Revision of the taxonomic status of the species Rhizobium leguminosarum (Frank 1879) Frank 1889AL, Rhizobium phaseoli Dangeard 1926AL and Rhizobium trifolii Dangeard 1926AL. R. trifolii is a later synonym of R. leguminosarum. Reclassification of the strain R. leguminosarum DSM 30132 (=NCIMB 11478) as Rhizobium pisi sp. nov.

Ramírez-Bahena, Martha Helena; García‐Fraile, Paula; Peix, Álvaro; Valverde, Ángel; Rivas, Raúl; Igual, José M.; Mateos, Pedro F.; Martínez‐Molina, Eustoquio; Velázquez, Encarna

doi:10.1099/ijs.0.65621-0

Cited by 155 publications

(79 citation statements)

References 16 publications

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“…31 However, as this reclassification was never validated, all these species remain valid until 2008 in which our investigation group revised the taxonomic status of these species. 60 Our results showed that the valid species are R. leguminosarum and R. phaseoli, whereas R. trifolii is a later synonym of R. leguminosarum. Moreover, a problem with the type strains of R. leguminosarum deposited in different culture collections was detected and a new species named R. pisi was proposed to include one of these two strains deposited in DSMZ and NCIMB collections from Germany and United Kingdom, respectively.…”

Section: Some Problems In Rhizobial Taxonomymentioning

confidence: 84%

“…Although the current classification of bacteria is based in the 16S rRNA gene that has allowed the description of non-nodulating species of rhizobia, it has limitations to differentiate among close species 59,60 and for this purpose several metabolic genes (housekeeping) have been proposed in several groups of bacteria. 61 In rhizobia the two first genes analyzed were recA and atpD 62 and currently they have been sequenced in many rhizobial species showing their usefulness in differentiation of species whose 16S rRNA genes are nearly identical.…”

Section: Recent Changes In the Taxonomy Of Rhizobiamentioning

confidence: 99%

“…61 In rhizobia the two first genes analyzed were recA and atpD 62 and currently they have been sequenced in many rhizobial species showing their usefulness in differentiation of species whose 16S rRNA genes are nearly identical. 59,60 In the last years new schemes for identification and phylogenetic analysis of bacteria named MLSA (Multilocus sequence analysis) and MLST (Multilocus sequence typing) based in the analysis of several housekeeping genes have been applied phylogenetic analyses of concrete groups of rhizobia as Ensifer [63][64][65] and Bradyrhizobium. 66,67 For instance, these studies indicate that housekeeping genes sequencing is superior to DNA-DNA hybridization for the assessment of genetic relatedness between Ensifer species and support the suggestion that Ensifer xinjiangensis is a later heterotypic synonym of Ensifer fredii.…”

Section: Recent Changes In the Taxonomy Of Rhizobiamentioning

confidence: 99%

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Taxonomy of Bacteria Nodulating Legumes

2009

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Over the years, the term "rhizobia" has come to be used for all the bacteria that are capable of nodulation and nitrogen fixation in association with legumes but the taxonomy of rhizobia has changed considerably over the last 30 year. Recently, several nonrhizobial species belonging to alpha and beta subgroup of Proteobacteria have been identified as nitrogen-fixing legume symbionts. Here we provide an overview of the history of the rhizobia and the widespread phylogenetic diversity of nitrogen-fixing legume symbionts.

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Section: Some Problems In Rhizobial Taxonomymentioning

confidence: 84%

Section: Recent Changes In the Taxonomy Of Rhizobiamentioning

confidence: 99%

Section: Recent Changes In the Taxonomy Of Rhizobiamentioning

confidence: 99%

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Taxonomy of Bacteria Nodulating Legumes

2009

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“…The phenotypic characterization was performed in this study using the methodology previously reported [19,23]. The type strains of the type species for all the defined genera in Family Rhizobiaceae were included in the phenotypic study as reference (Table 3).…”

mentioning

confidence: 99%

Pseudorhizobium pelagicum gen. nov., sp. nov. isolated from a pelagic Mediterranean zone

Kimes

López‐Pérez

Flores-Félix

et al. 2015

Systematic and Applied Microbiology

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“…The highest sequence similarities were 87.8 % for recA, 92.2 % for atpD and 89.0 % for glnII to Rhizobium leguminosarum ATCC 14482, Rhizobium tropici USDA 9030 and Rhizobium etli CFN 42 T , respectively. These three most closely related species are all able to nodulate common bean (Martínez-Romero et al, 1991;Ramírez-Bahena et al, 2008;Segovia et al, 1993) in both tropical and temperate regions.…”

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confidence: 99%

Rhizobium tibeticum sp. nov., a symbiotic bacterium isolated from Trigonella archiducis-nicolai (Sirj.) Vassilcz.

