Rewarming from accidental hypothermia enhances whole blood clotting properties in a murine model

Enhanced Blood Clotting After Rewarming From Experimental Hypothermia in an Intact Porcine Model

“…Some investigators report the occurrence of a hypothermiainduced coagulopathy, which is completely reversible upon rewarming (Michelson et al, 1994;Valeri et al, 1987). Another study reported enhanced clotting capabilities after rewarming, resulting in thrombus formation in microvessels (Horioka et al, 2020). The aim of the present study was to investigate the impact of hypothermia and subsequent rewarming on whole blood coagulation using an intact pig model of immersion cooling and rewarming.…”

“…In the normothermic control group, all parameters remained unchanged during 6 h of stable normothermia. of activated platelets after rewarming from hypothermia in an in vivo murine model (Horioka et al, 2020). In addition, it has been observed that refrigerated platelets become more hemostatically active upon transfusion, compared to platelets stored at room temperature (Pidcoke et al, 2014).…”

“…Despite observations that hypothermia leads to an increased tendency of bleeding, several studies have reported increased platelet activation and aggregation at lower temperatures (Elis et al, 2002;Hall et al, 2005;Kander et al, 2014;Lindenblatt et al, 2005;Scharbert et al, 2010;Straub et al, 2006Straub et al, , 2011Xavier et al, 2007). Horioka et al found that hypothermia induced activation of platelets in the splenic pool, followed by increased levels of activated platelets in peripheral blood upon rewarming (Horioka et al, 2020(Horioka et al, , 2019. Interestingly, levels of activated platelets in peripheral blood were unchanged in asplenic mice (Horioka et al, 2020).…”

“…Studies using thromboelastography, and also other viscoelastic hemostatic assays, including rotational thrombelastometry and free oscillation rheometry, all report a progressive increase in clotting time at reduced body temperatures (Rundgren and Engström, 2008;Ruzicka et al, 2012;Wallner et al, 2020;Winstedt et al, 2013). These whole blood hemostatic tests measure the dynamics of clot formation and seem to reflect in vivo blood clotting more accurately (Horioka et al, 2020).…”

Alteration in Thrombogenic Parameters as a Potential Ramification of High Altitude Influence on Individuals

“…Prostacyclin's effect on platelet aggregation and endothelial synthesis may be impaired by hypothermia, leading to increased platelet activation and thrombosis. [42,43] According to de Vrij et al, thrombocytopenia during hibernation is primarily caused by body temperature. Mice in vitro research shows that the spontaneous production of P-selectin and the active conformation of GPIIb-IIIa are not significantly altered by incubation at temperatures of 34°C and 31°C.…”