Rex1 (Zfp42) null mice show impaired testicular function, abnormal testis morphology, and aberrant gene expression

Rezende, Naira C.; Lee, Mi-Young; Monette, Sébastien; Mark, Willie; Lu, Ailan; Gudas, Lorraine J.

doi:10.1016/j.ydbio.2011.05.664

Cited by 16 publications

(16 citation statements)

References 58 publications

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“…Following heterozygous intercrosses, homozygous mice were healthy and fertile, although slightly under-represented (). These results confirm previous reports that Rex1 is dispensable for development (Kim et al, 2011; Masui et al, 2008; Rezende et al, 2011). We could derive wild-type, heterozygous and homozygous ES cells, both male and female, from intercross embryos (), demonstrating that Rex1 is not significant for ES cell propagation.…”

Section: Resultssupporting

confidence: 93%

Tracking the embryonic stem cell transition from ground state pluripotency

et al. 2017

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Mouse embryonic stem (ES) cells are locked into self-renewal by shielding from inductive cues. Release from this ground state in minimal conditions offers a system for delineating developmental progression from naïve pluripotency. Here, we examine the initial transition process. The ES cell population behaves asynchronously. We therefore exploited a short-half-life Rex1::GFP reporter to isolate cells either side of exit from naïve status. Extinction of ES cell identity in single cells is acute. It occurs only after near-complete elimination of naïve pluripotency factors, but precedes appearance of lineage specification markers. Cells newly departed from the ES cell state display features of early post-implantation epiblast and are distinct from primed epiblast. They also exhibit a genome-wide increase in DNA methylation, intermediate between early and late epiblast. These findings are consistent with the proposition that naïve cells transition to a distinct formative phase of pluripotency preparatory to lineage priming.

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Section: Resultssupporting

confidence: 93%

Tracking the embryonic stem cell transition from ground state pluripotency

et al. 2017

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“…Rex1 dosage is more critical during spermatogenesis than during oogenesis [34]. This result is consistent with the fact that Rex1 expression is mainly detected in spermatogenesis [23,35].…”

supporting

confidence: 87%

“…In several mouse models, homozygous Rex1-deficient offspring are viable and fertile, although weak phenotypes are observed. Rex1 phenotypes comprise the birth of a reduced number of Rex1-deficient individuals [32], reduced litter size proportional to the extent of Rex1 deficiency [34], and testicular germ cell defects [35]. Important functions of Rex1 during preimplantation development may be compensated for by Yy1 and Yy2, genes whose zinc fingers are related to Rex1.…”

Section: Discussionmentioning

confidence: 99%

Functional Analysis ofRex1During Preimplantation Development

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Alonso-Martín

Pérez-Palacios

et al. 2013

Stem Cells and Development

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Rex1/Zfp42 is a nuclear protein that is highly conserved in mammals, and widely used as an embryonic stem (ES) cell marker. Although Rex1 expression is associated with enhanced pluripotency, loss-of-function models recently described do not exhibit major phenotypes, and both preimplantation development and ES cell derivation appear normal in the absence of Rex1. To better understand the functional role of Rex1, we examined the expression and localization of Rex1 during preimplantation development. Our studies indicated that REX1 is expressed at all stages during mouse preimplantation development, with a mixed pattern of nuclear, perinuclear, and cytoplasmic localization. Chromatin association seemed to be altered in 8-cell embryos, and in the blastocyst, we found REX1 localized almost exclusively in the nucleus. A functional role for Rex1 in vivo was assessed by gain-and loss-of-function approaches. Embryos with attenuated levels of Rex1 after injection of zygotes with siRNAs did not exhibit defects in preimplantation development in vitro. In contrast, overexpression of Rex1 interfered with cleavage divisions and with proper blastocyst development, although we failed to detect alterations in the expression of lineage and pluripotency markers. Rex1 gain-and loss-of-function did alter the expression levels of Zscan4, an important regulator of preimplantation development and pluripotency. Our results suggest that Rex1 plays a role during preimplantation development. They are compatible with a role for Rex1 during acquisition of pluripotency in the blastocyst.

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“…Other studies have demonstrated that Sox9 is present in Sertoli cells at various ages. In addition to being expressed during fetal development [7,8], Sox9 is also expressed in mouse Sertoli cells at P0, P7, P14, P21, P42, P56, P84, and P112 [66][67][68][69][70]. Although none of these studies mention the presence of Sox9 in Leydig cells, current findings suggest that Sox9 could also be expressed in Leydig cells.…”

Section: Discussionmentioning

confidence: 54%

Expressions of Sox9, Sox5, and Sox13 transcription factors in mice testis during postnatal development

2015

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SRY-related box (Sox) transcription factors are conserved among vertebrate species. These proteins regulate multiple processes including sex determination and testis differentiation of the male embryo. Although members of the Sox family have been identified in pre- and postnatal Sertoli cells, they have never been characterized in adult Leydig cells. The objectives of this research were to identify expressions of Sox9, Sox5, and Sox13 in mice Leydig cell cultures and to establish their expression profiles in postnatal mice testes at different developmental stages. Methods used include Western blots and qPCR of stimulated MA-10 cell cultures and whole mice testes. Sox9, Sox5, and Sox13 proteins were detected in MA-10 cells as well as whole mouse testis. Although Sox9, Sox5, and Sox13 mRNA levels from whole mice testes tended to increase according to postnatal development, these results were not significant. Sox members were also detected in whole mice testis by Western Blot. However, Sox9, Sox5, and Sox13 protein expressions remained relatively constant during postnatal development from postnatal (P) day 60 to P365. Being newly characterized in the mouse testis, Sox13 was mainly localized by immunofluorescence within the nuclei of cells from seminiferous tubules, possibly spermatocytes and Sertoli cells. In addition, Sox9, Sox5, and Sox13 proteins were characterized in the nuclei of MA-10 Leydig cell cultures. Their expressions and transcriptional activities remained unaffected by activators of the cAMP/PKA pathway. Thus, Sox9, Sox5, and Sox13 transcription factors are expressed in postnatal testis and may regulate multiple functions such as steroidogenesis and spermatogenesis.

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Rex1 (Zfp42) null mice show impaired testicular function, abnormal testis morphology, and aberrant gene expression

Cited by 16 publications

References 58 publications

Tracking the embryonic stem cell transition from ground state pluripotency

Tracking the embryonic stem cell transition from ground state pluripotency

Functional Analysis ofRex1During Preimplantation Development

Expressions of Sox9, Sox5, and Sox13 transcription factors in mice testis during postnatal development

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