Atmospheric pressure plasma jets are gaining a lot of
attention
due to their widespread applications in the field of bio-decontamination,
polymer modification, material processing, deposition of thin film,
and nanoparticle fabrication. Herein, we are reporting the disinfection
of Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia
coli bacteria using plasma jet. In this regard, Ar–O2, Ar–N2, and Ar–O2–N2 mixture plasma is generated and characterized using optical
and electrical characterization. Variation in plasma parameters like
electron temperature, electron density, and reactive species production
is monitored with discharge parameters such as applied voltage and
feed gas concentration. Results show that the peak average power consumed
in Ar–O2, Ar–N2, and Ar–O2–N2 mixture plasma is found to be 4.45,
2.93, and 4.35 W respectively, at 8 kV. Moreover, it is noted that
by increasing applied voltage, the electron temperature, electron
density, and reactive species production also increases. It is worth
noting that electron temperature increases with increase in oxygen
concentration in the mixture (
, while it decreases with increase in nitrogen
concentration in the mixture (Ar–N2). Similarly,
a decreasing trend in electron temperature is noted for Ar–O2–N2 mixture plasma. On the other hand, a
decreasing trend in electron density is noted for all the mixtures.
Reduction in viable colonies of Pseudomonas
aeruginosa, Staphylococcus
Aureus, and Escherichia
coli were confirmed by the serial
dilution method. The inactivation efficiency of pulsed DC plasma generated,
in the Ar–N2 mixture at 8 kV and 6 KHz, was evaluated
against P. aeruginosa, S. aureus and E. coli bacteria by measuring the number of surviving cells versus plasma
treatment time. Results showed that after 240 s of plasma treatment,
the number of survival colonies of the mentioned bacteria was reduced
to less than 30 CFU/mL.