RGD peptide-immobilized electrospun matrix of polyurethane for enhanced endothelial cell affinity

Choi, Won Sup; Bae, Jin Woo; Lim, Hye Ryeon; Joung, Yoon Ki; Park, Jong Chul; Kwon, Il Keun; Парк, Ки Донг

doi:10.1088/1748-6041/3/4/044104

Cited by 59 publications

(28 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, numerous studies have addressed this deficiency via several types of PU surface modification. [40][41][42][43][44][45][46]. PU has been treated with gas plasma to improve EC adhesion and growth [42], although such a treatment also risks increasing the non-specific attachment of undesirable proteins and cells.…”

Section: Discussionmentioning

confidence: 99%

Regulation of polyurethane hemocompatibility and endothelialization by tethered hyaluronic acid oligosaccharides

2009

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Regulation of polyurethane hemocompatibility and endothelialization by tethered hyaluronic acid oligosaccharides

2009

View full text Add to dashboard Cite

“…The crosslinked electrospun PU/PEGMA scaffolds demonstrated mechanical behavior like that of rubber, in that stress-strain curves showed linear elasticity as their intrinsic material property. Generally, the mechanical properties of the electrospun scaffolds depended on the inherent material character and geometric arrangement, which contributed to the most important adhered and tangled effect of fibers [26]. Hence, PEGMA formed crosslinked netlike inner fiber structure of PU through photopolymerization also enhanced the action of fibers through adhered each other.…”

Section: Mechanical Propertiesmentioning

confidence: 99%

Fabrication of PU/PEGMA crosslinked hybrid scaffolds by in situ UV photopolymerization favoring human endothelial cells growth for vascular tissue engineering

Wang

Feng

et al. 2012

J Mater Sci: Mater Med

View full text Add to dashboard Cite

Poly(ethylene glycol) methacrylate (PEGMA) was introduced into a polyurethane (PU) solution in order to prepare electrospun scaffold with improving the biocompatibility by electrospinning technology for potential application as small diameter vascular scaffolds. Crosslinked electrospun PU/PEGMA hybrid nanofibers were fabricated by a reactive electrospinning process with N,N'-methylenebisacrylamide as crosslinker and benzophenone as photoinitiator. The photoinduced polymerization and crosslinking reaction took place simultaneously during the electrospinning process. The electrospinning solutions with various weight ratios of PU/PEGMA were successfully electrospun. No significant difference in the scaffold morphology was found by SEM when PEGMA content was <20 wt%. The crosslinked fibrous scaffolds of PU/PEGMA exhibited higher mechanical strength than the pure PU scaffold. The hydrophilicity of scaffolds was controlled by varying the PU/PEGMA weight ratio. The tissue compatibility of electrospun nanofibrous scaffolds were tested using human umbilical vein endothelial cells (HUVECs). Cell morphology and cell proliferation were measured by SEM, fluorescence microscopy and thiazolyl blue assay (MTT) after 1, 3, 7 days of culture. The results indicated that the cell morphology and proliferation on the crosslinked PU/PEGMA scaffolds were better than that on the pure PU scaffold. Furthermore, the appropriate hydrophilic surface with water contact angle in the range of 55-75° was favorable of improvement the HUVECs adhesion and proliferation. Cells seeded on the crosslinked PU/PEGMA (80/20) scaffolds infiltrated into the scaffolds after 7 days of growth. These results indicated the crosslinked electrospun PU/PEGMA nanofibrous scaffolds were potential substitutes for artificial vascular scaffolds.

show abstract

“…Scaffold morphology is controlled by the type of solvent, polymer concentration and phase separation temperature. This technique can be used to fabricate scaffolds from many types of polymers and polymeric composite materials, obtaining highly interconnected pores with tuneable pore size and also combined macro and microporous structures [107,108]. The method enables to produce also nanofibrous networks with porosity of 98% and fibre diameters in the range 50-500 nm [3].…”

Section: Thermally Induced Phase Separationmentioning

confidence: 99%

“…(iii) surface graft polymerization: covalent immobilization of biomolecules has been performed at fibre surface after pre-treatement with plasma or UV radiation [108][109][110][111].…”

Section: Surface Functionalizationmentioning

confidence: 99%

Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

Gualandi

2011

Springer Theses

View full text Add to dashboard Cite

and some lactide copolymers) and of non-commercial polyesters (i.e. poly-ω-pentadecalactone and some of its copolymers) were explored and discussed.Two techniques were employed to fabricate scaffolds: supercritical carbon dioxide (scCO 2 ) foaming and electrospinning (ES). The former is a powerful technology that enables to produce 3D microporous foams by avoiding the use of solvents that can be toxic to mammalian cells. The scCO 2 process, which is II commonly applied to amorphous polymers, was successfully modified to foam a highly crystalline poly(ω-pentadecalactone-co-ε-caprolactone) copolymer and the effect of process parameters on scaffold morphology and thermo-mechanical properties was investigated.In the course of the present research activity, sub-micrometric fibrous nonwoven meshes were produced using ES technology. Electrospun materials are considered highly promising scaffolds because they resemble the 3D organization of native extra cellular matrix. A careful control of process parameters allowed to fabricate defect-free fibres with diameters ranging from hundreds of nanometers to several microns, having either smooth or porous surface. Moreover, versatility of ES technology enabled to produce electrospun scaffolds from different polyesters as well as "composite" non-woven meshes by concomitantly electrospinning different fibres in terms of both fibre morphology and polymer material. The 3D-architecture of the electrospun scaffolds fabricated in this research was controlled in terms of mutual fibre orientation by properly modifying the instrumental apparatus. This aspect is particularly interesting since the micro/nano-architecture of the scaffold is known to affect cell behaviour.Since last generation scaffolds are expected to induce specific cell response, the present research activity also explored the possibility to produce electrospun scaffolds bioactive towards cells. Bio-functionalized substrates were obtained by loading polymer fibres with growth factors (i.e. biomolecules that elicit specific cell behaviour) and it was demonstrated that, despite the high voltages applied during electrospinning, the growth factor retains its biological activity once

show abstract

RGD peptide-immobilized electrospun matrix of polyurethane for enhanced endothelial cell affinity

Cited by 59 publications

References 50 publications

Regulation of polyurethane hemocompatibility and endothelialization by tethered hyaluronic acid oligosaccharides

Regulation of polyurethane hemocompatibility and endothelialization by tethered hyaluronic acid oligosaccharides

Fabrication of PU/PEGMA crosslinked hybrid scaffolds by in situ UV photopolymerization favoring human endothelial cells growth for vascular tissue engineering

Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

Contact Info

Product

Resources

About