Review of positive direct antiglobulin tests found on cord blood sampling

Dinesh, Dorothy

doi:10.1111/j.1440-1754.2005.00692.x

Cited by 70 publications

(67 citation statements)

References 21 publications

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“…Dinesh et al observed that ABO incompatibility was the leading cause of HDFN in countries with high human development index and similar results were obtained in this study. 21 All mothers of infants with ABO-HDFN were invariably Group O. This observation made it clear that in Group O serum, IgG was the major isotype for anti-A and anti-B.…”

Section: Discussionmentioning

confidence: 79%

Frequency of Antibodies in Haemolytic Disease of Foetus and Newborn

Vilambil¹,

Dharmadas²,

Usha³

et al. 2017

jemds

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BACKGROUNDHaemolytic disease of foetus and newborn (HDFN) is triggered by enhanced destruction of red blood cell due to immune-mediated destruction, intrinsic abnormalities, acquired aberrations, etc. Lifespan of infant's erythrocytes are shortened by the action of placentally transferred maternal antibodies in immune-mediated HDFN.The aim of this study was to identify the frequency of maternal antibodies in neonates with hyperbilirubinaemia. Settings and Design-This was a cross-sectional study in neonates admitted to neonatal intensive care unit (NICU). Setting for the research was Dept. of Transfusion Medicine and Neonatology division of Sree Avittom Thirunal Hospital for women and children attached to Govt. Medical College, Trivandrum.

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Section: Discussionmentioning

confidence: 79%

Frequency of Antibodies in Haemolytic Disease of Foetus and Newborn

Vilambil¹,

Dharmadas²,

Usha³

et al. 2017

jemds

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show abstract

“…According to Levine et al and Schonitzer et al DAT was neither a tool for diagnosis nor a predictor of severity of disease, while it was found to be beneficial for establishing ABO HDFN by Bel et al, Dufour et al and Whyte et al 24,25,5,26,27 Dinesh had postulated that in infants with clinically significant haemolysis, 80% had a positive DAT. 28 While Bjarte and Morten opined that antepartum and postpartum serologic tests were poor predictors of ABO haemolytic disease, Umit et al suggested that reticulocyte count, a positive DAT and the presence of a sibling with neonatal jaundice were good predictors for the development of significant hyperbilirubinaemia and severe haemolytic disease. 2,29 Dufour concluded that positive DAT and strength of reaction in heat eluate could be used as predictors of prognosis.…”

Section: Discussionmentioning

confidence: 99%

“…2 Dinesh et al had observed that in infants with clinically significant haemolysis, 80% had a positive DAT. 28 Kaplan et al reported that DAT positive ABO heterospecificity was associated with increased haemolysis and a higher incidence of hyperbilirubinaemia. 30 According to Quinn et al, coombs positivity and strong elution positivity might be a helpful predictor of jaundice but not of its severity.…”

Section: Discussionmentioning

confidence: 99%

Immunohaematological Profile of Maternal Antibody-Mediated Abo Haemolytic Disease of Foetus and Newborn

Vilambil¹,

Dharmadas²,

Usha³

et al. 2017

jemds

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BACKGROUNDThe potency of anti-A and anti-B was determined by racial and environmental factors. With respect to ethnic groups, the prevalence and intensity of maternal-newborn ABO incompatibility varied in different populations. The aim of this study is to describe the immunological profile of ABO HDFN. Settings and Design-This was a descriptive study conducted in neonates with ABO HDFN. Research was conducted in Depts. of Transfusion Medicine and Neonatology division of Paediatrics in Govt. Medical College, Trivandrum. MATERIALS AND METHODSThose neonates who fulfilled the inclusion criteria were enrolled in this study. Demographic details, maternal details, bilirubin and haemoglobin levels were recorded. Investigations such as maternal blood grouping and antibody titration were done. In infant's blood sample direct antiglobulin test, blood grouping and elution was performed.Statistical Analysis-All statistical data were analysed using SPSS software version 16. RESULTSAmong mothers of infants with ABO HDFN 25 (22.7%) had a titre of 128, 34 (30.9%) had 256, 35 (31.8%) had 512 and 16 (14.6%) had 1024. Among 110 infants 34 (30.9%) were DAT positive, while the rest 76 (69.1%) were DAT negative. While DAT positive was graded 13 (11.8%) fell in grade 1, 16 (14.6%) in grade 2, 3 (2.7%) in grade 3 and 2 (1.8%) in grade 4 categories. Eluate from 59 (53.6%) of infants with ABO HDFN gave a positive result, while the rest 51 (46.4%) gave a negative result. Out of that 59 (53.6%) elution positive infants, 34 (30.9%) had a positive DAT and 25 (22.7%) had a negative DAT; 51 (46.4%) of infants were negative for both elution and DAT. CONCLUSIONMothers of infants with ABO HDFN had an antibody titre of 128 -1024. Almost one-third of infants were DAT positive and majority fell in grade 1 or 2. Eluate from half of infants gave a positive result.

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“…10 Skimunarprófið Direct antiglobulin test (DAT, einnig kallað Coombs-próf) greinir mótefni bundin við rauð blóðkorn. 11 Notkun þess var fyrst lýst af Coombs og félögum árið 1946. 12 Á Íslandi er DAT gert á nafla- strengsblóði frá öllum nýburum RhD-neikvaeðra maeðra og einnig nýburum kvenna sem greinst hafa með klínískt mikilvaeg rauð-kornamótefni á meðgöngu.…”

Section: áGripunclassified