Review of the cultivation program within the National Alliance for Advanced Biofuels and Bioproducts

Lammers, Peter J.; Huesemann, Michael H.; Boeing, Wiebke J.; Anderson, David M.; Arnold, Robert G.; Bai, Xuemei; Bhole, Manish R.; Brhanavan, Yalini; Brown, Leanne; Brown, Jola; Brown, Judith K.; Chisholm, Stephen T.; Downes, C. Meghan; Fulbright, Scott; Ge, Yufeng; Holladay, Johnathan E.; Ketheesan, Balachandran; Khopkar, Avinash R.; Koushik, Ambica; Laur, Paul; Marrone, Babetta L.; Mott, Joe L.; Nirmalakhandan, Nagamany; Ogden, Kimberly L.; Parsons, Ronald L.; Polle, Juergen; Ryan, Randy; Samocha, Tzachi M.; Sayre, Richard T.; Seger, Mark; Selvaratnam, Thinesh; Sui, Ruixiu; Thomasson, J. Alex; Unc, Adrian; Voorhies, Wayne Van; Waller, Peter; Yao, Yao; Olivares, José A.

doi:10.1016/j.algal.2016.11.021

Cited by 77 publications

(47 citation statements)

References 52 publications

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“…It performed well outdoors, producing up to 30 g/m 2 /d biomass with a lipid content of 25%. Cultivation results are discussed in detail by Lammers and coworkers [14]. This positive result contrasted the previously published Aquatic Species Program report from 1998 [7], which indicated that it would be impossible to match a successful combination of indoor isolation/screening/testing with outdoor strain performance.…”

Section: Mining Algae's Natural Diversitymentioning

confidence: 60%

“…Importantly, the team structure of NAABB allowed the development of an efficient conduit for transferring lead algal strains or genetic variants to the algal cultivation testbeds for analysis in contained or open pond environments. Former members of the NAABB cultivation team have reported a significant biomass accumulation rate in ponds (30 g/m 2 /d) for C. sorokiniana (DOE1412), which was discovered by the NAABB algal biology team [14]. Furthermore, it has been determined that the energy content of C. sorokiniana biomass can increase by 50% with no loss in biomass yield during cultivation under nitrogen-free conditions [88].…”

Section: Conclusion and Recommendationsmentioning

confidence: 98%

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Review of the algal biology program within the National Alliance for Advanced Biofuels and Bioproducts

et al. 2017

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In 2010, when the National Alliance for Advanced Biofuels and Bioproducts (NAABB) consortium began, little was known about the molecular basis of algal biomass or oil production. Very few algal genome sequences were available and efforts to identify the best-producing wild species through bioprospecting approaches had largely stalled after the U.S. Department of Energy's Aquatic Species Program. This lack of knowledge included how reduced carbon was partitioned into storage products like triglycerides or starch and the role played by metabolite remodeling in the accumulation of energy-dense storage products. Furthermore, genetic transformation and metabolic engineering approaches to improve algal biomass and oil yields were in their infancy. Genome sequencing and transcriptional profiling were becoming less expensive, however; and the tools to annotate gene expression profiles under various growth and engineered conditions were just starting to be developed for algae. It was in this context that an integrated algal biology program was introduced in the NAABB to address the greatest constraints limiting algal biomass yield. This review describes the NAABB algal biology program, including hypotheses, research objectives, and strategies to move algal biology research into the twenty-first century and to realize the greatest potential of algae biomass systems to produce biofuels.

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Section: Mining Algae's Natural Diversitymentioning

confidence: 60%

Section: Conclusion and Recommendationsmentioning

confidence: 98%

Review of the algal biology program within the National Alliance for Advanced Biofuels and Bioproducts

et al. 2017

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“…The Chlorella sorokiniana strain DOE 1412, also referred to as NAABB 2412 (Lammers et al 2017 (Rippka and Herdman 1993). Cultures were maintained by periodic serial transfer on solid BG-11 medium containing 30 g L −1 of agar.…”

