Review: Regulation of the cancer epigenome by long non-coding RNAs

Forrest, Megan E.; Khalil, Ahmad M.

doi:10.1016/j.canlet.2017.03.040

Cited by 93 publications

(56 citation statements)

References 123 publications

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“…LncRNAs display complicated regulatory function by a wide range of regulatory mechanism through transcriptional regulation, epigenetic modulation through chromatin modification and post-transcriptional regulation [25][26][27][28][29][30]. However, the involvement of lncRNAs in human GC development and progression are still need to be illustrated.…”

Section: Discussionmentioning

confidence: 99%

Long Noncoding RNA EGFR-AS1 Promotes Cell Proliferation by Increasing EGFR mRNA Stability in Gastric Cancer

Qian

Peng

et al. 2018

Cell Physiol Biochem

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Background/Aims: LncRNA EGFR-AS1 is an antisense transcript of EGFR, which plays a key role in gastric cancer progression. This study was aimed to explore the effects of lncRNA EGFR-AS1 on GC and the underling mechanisms. Methods: The silencing of EGFR-AS1 expression was performed by using EGFR-AS1 shRNA lentivirus in MGC803 and SGC-7901 GC cell. The levels of lncRNA EGFR-AS1 and EGFR were detected by qPCR and western blot. Cell proliferation was assessed by CCK-8, EdU, and colony formation assays. The EGFR mRNA stability was explored by using RNA synthesis inhibitor α-amanitin. Results: In our study, EGFR-AS1 significantly up-regulated in GC tissues and correlated with tumor size. And the expression of EGFR-AS1 positively correlated with EGFR in tissues. Moreover, knock-down of EGFR-AS1 inhibited the proliferation of GC cells via suppressing EGFR-dependent PI3K/AKT pathway in vitro and in vivo. Mechanismly, depletion of EGFR-AS1 was found to decrease EGFR expression by reduction of EGFR mRNA stability. Conclusion: Our findings suggested that EGFR-AS1 might have an oncogenic effect on GC and serve as a potential target of GC.

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Section: Discussionmentioning

confidence: 99%

Long Noncoding RNA EGFR-AS1 Promotes Cell Proliferation by Increasing EGFR mRNA Stability in Gastric Cancer

Qian

Peng

et al. 2018

Cell Physiol Biochem

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“…Long noncoding RNAs play an important role in pathogenesis of malignancy. 12 One of these lncRNAs is NEAT. It plays an important role in gene transcription by protein sequestration into paraspeckles 13 and regulates messenger RNA export.…”

Section: Neat1mentioning

confidence: 99%

“…Long noncoding RNAs play an important role in pathogenesis of malignancy . One of these lncRNAs is NEAT.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of serum long noncoding RNA NEAT and MiR‐129‐5p in hepatocellular carcinoma

et al. 2019

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Hepatocellular carcinoma (HCC) is one of the most prevalent form of cancer. Various long non coding RNA (lncRNAs) and micro RNA have been confirmed to have a role in the progression of HCC. Our aim was to investigate for the first time the expression profile of serum level of LNC NEAT (nuclear enrich abundant transcript) and MiR‐129‐5p in HCC patients and their relations with patient's clinical and biochemical investigations rather than previous studies on tissue cell lines. Our study includes 72 subjects divided into 36 as control subjects and 36 patients with HCC. Complete physical and laboratory investigations were done on all subjects. RNAs were extracted from sera of all subjects. RNAs were reversed transcribed into cDNAs using Qiagen, Valenica, CA. Quantitative PCR (qPCR) was performed using Rotor gene Q System (Qiagen). Relative NEAT1 expression level was significantly increased in serum of HCC patients 4.7 (1.31–6.82) (p < .0001). Meanwhile MiR‐129‐5p relative expression level was significantly decreased in serum of HCC patients 0.17 (0.14–20) (p < .0001). Also there was negative significant correlation between the expression level of LNC NEAT and MiR‐129‐5p in HCC group (p < .0001). ROC curve analysis revealed that LNC NEAT; AUC = 0.981, p < .0001, cutoff value (1.02), sensitivity 100%, specificity 88.9%. MiR‐129‐5p; AUC = 0.997, p < .0001, cutoff value (0.43), sensitivity 100%, specificity 97.2%. Serum LNC NEAT and MiR‐129‐5p could be used as potential biomarkers for HCC cancer diagnosis and prognosis.

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“…10 Growing studies have concluded that the deregulated expression of lncRNAs are implicated in the tumor formation and progression by acting as oncogenes or tumor promoters. [11][12][13] In addition, the possibility of lncRNAs as new cancer biomarkers attracted growing attention due to the frequent dysregulation and the development of high-throughput sequencing. 14,15 High-mobility group AT-hook 1 (HMGA1), located in 6p21.31, a novel transcriptional regulator of the FoxO1 gene which acts as an important regulator in the cell cycle.…”

Section: Introductionmentioning

confidence: 99%

ELK1‐induced upregulation of lncRNA TRPM2‐AS promotes tumor progression in gastric cancer by regulating miR‐195/ HMGA1 axis

Huang¹,

Chang²,

Wang³

et al. 2019

J of Cellular Biochemistry

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Long noncoding RNAs (lncRNAs) have been confirmed to be aberrantly expressed in various diseases including tumors. Recently, a new tumor‐related lncRNA, lncRNA TRPM2 antisense RNA (TRPM2‐AS), was shown to be involved in many tumors, such as lung cancer and breast cancer. However, the expression and role of TRPM2‐AS in the development of gastric cancer (GC) have not been elucidated. In the current study, we provided evidence that the expression levels of TRPM2‐AS were increased in both GC tissues and cell lines. We also showed that overexpression of TRPM2‐AS was modulated by ELK1, a transcription factor. The results of clinical assays showed that higher expressions of TRPM2‐AS were significantly related with invasion depth, TNM stage, lymphatic metastasis, and shorter overall survival. Further clinical assays using multivariate analysis suggested that TRPM2‐AS expression was an independent prognostic factor in patients with GC. Functional experiments illustrated that depression of TRPM2‐AS suppressed proliferation, migration, and invasion in GC cells. In terms of mechanism, we found that TRPM2‐AS directly inhibited miR‐195, which targeted the 3′‐untranslated region of high‐mobility group AT‐hook 1 (HMGA1) messenger RNA. Overall, these findings revealed that ELK1‐induced overexpression of TRPM2‐AS promoted the development and progression of GC in part through miR‐195/HMGA1 signaling axis, and established its candidacy as a new cancer biomarker for GC patients.

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Review: Regulation of the cancer epigenome by long non-coding RNAs

Cited by 93 publications

References 123 publications

Long Noncoding RNA EGFR-AS1 Promotes Cell Proliferation by Increasing EGFR mRNA Stability in Gastric Cancer

Long Noncoding RNA EGFR-AS1 Promotes Cell Proliferation by Increasing EGFR mRNA Stability in Gastric Cancer

Evaluation of serum long noncoding RNA NEAT and MiR‐129‐5p in hepatocellular carcinoma

ELK1‐induced upregulation of lncRNA TRPM2‐AS promotes tumor progression in gastric cancer by regulating miR‐195/ HMGA1 axis

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