Hou

Wang

Liu

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Rhizobium tibeticum sp. nov., a symbiotic bacterium isolated from Trigonella archiducisnicolai (Š irj.) Vassilcz. Isolated from root nodules of Trigonella archiducis-nicolai (Š irj.) Vassilcz. grown in Tibet, China, cells of the bacterial strains CCBAU 85039 T and CCBAU 85027 were Gram-negative, aerobic, motile, non-spore-forming rods that formed colonies that were semi-translucent and opalescent on yeast extract-mannitol agar. In numerical taxonomy, SDS-PAGE analysis of whole-cell proteins and DNA-DNA hybridization, the two strains were very similar and were different from reference strains of defined Rhizobium species. In the phylogeny based on 16S rRNA gene sequences, they were most similar to Rhizobium etli CFN 42 T (98.2 % similarity) and R. leguminosarum USDA 2370 T (97.6 %). Sequence analyses of the housekeeping genes recA, atpD and glnII and the 16S-23S rRNA intergenic spacer, phenotypic characteristics and cellular fatty acid profiles strongly suggested that these two strains represented a novel species within Rhizobium. Crossnodulation tests and sequencing of nifH and nodA genes showed that these two strains were symbiotic bacteria that nodulated Trigonella archiducis-nicolai, Medicago lupulina, Medicago sativa, Melilotus officinalis, Phaseolus vulgaris and Trigonella foenum-graecum. Based on the results, the novel species Rhizobium tibeticum sp. nov. is described to accommodate the two strains. The type strain is CCBAU 85039 T (5LMG 24453 T 5CGMCC 1.7071 T ). The DNA G+C content of this strain is 59.7 mol% (T m ).The genus Rhizobium was first described by Frank (1889) to accommodate all symbiotic nitrogen-fixing bacteria associated with legumes. After a series of revisions, some earlier described species have been moved into Bradyrhizobium (Jordan, 1982), Sinorhizobium (de Lajudie et al., 1994) and Mesorhizobium (Jarvis et al., 1997), while many novel species and genera of rhizobia within the Alphaproteobacteria and Betaproteobacteria have been reported (NZ Rhizobia, 2008). The current genus Rhizobium includes about 30 species of fast-growing, acid-producing rhizobia, which can be differentiated from Sinorhizobium species on the basis of 16S rRNA gene sequence-based phylogeny. With the exception of Rhizobium cellulosilyticus (Garcia-Fraile et al., 2007), most of them can induce root nodules on a certain range of legume species.In a survey of rhizobial resources in Tibet, 12 strains of root-nodule bacteria were isolated with yeast mannitol agar (YMA) medium according to the method of Vincent (1970) from root nodules of Trigonella archiducis-nicolai (Širj.) Vassilcz., an ephemeral legume growing in grasslands at altitudes of 3700-4000 m. Six of them were identified as Sinorhizobium meliloti, and six as Rhizobium strains (Hou et al., 2009). Among the Rhizobium strains, CCBAU 85039 T and CCBAU 85027 were classified as a small group by numerical taxonomy, amplified 16S rRNA gene restriction analysis, amplified 16S-23S intergenic spacer restriction analysis and 16S rRNA gene phylogeny (Hou et al., 2009). To...

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Cited by 155 publications

References 16 publications

Taxonomy of Bacteria Nodulating Legumes

Taxonomy of Bacteria Nodulating Legumes

Pseudorhizobium pelagicum gen. nov., sp. nov. isolated from a pelagic Mediterranean zone

Rhizobium tibeticum sp. nov., a symbiotic bacterium isolated from Trigonella archiducis-nicolai (Sirj.) Vassilcz.

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