Rg3-loaded P407/CS/HA hydrogel inhibits UVB-induced oxidative stress, inflammation and apoptosis in HaCaT cells

Ding, Chuanbo; Peng, Xiaojuan; Yang, Jiali; Chen, Kecheng; Liu, Xinglong; Zhao, Yingchun; Zhang, Shuai; Sun, Shuwen; Zhang, Jinping; Ding, Qiteng; Liu, S.; Liu, Wencong

doi:10.1016/j.biopha.2023.115177

Cited by 9 publications

(3 citation statements)

References 46 publications

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“…So, the results of TUNEL staining indicate that the group treated with HSE-PPcRu+UVB shows weak positive signals (Figure D,I and Figure S42). In the process of skin inflammation caused by UVB, Cox-2 has been reported to be a critical mediator . Thus, we stain Cox-2 to study its expression levels in skin tissues from HSE-PPcRu-treated mice.…”

Section: Results and Discussionmentioning

confidence: 99%

“…In the process of skin inflammation caused by UVB, Cox-2 has been reported to be a critical mediator. 65 Thus, we stain Cox-2 to study its expression levels in skin tissues from HSE-PPcRu-treated mice. As shown in Figure 7E,J and Figure S43, upon UVB exposure, increased expression of Cox-2 is visualized, while increased expression of Cox-2 by UVB is inhibited in the presence of HSE-PPcRu.…”

Section: Hse-ppcru Protection Of Mouse Skin From Solar Dermatitis In ...mentioning

confidence: 99%

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Conjugated Network Supporting Highly Surface-Exposed Ru Site-Based Artificial Antioxidase for Efficiently Modulating Microenvironment and Alleviating Solar Dermatitis

Yang,

Yuan,

Huang

et al. 2024

ACS Nano

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Solar dermatitis, a form of acute radiation burn that affects the skin, results from overexposure to ultraviolet B (UVB) radiation in strong sunlight. Cell damage caused by the accumulation of reactive oxygen species (ROS) produced by UVB radiation plays an important role in UVBinduced inflammation in the skin. Here, for efficiently scavenging excess ROS, modulating the microenvironment, and alleviating solar dermatitis, a π-conjugated network polyphthalocyanine supporting a highly surfaceexposed Ru active site-based artificial antioxidase (HSE-PPcRu) is designed and fabricated with excellent ROS-scavenging, antioxidant, and antiinflammatory capabilities. In photodamaged human keratinocyte cells, HSE-PPcRu could modulate mitogen-activated protein kinase (MAPK) and nuclear factor kappa-B signaling pathways, prevent DNA damage, suppress apoptosis, inhibit pro-inflammatory cytokine secretion, and alleviate cell damage. In vivo animal experiments reveal the higher antioxidant and antiinflammatory efficacies of HSE-PPcRu by reversing the activation of p38 and c-Jun N-terminal kinase, inhibiting expression of cyclooxygenase-2, interleukin-6, interleukin-8, and tumor necrosis factor-α. This work not only provides an idea for alleviating solar dermatitis via catalytically scavenging ROS and modulating the microenvironment but also offers a strategy to design an intelligent conjugated network-based artificial antioxidase with a highly surface-exposed active site.

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Section: Results and Discussionmentioning

confidence: 99%

Section: Hse-ppcru Protection Of Mouse Skin From Solar Dermatitis In ...mentioning

confidence: 99%

Conjugated Network Supporting Highly Surface-Exposed Ru Site-Based Artificial Antioxidase for Efficiently Modulating Microenvironment and Alleviating Solar Dermatitis

Yang,

Yuan,

Huang

et al. 2024

ACS Nano

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“…We slightly modified the UVB modeling method ( Ding et al, 2023 ). Briefly, we used UVB lamps (PL-S 9W; Royal Dutch Philips Electronics Ltd., Holland, wavelength range: 300–320 nm; peak value: 311 nm) and a UV intensity detector (TM213; Tenmars Electronics Ltd., Taiwan).…”

Section: Methodsmentioning

confidence: 99%

Isolation and purification of Eleutherococcus sessiliflorus (Rupr. & Maxim.) S. Y. Hu peptides and study of their antioxidant effects and mechanisms

Liu,

Ding,

Xie

et al. 2024

Front. Pharmacol.

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Oxidative stress is a state of imbalance between oxidant and antioxidant effects in the body, which is closely associated with aging and many diseases. Therefore, the development of antioxidants has become urgent. In this study, we isolated three polypeptides, G-6-Y, P-8-R, and F-10-W, from Eleutherococcus sessiliflorus (Rupr. & Maxim.) S. Y. Hu (E. sessiliflorus), based on the antioxidant and anti-aging properties of Eleutherococcus, and screened the most powerful free radical scavenging peptide P-8-R. Ultraviolet B (UVB)-induced oxidative stress damage in the skin was established to test the efficacy of P-8-R. In cellular experiments, P-8-R not only prevented oxidative stress damage in HaCaT cells, reduced intracellular reactive oxygen species levels, and inhibited the overexpression of matrix metalloproteinases but also inhibited apoptosis via the mitochondria-dependent apoptotic pathway; in animal experiments, P-8-R was able to prevent oxidative stress damage in the skin and reduce skin collagen loss by inhibiting the overexpression of MMPs to prevent mouse skin aging. In conclusion, the present study contributes to an in-depth understanding of the active compounds of Eleutherococcus, which is of great significance for the pharmacodynamic mechanism and industrial development of Eleutherococcus, and P-8-R is likely to become a potential antioxidant and anti-aging drug or skin care cosmetic in the future.

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Molecular mechanisms behind the inhibitory effects of ginsenoside Rg3 on hepatic fibrosis: a review

Tian,

Hu,

Wang

et al. 2024

Arch Toxicol

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Rg3-loaded P407/CS/HA hydrogel inhibits UVB-induced oxidative stress, inflammation and apoptosis in HaCaT cells

Cited by 9 publications

References 46 publications

Conjugated Network Supporting Highly Surface-Exposed Ru Site-Based Artificial Antioxidase for Efficiently Modulating Microenvironment and Alleviating Solar Dermatitis

Conjugated Network Supporting Highly Surface-Exposed Ru Site-Based Artificial Antioxidase for Efficiently Modulating Microenvironment and Alleviating Solar Dermatitis

Isolation and purification of Eleutherococcus sessiliflorus (Rupr. & Maxim.) S. Y. Hu peptides and study of their antioxidant effects and mechanisms

Molecular mechanisms behind the inhibitory effects of ginsenoside Rg3 on hepatic fibrosis: a review

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