Review of Methods for the Measurement of Oxygen Transfer in Microbial Systems

“…A good review about the published methods for different chemical and microbial model systems is given by Sobotka et al (1982). The described methods are often divided into two groups according to their applicability either to chemical model systems (Bartholomew et al, 1950;Cooper et al, 1944;Lara Marquez et al, 1994;Linek, 1966;Morimoto et al, 1979) or to microbial systems (Anderlei and Büchs, 2001;Bandyopadhyay et al, 1967;Duetz et al, 2000;Henzler and Schedel, 1991;Hirose et al, 1966;Hospodka, 1966;McDaniel and Bailey, 1969).…”

Section: Introductionmentioning

confidence: 98%

Optical method for the determination of the oxygen‐transfer capacity of small bioreactors based on sulfite oxidation

Hermann

Walther

Maier

et al. 2001

Biotech & Bioengineering

103

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The growth of microorganisms may be limited by operating conditions which provide an inadequate supply of oxygen. To determine the oxygen-transfer capacities of small-scale bioreactors such as shaking flasks, test tubes, and microtiter plates, a noninvasive easy-to-use optical method based on sulfite oxidation has been developed. The model system of sodium sulfite was first optimized in shaking-flask experiments for this special application. The reaction conditions (pH, buffer, and catalyst concentration) were adjusted to obtain a constant oxygen transfer rate for the whole period of the sulfite oxidation reaction. The sharp decrease of the pH at the end of the oxidation, which is typical for this reaction, is visualized by adding a pH dye and used to measure the length of the reaction period. The oxygen-transfer capacity can then be calculated by the oxygen consumed during the complete stoichiometric transformation of sodium sulfite and the visually determined reaction time. The suitability of this optical measuring method for the determination of oxygen-transfer capacities in small-scale bioreactors was confirmed with an independent physical method applying an oxygen electrode. The correlation factor for the maximum oxygen-transfer capacity between the chemical model system and a culture of Pseudomonas putida CA-3 was determined in shaking flasks. The newly developed optical measuring method was finally used for the determination of oxygen-transfer capacities of different types of transparent small-scale bioreactors.

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“…Judat [10] critically reviewed gasliquid mass transfer in agitated vessels. On the other hand, Sobotka et al [11] reviewed the measuring methods for oxygen transfer in microbial systems. Nienow and Ulbrecht [12] reported on mixing and mass transfer in high viscosity liquids.…”

Section: Introductionmentioning

confidence: 99%

Mass Transfer Coefficients in Mechanically Agitated Gas‐Liquid Contactors

2007

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There are a large number of correlations given in literature for the prediction of volume-related liquid-side mass transfer coefficients in mechanically agitated gasliquid contactors. Significant disagreement can be observed concerning the proposed correlations, so that no single correlation exists representing all of the mass transfer data given in the literature. The observed differences can mainly be ascribed to the differences in the geometry of the system, the range of operational conditions and the measurement method used. On the basis of a comparative study of mass transfer phenomena in agitated Newtonian and non-Newtonian aerated liquids, a critical discussion of the literature results is presented in this review article, so that final conclusions can be drawn for the k L a values in the different single-and multiple-impeller agitated systems studied in the literature.

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Review of Methods for the Measurement of Oxygen Transfer in Microbial Systems

Cited by 62 publications

References 83 publications

Effect of elevated oxygen and glutamine levels on foreign protein production at high cell densities using the insect cell-baculovirus expression system

Effect of elevated oxygen and glutamine levels on foreign protein production at high cell densities using the insect cell-baculovirus expression system

Optical method for the determination of the oxygen‐transfer capacity of small bioreactors based on sulfite oxidation

Mass Transfer Coefficients in Mechanically Agitated Gas‐Liquid Contactors

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