The main objective of this research was to evaluate the use of unmanned aerial vehicles (UAVs) in estimating the aboveground biomass and carbon, and the dasometric characteristics at three different spacings (2.5 m × 1.0 m, 2.5 m × 2.0 m and 2.5 m × 3.0 m) in a silvopastoral system (SPS) for the biomass production of Tectona grandis. A total of 90 trees were sampled, 63 of which were used to perform a dasometric evaluation (vertical and horizontal) in a spacing test in an SPS, and the rest to evaluate the use of UAVs in estimating the aboveground biomass in the spacing test. The results showed significant differences in average diameter at breast height (dbh) between spacings, and in aboveground biomass per tree. The amount of aboveground biomass and carbon per hectare increases at smaller spacings, but the differences were not statistically significant. A logarithmic model was prepared to estimate the dbh based on the crown diameter from the data collected taken in the field, since estimating this variable by means of UAVs is difficult. Significant differences were found in the aboveground biomass estimated using the field data compared to UAV data. The estimation of the crown diameter of the selected trees, hindered by the canopy closure in the SPS, was not adequate, which could influence the amount of aboveground biomass estimated using UAV data.