RFLP genetic linkage maps from four F2.3 populations and a joinmap of Gossypium hirsutum L.

Abstract

An RFLP genetic linkage joinmap was constructed from four different mapping populations of cotton ( Gossypium hirsutum L.). Genetic maps from two of the four populations have been previously reported. The third genetic map was constructed from 199 bulk-sampled plots of an F(2.3) (HQ95-6x'MD51ne') population. The map comprises 83 loci mapped to 24 linkage groups with an average distance between markers of 10.0 centiMorgan (cM), covering 830.1 cM or approximately 18% of the genome. The fourth genetic map was developed from 155 bulk-sampled plots of an F(2.3) (119- 5 sub-okrax'MD51ne') population. This map comprises 56 loci mapped to 16 linkage groups with an average distance between markers of 9.3 cM, covering 520.4 cM or approximately 11% of the cotton genome. A core of 104 cDNA probes was shared between populations, yielding 111 RFLP loci. The constructed genetic linkage joinmap from the above four populations comprises 284 loci mapped to 47 linkage groups with the average distance between markers of 5.3 cM, covering 1,502.6 cM or approximately 31% of the total recombinational length of the cotton genome. The linkage groups contained from 2 to 54 loci each and ranged in distance from 1.0 to 142.6 cM. The joinmap provided further knowledge of competitive chromosome arrangement, parental relationships, gene order, and increased the potential to map genes for the improvement of the cotton crop. This is the first genetic linkage joinmap assembled in G. hirsutum with a core of RFLP markers assayed on different genetic backgrounds of cotton populations (Acala, Delta, and Texas plain). Research is ongoing for the identification of quantitative trait loci for agronomic, physiological and fiber quality traits on these maps, and the identification of RFLP loci lineage for G. hirsutum from its diploid progenitors (the A and D genomes).

A Microsatellite-Based, Gene-Rich Linkage Map Reveals Genome Structure, Function and Evolution in Gossypium

“…To 1 These authors contributed equally to this work. 2 date, several genetic maps of cotton genomes have been constructed using diverse DNA molecular markers and mapping populations (Ulloa and Meredith 2000;Ulloa et al 2002;Zhang et al 2002;Lacape et al 2003;Mei et al 2004;Rong et al 2004;Zhang et al 2005;Frelichowski et al 2006). The most saturated tetraploid cotton map is from Rong et al (2004), which is composed of 2584 loci at 1.72-cM intervals in 26 linkage groups.…”

QTL mapping of some productivity and fibre traits in Gossypium arboreum

“…Skewed segregation ratios have been reported frequently in cotton (Ulloa et al, 2002Lacape et al, 2003;Mei et al, 2004;Desai et al, 2006).…”

A Review of QTL Mapping in Cotton: Molecular Markers, Mapping Populations and Statistical Methods

“…In various species of cotton, RFLP markers have been used to study the genetic diversity, population genetics, evolution and phylogenetic relationships (Yu et al, 1997). Brubaker et al, 1999;Meredith et al, 2000 andUlloa et al, 2002 are published genetic mapping of cotton using RFLPs and it was reported that in cotton 64 % RFLPs are codominant in nature (Reinisch et al, 1994). Genetic diversity in upland cotton has also been examined using RFLP markers (Brubaker and wendel et al, 1994).…”