RGD-mediated membrane-matrix adhesion triggers agarose-induced embryoid formation in sunflower protoplasts

Barthou, Henri; Brière, Christian; Souvré, A.; Alibert, Gilbert

doi:10.1007/bf01279261

Cited by 38 publications

(25 citation statements)

References 35 publications

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“…Integrins are also chosen by pathogens as targets for binding, and the RGD motif is present in surface proteins of several pathogens that attach to mammalian cells [5]. In plants, synthetic peptides containing the RGD motif can disrupt the adhesion between plasma membrane and cell wall [6][7] and can interfere with physiological processes thereby affecting, e.g., development [8][9][10][11], mechano-perception [12] and the interaction with microorganisms [13][14][15]. In particular, expression of cell wall associated defence responses triggered by fungal pathogens is dependent on adhesion between the plasma membrane and the plant cell wall [15].…”

Section: Introductionmentioning

confidence: 99%

High affinity recognition of a Phytophthora protein by Arabidopsis via an RGD motif

Senchou

Weide

Carrasco

et al. 2004

Cellular and Molecular Life Sciences (CMLS)

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Section: Introductionmentioning

confidence: 99%

High affinity recognition of a Phytophthora protein by Arabidopsis via an RGD motif

Senchou

Weide

Carrasco

et al. 2004

Cellular and Molecular Life Sciences (CMLS)

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“…Although early efforts to find functional homologs of integrins in plants proved difficult, the recent demonstration of highaffinity RGD binding sites in the plasma membrane of Arabidopsis (Canut et al, 1998), the recent cloning of a gene from Arabidopsis with partial sequence similarity to integrins from fungi, insects, and humans (Nagpal and Quatrano, 1999), and the discovery of Arabidopsis genes that are predicted to contain ␤ -integrin-like domains (Laval et al, 1999) all suggest that there may be at least some conservation between proteins involved in plasma membrane-ECM interactions in plant and animal systems. Further evidence of similarity between these systems is provided by the fact that treatment with RGD-containing peptides can influence several physiological events in plants (Schindler et al, 1989;Wayne et al, 1992;Barthou et al, 1999). RGD peptides also have been shown to cause a loss of plasma membrane-cell wall adhesion in plasmolyzed Arabidopsis cells and a loss of the thin plasma membrane-cell wall connections known as Hechtian strands that form during onion cell plasmolysis (Canut et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Plasma Membrane–Cell Wall Adhesion Is Required for Expression of Plant Defense Responses during Fungal Penetration

2001

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Fungal pathogens almost invariably trigger cell wall-associated defense responses, such as extracellular hydrogen peroxide generation and callose deposition, when they attempt to penetrate either resistant or susceptible plant cells. In the current study, we provide evidence that the expression of these defenses is dependent on adhesion between the plant cell wall and the plasma membrane. Peptides containing an Arg-Gly-Asp (RGD) motif, which interfered with plasma membrane-cell wall adhesion as shown by the loss of the thin plasma membrane-cell wall connections known as Hechtian strands, reduced the expression of cell wall-associated defense responses during the penetration of nonhost plants by biotrophic fungal pathogens. This reduction was associated with increased fungal penetration efficiency. Neither of these effects was seen after treatment with similar peptides lacking the RGD motif. Disruption of plant microfilaments had no effect on Hechtian strands but mimicked the effect of RGD peptides on wall defenses, suggesting that the expression of cell wall-associated defenses involves communication between the plant cell wall and the cytosol across the plasma membrane. To visualize the state of the plasma membrane-cell wall interaction during fungal penetration, we observed living cells during sucrose-induced plasmolysis. In interactions that were characterized by the early expression of cell wall-associated defenses, there was no change, or an increase, in plasma membrane-cell wall adhesion under the penetration point as the fungus grew through the plant cell wall. In contrast, for rust fungus interactions with host plants, there was a strong correlation between a lack of cell wall-associated defenses and a localized decrease in plasma membrane-cell wall adhesion under the penetration point. Abolition of this localized decreased adhesion by previous inoculation with a fungus that increased plasma membrane-cell wall adhesion resulted in reduced penetration by the rust fungus and induction of cell wall-associated defenses. These results suggest that rust fungi may induce a decrease in plasma membrane-cell wall adhesion as a means of disrupting the expression of nonspecific defense responses during penetration of host cells.

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“…This was the reason why this study was undertaken to assess the effects of haemoglobin. Previously it was demonstrated that immobilization of sunflower protoplasts in agarose can also stimulate the development of embryo-like structures (Alibert 1997;Barthou et al 1998). It might be concluded that better oxygenation of sunflower protoplasts in culture does increase total number of embryo-like structures but it is not a key factor for further embryo development.…”

Section: Resultsmentioning

confidence: 98%

In vitro morphogenesis of sunflower (Helianthus annuus) hypocotyl protoplasts: the effects of protoplast density, haemoglobin and spermidine

Rákosy-Tican

Aurori

Vesa

et al. 2007

Plant Cell Tiss Organ Cult

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Sunflower, as one of the most important oil-producing crops, represents an important target for genetic improvement through gene transfer or somatic hybridization. Unfortunately, sunflower is recognized as recalcitrant to in vitro culture. The aim of our paper was to improve sunflower protoplast regeneration. Three cultivars (Romanian hybrids) and one inbred line were used for protoplast isolation from etiolated hypocotyls. Isolated protoplasts were embedded in alginate disks and cultured in two plating densities, using two culture regimes as indicated by previous authors. Plating efficiency, callus development and plant regeneration were evaluated as well as old callus histology. In cv. 'Select', the effects of 1:50 haemoglobin and 1 mM spermidine were assayed on asymmetric division and/ or plating efficiency. Plant regeneration from hypocotyl protoplasts was achieved for two cvs., 'Florom 328' and 'Turbo', with the former proving once more its totipotency. The best culture regime proved to be as recommended by Krasnyanski and Menczel (1993), but the best density in the culture medium was the highest ever tested, 8 · 10 5 pp ml À1 . Moreover, the histology of old green compact protoplast-derived callus revealed a very well organized structure suggesting senescence. In the non-responsive cv. 'Select', haemoglobin was found to stimulate protoplast asymmetric division and the development of heart-shaped embryo-like structures, while spermidine stimulated overall protoplast plating efficiency.

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RGD-mediated membrane-matrix adhesion triggers agarose-induced embryoid formation in sunflower protoplasts

Cited by 38 publications

References 35 publications

High affinity recognition of a Phytophthora protein by Arabidopsis via an RGD motif

High affinity recognition of a Phytophthora protein by Arabidopsis via an RGD motif

Plasma Membrane–Cell Wall Adhesion Is Required for Expression of Plant Defense Responses during Fungal Penetration

In vitro morphogenesis of sunflower (Helianthus annuus) hypocotyl protoplasts: the effects of protoplast density, haemoglobin and spermidine

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