RNA

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Section: Discussionmentioning

confidence: 99%

Development of FRET‐based high‐throughput screening for viral RNase III inhibitors

Wang

Saarela

Poque

et al. 2020

Molecular Plant Pathology

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“…These species are almost morphologically indistinguishable and phylogenetically very close (De Beer et al 2014; Kroon et al 2012; Martin et al 2014), but they were reported on very different hosts: P. lateralis attacks Chamaecyparis spp. and other Cupressaceae (Hansen et al 2000; Robin et al 2011), and C. fimbriata is the agent of sweet potato black rot (Okada et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Real-time loop-mediated isothermal amplification: an early-warning tool for quarantine plant pathogen detection

et al. 2019

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An effective framework for early warning and rapid response is a crucial element to prevent or mitigate the impact of biological invasions of plant pathogens, especially at ports of entry. Molecular detection of pathogens by using PCR-based methods usually requires a well-equipped laboratory. Rapid detection tools that can be applied as point-of-care diagnostics are highly desirable, especially to intercept quarantine plant pathogens such as Xylella fastidiosa , Ceratocystis platani and Phytophthora ramorum , three of the most devastating pathogens of trees and ornamental plants in Europe and North America. To this aim, in this study we developed three different loop mediated isothermal amplification (LAMP) assays able to detect each target pathogen both in DNA extracted from axenic culture and in infected plant tissues. By using the portable instrument Genie ® II, the LAMP assay was able to recognize X. fastidiosa , C. platani and P. ramorum DNA within 30 min of isothermal amplification reaction, with high levels of specificity and sensitivity (up to 0.02 pg µL −1 of DNA). These new LAMP-based tools, allowing an on-site rapid detection of pathogens, are especially suited for being used at ports of entry, but they can be also profitably used to monitor and prevent the possible spread of invasive pathogens in natural ecosystems.

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“…This may be one of the reasons why the virus is widely distributed in most regions of the country. In addition, movement of the infected storage tubers could also introduce viruses within the small holder farms, as storage roots have been reported to spread the viruses through vegetative propagation (Okada et al, 2017). Coat protein genes have been used in taxonomy of begomoviruses (Padidam et al, 1999) with the nucleotide and amino acid cutoff score of <89 and <90% respectively.…”

Section: Discussionmentioning

confidence: 99%

Occurrence and genetic variability of partial coat protein gene of Sweet potato leaf curl virus (SPLCV) in Kenya

Maina¹,

Miano²,

Mbogo³

et al. 2017

Afr. J. Biotechnol.

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A survey was conducted to determine the occurrence of sweet potato leaf curl virus (SPLCV) infecting sweet potato in Kenya. Vine cuttings of sweet potato were collected in 2011 from fields in Central, Western and Coastal regions of the country. The cuttings were grown in an insect-proof screen-house and scion grafted to Ipomoea setosa indicator plants. DNA was extracted from leaf samples of graftinoculated I. serosa and tested for the presence of SPLCV by polymerase chain reaction (PCR) using degenerate and specific primers. The virus was detected in all the regions with incidence rates of 33, 17.0 and 2.6% from Western, Coastal and Central regions, respectively using specific primers. Coat protein gene AV1 from isolates collected from each region was sequenced and their comparison revealed >88.4% nucleotide identity. Phylogenetic grouping using nucleotide and amino acid sequences showed that four Kenyan isolates (Coast3, Central13, Coast15 and Western14) clustered together, while the other remaining five grouped a long with isolates from different parts of the world. The results reveal that Kenyan SPLCV isolates have a clear diversity and are also closely related with other isolates from different parts of the world. The wide distribution of the virus within the country means that urgent measures are needed to clean the farmers planting material to reduce possible losses incurred due to the presence of the virus.

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Review of major sweetpotato pests in Japan, with information on resistance breeding programs

Cited by 23 publications

References 55 publications

Development of FRET‐based high‐throughput screening for viral RNase III inhibitors

Development of FRET‐based high‐throughput screening for viral RNase III inhibitors

Real-time loop-mediated isothermal amplification: an early-warning tool for quarantine plant pathogen detection

Occurrence and genetic variability of partial coat protein gene of Sweet potato leaf curl virus (SPLCV) in Kenya

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