Review: Recent Developments in Enzyme-Based Biosensors for Biomedical Analysis

Ispas, Cristina; Crivat, Georgeta; Andreescu, Silvana

doi:10.1080/00032719.2011.633188

Cited by 161 publications

(85 citation statements)

References 107 publications

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“…The incorporation of bio-materials [161,162] into electrochemical sensors enables a level of selectivity that is comparable to that seen in nature, with the majority of advancements i n b i o s e n s o r s d u e t o t h e i m m o b i l i z a t i o n o f a b i o l o g i c a l m a t e r i a l ( a n d h e n c e i t ' s physiochemical properties to an electrode's surface electrode [163,164]. Mimicking nature selectivity has been attempted with the use of molecularly imprinted polymers but so far has not rivalled the selectivity of biomolecules such as antibodies, nucleic acids, aptamers, etc.…”

Section: Voltammetric Sensorsmentioning

confidence: 99%

Electroanalytical Sensor Technology

Power¹,

Morrin²

2013

Electrochemistry

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Section: Voltammetric Sensorsmentioning

confidence: 99%

Electroanalytical Sensor Technology

Power¹,

Morrin²

2013

Electrochemistry

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“…Electrochemical biosensors are attracting major interest because of their potential for a myriad theranostics and environmental applications [1][2][3]. Moreover, they are good candidates for the basis of portable point-of-care devices, due to their inherent sensitivity, specificity, usability, miniaturisation and cost-effectiveness [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Control of Electron‐transfer in Immunonanosensors by Using Polyclonal and Monoclonal Antibodies

et al. 2016

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The design and operation of biosensors is not trivial. For instance, variation in the output signal during monitoring of analytes can not usually be controlled. Hence, if such control were possible, and could be triggered on demand, it would greatly facilitate system design and operation. Herein, we report the design of two types of voltamperometric immunosensors, in which the magnitude of the current output signal (differential pulse voltammetry [DPV]) can be increased or decreased as needed. The designed systems use monoclonal and polyclonal anti‐human IgG antibodies, conjugated to monopodal ferrocene‐modified gold nanoparticles that are casted onto screen‐printed carbon electrodes (Ab/mFcL/AuNPs/SPCEs). Upon addition of human IgG as antigen, the systems exhibit opposite responses according to the Ab: the current decreases when monoclonal Ab is used, whereas it increases when polyclonal Ab is used. We attributed the former response to inhibition of electron‐transfer (due to the formation of a protein layer), and the latter response, to a global increase in electron transfer (induced by the aggregation of gold nanoparticles). These effects were confirmed by studying a custom‐made lipoic acid‐based bipodal ligand, which confirmed that the increase in current is effectively induced by the aggregation of the modified nanoparticles (pAb/mFcL/AuNPs). Both sensors have large dynamic ranges, although the pAb‐based one was found to be 3.3‐times more sensitive. Tests of selectivity and specificity for ovalbumin, α‐lactalbumin and serum bovine albumin showed that the immunosensors are highly selective and specific, even in the presence of up to 1000‐fold levels of potentially competitive proteins. The limit of detection for human IgG using the pAb/mFcL/AuNP bioconjugate was estimated to be 0.85 ng/mL. The pAb/mFcL/AuNPs‐based biosensor has used to determine amounts of human IgG in real sample.

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“…[5] Among the investigated polymers, polyaniline (PANI), polypyrrole, poly(o-phenylenediamine) (Po-PDA) and poly(aminophenol) (PAP) have recently been paid a lot of attention for their application in biochemistry, biosensor and the immobilization of protein and enzyme. [6] The properties of PANI and PANI derivatives have been improved by molecular design, modification of monomer structure, and preparation of composites and co-polymers. [7,8] Copolymerization is one of the simplest methods for improving the properties of PANI and its derivatives.…”

Section: Introductionmentioning

confidence: 99%

Non-conventional synthesis and antibacterial activity of poly(aniline-co-o-phenylenediamine)/bentonite nanocomposites

Al-Hussaini

Eldars

2013

Designed Monomers and Polymers

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Copolymers of aniline and o-phenylenediamine/hydrophilic bentonite nanocomposites were synthesized by 5:1 M ratios of the respective monomers with different percentages of nanoclay via modified in situ chemical copolymerization. The results were justified by measuring the FT-IR for PANI-o-PDA/bentonite nanocomposites. The thermal stability was studied. PANI-o-PDA/bentonite nanocomposites were thermally more stable than pure polymer. The antibacterial activities of PANI-o-PDA/bentonite nanocomposites towards both Gram-positive and Gram-negative bacteria were investigated.

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Review: Recent Developments in Enzyme-Based Biosensors for Biomedical Analysis

Cited by 161 publications

References 107 publications

Electroanalytical Sensor Technology

Electroanalytical Sensor Technology

Control of Electron‐transfer in Immunonanosensors by Using Polyclonal and Monoclonal Antibodies

Non-conventional synthesis and antibacterial activity of poly(aniline-co-o-phenylenediamine)/bentonite nanocomposites

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