Rhamnolipid production byPseudomonas aeruginosa under denitrification: Effects of limiting nutrients and carbon substrates

Chayabutra, Chawala; Wu, Jianyong; Ju, Lu‐Kwang

doi:10.1002/1097-0290(20010105)72:1<25::aid-bit4>3.0.co;2-j

Cited by 136 publications

(97 citation statements)

References 32 publications

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“…Moreover, hexane was also inefficient in cell growth and rhamnolipid production; resulting in a low rhamnolipid yield and cell dry weight of only 0.07±0.02 and 0.2± 0.01 g/L, respectively, probably due to its poor biodegradability (Chayabutra et al, 2001).…”

Section: Effects Of Carbon Sources On Rhamnolipid Productionmentioning

confidence: 99%

Production and characterization of di-rhamnolipid produced by Pseudomonas aeruginosa TMN

Moussa

Mohamed

Samak

2014

Braz. J. Chem. Eng.

109

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-Pseudomonas aeruginosa TMN was used to produce rhamnolipid (RL) from a variety of carbon and nitrogen substrates. The most favorable carbon sources for RL production were glucose and glycerol (both at 40 g/L), giving a RL yield of 0.3 and 0.25 g/L, respectively. Meanwhile, sodium nitrate appeared to be the preferable nitrogen source, resulting in a RL production of 0.34g/L. Rhamnolipid production from P. aeruginosa TMN was affected by temperature, pH and agitation rate, with 37 °C, pH 7 and 200 rpm agitation favorable for rhamnolipid production. Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR) and electro spray ionization-mass spectrometry (ESI-MS) analyses indicated that the purified product contained one type of commonly found rhamnolipid, which is L-rhamnosyl-L-rhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate. The rhamnolipid product can reduce the surface tension of water to 34 mN/m with a critical micelle concentration of nearly 18.75 mg/L and emulsified kerosene by 46%. P. aeruginosa TMN strain is a potential source of rhamnolipid biosurfactant, which could be used for the development of bioremediation processes in the marine environment.

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Section: Effects Of Carbon Sources On Rhamnolipid Productionmentioning

confidence: 99%

Production and characterization of di-rhamnolipid produced by Pseudomonas aeruginosa TMN

Moussa

Mohamed

Samak

2014

Braz. J. Chem. Eng.

109

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“…The emulsion stability was determined after 24 h. The E 24 was calculated by measuring the emulsion layer formed [16] .…”

Section: Foam Forming Activitymentioning

confidence: 99%

Screening Three Strains of Pseudomonas aeruginosa: Prediction of Biosurfactant-Producer Strain

Dehghannoudeh¹,

Moshafi²,

Behravan³

et al. 2009

American J. of Applied Sciences

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Problem statement:The chemical surfactants have some disadvantages; especially, toxicity and no biodegradability. Approach: Biosurfactants were the structurally diverse group of surface-active molecules synthesize by micro-organisms. The microbial surfactants were interesting, because of the biodegradable and have many applications in industry, agriculture, medicine. Results: In the present study, the production of biosurfactant by three strains of Pseudomonas aeruginosa (PTCC 1074, 1310 and 1430) was investigated. The hemolytic and foam forming activity of different strains were studied and consequently, P. aeruginosa PTCC 1074 was selected as the suitable strain. P. aeruginosa PTCC 1074 was grown in the nutrient broth medium and biosurfactant production was evaluated every 24 h by emulsification index and surface tension for the best of production time. After that, in order to get maximum production of biosurfactant, the selected strain was grown with different additives in nutrient broth and the best culture medium was found. The biosurfactant was isolated from the supernatant and its amphipathic structure was confirmed by chemical methods. Conclusion: Biosurfactant produced by Pseudomonas aeruginosa PTCC 1074 would be considered as a suitable surfactant in industries due to its low toxicity.

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“…Rhamnolipid has been known as biosurfactant which is produced by Pseudomonas aeruginosa in fermentation process. It has been known that rhamnolipid has a strong potential to be used in industrial and bioremediation purposes (Chayabutra, et al, 2001). There are two structure of rhamnolipid which are L-Rhamnosyl-Lrhamnosyl-β-hydroxydecanoyl-β-hydroxydecanoate a n dL -r h a mnos yl -β -hy dr ox y de c a noy l -β -hydroxydecanoate.…”

Section: Introductionmentioning

confidence: 97%

Environmental importance of rhamnolipid production from molasses as a carbon source

Rashedi

Assadi

Bonakdarpour

et al. 2005

Int. J. Environ. Sci. Technol.

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Rhamnolipid has been known as biosurfactant which is produced by Pseudomonas aeruginosa in fermentation process. Several carbon sources such as ethanol, glucose, vegetable oil and hydrocarbon have been used to produce rhamnolipid. In this study, we are trying to use molasses which is a waste product from sugar industry as carbon source to produce rhamnolipid. The bacterium which was previously isolated from Iranian oil over years Glycolipid production by isolated bacterium using sugar beet molasses as a carbon and energy source was investigated. Result from the study showed that the growth of the bacteria using molasses as carbon sources is growth-associated. The specific production rate of rhamnolipid with 2%, 4%, 6%, 8% and 10% of molasses are 0. 00065, 4.556, 8.94, 8.85, and 9.09 respectively. The yield of rhamnolipid per biomass with 2%, 4%, 6%, 8% and 10% molasses are 0.003, 0.009, 0.053, 0.041 and 0.213 respectively. The production of rhamnolipid (0.0531 g. rhamnolipid/g biomass) is higher compare to the culture grown in aerobic condition (0.04 g. rhamnolipid/g biomass). These studies indicate that renewable, relatively inexpensive and easily available resources can be used for important biotechnological processes.

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Rhamnolipid production byPseudomonas aeruginosa under denitrification: Effects of limiting nutrients and carbon substrates

Cited by 136 publications

References 32 publications

Production and characterization of di-rhamnolipid produced by Pseudomonas aeruginosa TMN

Production and characterization of di-rhamnolipid produced by Pseudomonas aeruginosa TMN

Screening Three Strains of Pseudomonas aeruginosa: Prediction of Biosurfactant-Producer Strain

Environmental importance of rhamnolipid production from molasses as a carbon source

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