Rhamnolipids as emulsifying agents for essential oil formulations: Antimicrobial effect against Candida albicans and methicillin-resistant Staphylococcus aureus

Haba, E.; Bouhdid, Samira; Torrego-Solana, Noelia; Marqués, Ana; Espuny, M. J.; García-Celma, María José; Manresa, Ángeles

doi:10.1016/j.ijpharm.2014.09.039

Cited by 92 publications

(57 citation statements)

References 56 publications

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“…However, the emulsions used in this study were fabricated using a simple shaking method and contained droplets that were too large for most commercial applications. Other studies have also reported that rhamnolipids can be used to form oil-in-water emulsions from a variety of different types of oils, but again the methods used to prepare the emulsions only involved simple shearing methods that led to the formation of large oil droplets [25][26][27]. One recent study showed that oil-in-water emulsions containing relatively small droplets (d < 700 nm) could be produced from rhamnolipids using a high pressure homogenizer [28] .…”

Section: Introductionmentioning

confidence: 99%

Formation and stabilization of nanoemulsions using biosurfactants: Rhamnolipids

Bai

McClements

2016

Journal of Colloid and Interface Science

211

109

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Nanoemulsions are used in the food, cosmetics, personal care and pharmaceutical industries to provide desirable optical, textural, stability, and delivery characteristics. In many industrial applications, it is desirable to formulate nanoemulsions using natural ingredients so as to develop label-friendly products. Rhamnolipids are biosurfactants isolated from certain microorganisms using fermentation processes. They are glycolipids that have a polar head consisting of rhamnose units and a non-polar tail consisting of a hydrocarbon chain. In this study, the interfacial characteristics of this natural surfactant at medium chain triglyceride (MCT) oil-water interfaces were characterized, and its ability to form nanoemulsions was compared to that of another natural surfactant (quillaja saponins). The influence of rhamnolipid concentration, homogenization pressure, and oil type on the mean droplet diameter of emulsions produced by microfluidization was determined. Rhamnolipids were highly effective at forming small droplets (d32<0.15μm) at low surfactant-to-oil ratios (SOR<1:10) for MCT oil. Rhamnolipids could also be used to form small droplets using long chain triglyceride oils, such as corn and fish oil. Rhamnolipid-coated droplets were stable to aggregation over a range of pH values (5-9), salt concentrations (<100mM NaCl) and temperatures (20-90°C). However, droplet aggregation was observed at highly acidic (pH 2-4) and high ionic strength (200-500mM NaCl) conditions. These effects were attributed to a reduction in electrostatic repulsion at low pH and high salt levels. Rhamnolipid-coated droplets had a high negative charge at neutral pH that decreased in magnitude with decreasing pH. These results indicate that rhamnolipids are effective natural surfactants that may be able to replace synthetic surfactants in certain commercial applications.

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Section: Introductionmentioning

confidence: 99%

Formation and stabilization of nanoemulsions using biosurfactants: Rhamnolipids

Bai

McClements

2016

Journal of Colloid and Interface Science

211

109

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“…The antimicrobial properties of rhamnolipid have often been reported (Gomaa, 2013;Liu et al, 2017;Santos et al, 2016;Varjani & Upasani, 2017). Very recently, defined rhamnolipids have shown good inhibition behavior against either photogenic bacteria (e.g., K. pneumonia, L. monocytogenes, P. aeruginosa, and S. aureus) (de Araujo et al, 2016;Haba et al, 2014) or phytopathogenic fungal species (e.g., C. albicans, Mucor miehei, Neurospora crassa, and Yarrowia lipolytica) (Sharma, Kalita, & Duarah, 2017;Silva et al, 2017).…”

Section: Antimicrobial Activity Of the Sacmentioning

confidence: 99%

Using Corn Husk Powder as a Novel Substrate to Produce a Surface Active Compound from Labrenzia aggregate KP‐5

Saimmai

Maneerat

Chooklin

2018

J Surfact & Detergents

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In this study, surface active compound (SAC)‐producing bacterial isolates were evaluated for SAC production using corn husk powder (CHP) as a sole carbon source. From the 51 isolates screened, Labrenzia aggregate KP‐5 produced the highest SAC activity. The highest SAC production (3.51 g L−1) was obtained when the strain was cultivated in a minimal salt medium containing 40 g L−1 CHP and 1 g L−1 commercial monosodium glutamate at 30 °C and 150 rpm after 51 h of cultivation. The produced SAC had the ability to decrease the surface tension of water from 72.0 to 25.5 mN m−1, with the critical micelle concentration of 9 mg L−1 (11.07 mM) and exhibited the highest emulsification activity (EA) of 81% against motor oil. The SAC showed stability at 4–121 °C and pH 4–10 against the surface and EA of vegetable oils and hydrocarbons, and showed tolerance at high salt concentrations (1–10% NaCl). The chemical structure of the SAC was confirmed as a rhamnolipid using Fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, and mass spectrometric analysis. The SAC did not exhibit inhibitory effects on various vegetables tested; however, strong inhibitory activity against Gram‐positive and Gram‐negative bacteria was observed. The application of SAC for microbial enhanced oil recovery by sand saturated with used lubricating oil resulted in above 89% of oil removal. The properties of the SAC we obtained from CHP have potential applications especially for microbial enhanced oil recovery and/or reducing the intensity of environmental contamination. In addition, the obtained SAC is a suitable alternative to antimicrobial agents.

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“…developed rhamnolipid‐based emulsions containing essential oils of Melaleuca alternifolia , Cinnamomum verum , Origanum compactum and Lavandula angustifolia using the titration method. The surface properties of rhamnolipids contribute to the positive dispersion of essential oils and thus increase their availability and antimicrobial activity against C. albicans and S. aureus …”

Section: Potential Applications Of Glycolipid Biosurfactants In the Fmentioning

confidence: 99%

Glycolipid biosurfactants: main properties and potential applications in agriculture and food industry

2016

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Glycolipids, consisting of a carbohydrate moiety linked to fatty acids, are microbial surface active compounds produced by various microorganisms. They are characterized by high structural diversity and have the ability to decrease the surface and interfacial tension at the surface and interface, respectively. Rhamnolipids, trehalolipids, mannosylerythritol lipids and cellobiose lipids are among the most popular glycolipids. They have received much practical attention as biopesticides for controlling plant diseases and protecting stored products. As a result of their antifungal activity towards phytopathogenic fungi and larvicidal and mosquitocidal potencies, glycolipid biosurfactants permit the preservation of plants and plant crops from pest invasion. Also, as a result of their emulsifying and antibacterial activities, glycolipids have great potential as food additives and food preservatives. Furthermore, the valorization of food byproducts via the production of glycolipid biosurfactant has received much attention because it permits the bioconversion of byproducts on valuable compounds and decreases the cost of production. Generally, the use of glycolipids in many fields requires their retention from fermentation media. Accordingly, different strategies have been developed to extract and purify glycolipids. © 2016 Society of Chemical Industry

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Rhamnolipids as emulsifying agents for essential oil formulations: Antimicrobial effect against Candida albicans and methicillin-resistant Staphylococcus aureus

Cited by 92 publications

References 56 publications

Formation and stabilization of nanoemulsions using biosurfactants: Rhamnolipids

Formation and stabilization of nanoemulsions using biosurfactants: Rhamnolipids

Using Corn Husk Powder as a Novel Substrate to Produce a Surface Active Compound from Labrenzia aggregate KP‐5

Glycolipid biosurfactants: main properties and potential applications in agriculture and food industry

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