Revision of the genus Minagrion Santos, 1965 (Odonata: Coenagrionidae)

Abstract

Endemic to Brazil, the genus Minagrion Santos, 1965 is revised for the first time. The genus contains five species, and here we establish diagnostic characters, keys and drawings for both sexes. Furthermore, we propose the synonymy of M. franciscoi Machado & Bedê, 2015 new syn. with M. caldense Santos, 1965.

Odonata (Insecta) richness in Atlantic Forests from Minas Gerais state, Brazil

“…Therefore, this new record for F. siqueirai represents a rediscovery of the species in a protected area, which could be an important step in learning more about its natural history and distribution through new collections in that region and adjacent areas. with M. caldense (Vilela et al 2020). In 2018, this species was assessed as VU (vulnerable) by the IUCN due to its restricted distribution area (<8,000 km²) and continuous decline of its habitats .…”

“…On the other hand, the ICMBio assessment (made in 2022) classified M. caldense as NT (near threatened), which is a criterion close to that established by the IUCN. Problems such as the ongoing habitat decline in the occurrence areas were also mentioned in the ICMBio assessment, and the new records provided by Vilela et al (2020) contributed to modifying the threat level. With the new data presented here, a new assessment of M. caldense could further change again its conservation status, considering that it occurs in another protected area and will enhance the current knowledge of its distribution.…”

Ficha de Minagrion caldense

“…Biomas Cerrado, Mata Atlântica Bacias Hidrográficas Sub-bacia Grande, Sub-bacia São Francisco Alto Distribuição Endêmica do Brasil: Sim Minagrion caldense é endêmica do Brasil, sendo conhecida de quatro localidades em Minas Gerais (Vilela et al, 2020). Os registros em Poços de Caldas são muito antigos e não se sabe da permanência da espécie na região, pois não houve esforço de coleta recente.…”

DNA Barcoding and New Records of Odonates (Insecta: Odonata) from Paraíba State, Brazil

“…The PCR conditions for amplification consisted of 1 × buffer (Colorless GoTaq ® Flexi Buffer; Promega Corp., Madison, WI, USA), 0.2 mM dNTP mix, 0.2 μM of each primer, 2 The PCR conditions for amplification consisted of 1 × buffer (Colorless GoTaq ® Flexi Buffer; Promega Corp., Madison, WI, USA), 0.2 mM dNTP mix, 0.2 µM of each primer, 2 mM MgCl 2 , 1U Taq polymerase (GoTaq ® G2 hot start polymerase, Promega Corp., Madison, WI, USA) and 2 µL of template DNA; these materials were placed in a total reaction volume of 25 µL. The PCR cycling program to OdoF1_t1 and OdoR1_t1 followed Vilela et al [18]. For the BF2-BR2 primers, the PCR cycling program was run as follows: initial denaturation step with 3 min at 95 • C, 35 cycles of denaturation for 30 s at 95 • C, annealing for 45 s at 50 • C and extension for 1 min at 72 • C, and final extension for 5 min at 72 • C. The PCR products were purified with ethanol/sodium acetate and sequenced in an ABI 3130 Genetic Analyzer (Applied Biosystems, Waltham, MA, USA).…”

“…For Neotropical odonates, the use of DNA barcoding is still limited, and there has been some discussion regarding its effectiveness by Koroiva et al [16]. Moreover, Vilela et al [17,18] have substantiated its direct application through integrative taxonomy, i.e., the framework to delimit and describe taxa by integrating information from multiple and complementary perspectives [19], using molecular and morphological data.…”