Section: Algal Culturementioning

confidence: 99%

“…The outdoor cultivation of microalgae presents a number of challenges, including susceptibility to attack by bacterial and viral pathogens and predators. The field isolate of Chlorella sorokiniana (Shihira and Krauss 1965) [Chlorophyta], designated DOE 1412 (Lammers et al 2017) is considered one of the most promising biofuel production strains, based on a potential maximum growth rate of 5.9 day −1 at 36°C, as determined under a range of simulated temperatures (13-45°C) analogous to those experienced in arid-land microalgal cultivation locales such as the southwestern USA and other arid climates (Huesemann et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Real-time quantitative detection of Vampirovibrio chlorellavorus, an obligate bacterial pathogen of Chlorella sorokiniana

Steichen

Brown

2018

J Appl Phycol

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Vampirovibrio chlorellavorus is an obligate, predatory bacterial pathogen of the genus Chlorella. It is recognized as an important pathogen of Chlorella sorokiniana, field isolate DOE 1412, a highly-favored microalga for cultivation in outdoor reactors in the arid USA Southwest for feedstocks used in biofuel production. To determine the V. chlorellavorus titer, based on gene copy number, required to cause infection and mortality of C. sorokiniana in an experimental outdoor reactor, a multiplexed quantitative polymerase chain reaction (qPCR) assay was developed for pathogen detection, based on the 16S and 18S ribosomal RNA gene of V. chlorellavorus and C. sorokiniana, respectively. The assay was further used to establish the optimal effective concentration of benzalkonium chloride required to achieve a below Bdisease-threshold^-bacterial titer, while minimizing biocidal effects on algal growth and enable economic biomass production. Reactors treated with 2.0 ppm benzalkonium chloride at four-day intervals throughout the cultivation cycle experienced runs of 22 days or longer, compared to 12 days for the untreated control. The qPCR assay was used to estimate disease severity over time using the Area Under the Disease Progress Stairs (AUDPS) metric, indicating a severity rating of 0.016 and 62.308 in biocide-treated and untreated cultures, respectively. The near-real time assay detected as few as 13 copies of V. chlorellavorus, allowing for the recognition of its presence in the reactor just before algal cell density decreased, an indication of pathogen attack, while also informing the timing of biocide applications to minimize DOE 1412 infection such that harvestable biomass could be produced.

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“…The major compounds of microalgae used for biofuel production are carbohydrates in the form of reducing sugars, proteins as various amino acids, and lipids in the form of fatty acids ( Table 1). Most of previous reviews focused on conversion of a specific component of the biomass (carbohydrates, proteins, or lipids) into biofuel while considering the other portions of the biomass to be a waste, which might be the main cause for the economic infeasibility of microalgal biofuels [17][18][19][20]. In this review, we explored the use of all three major biocomponents of microalgal biomass including carbohydrates, proteins, and lipids for maximum biofuel generation.…”

Section: Introductionmentioning

confidence: 99%

Utilization of Microalgal Biofractions for Bioethanol, Higher Alcohols, and Biodiesel Production: A Review

et al. 2017

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Abstract:Biomass is a crucial energy resource used for the generation of electricity and transportation fuels. Microalgae exhibit a high content of biocomponents which makes them a potential feedstock for the generation of ecofriendly biofuels. Biofuels derived from microalgae are suitable carbon-neutral replacements for petroleum. Fermentation is the major process for metabolic conversion of microalgal biocompounds into biofuels such as bioethanol and higher alcohols. In this review, we explored the use of all three major biocomponents of microalgal biomass including carbohydrates, proteins, and lipids for maximum biofuel generation. Application of several pretreatment methods for enhancement the bioavailability of substrates (simple sugar, amino acid, and fatty acid) was discussed. This review goes one step further to discuss how to direct these biocomponents for the generation of various biofuels (bioethanol, higher alcohol, and biodiesel) through fermentation and transesterification processes. Such an approach would result in the maximum utilization of biomasses for economically feasible biofuel production.

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Review of the cultivation program within the National Alliance for Advanced Biofuels and Bioproducts

Cited by 77 publications

References 52 publications

Review of the algal biology program within the National Alliance for Advanced Biofuels and Bioproducts

Review of the algal biology program within the National Alliance for Advanced Biofuels and Bioproducts

Real-time quantitative detection of Vampirovibrio chlorellavorus, an obligate bacterial pathogen of Chlorella sorokiniana

Utilization of Microalgal Biofractions for Bioethanol, Higher Alcohols, and Biodiesel Production: A Review

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