Rheological and morphological characterization of Streptomyces olindensis growing in batch and fed-batch fermentations

Abstract

-Mathematical correlations between rheological properties of the fermentation broth (consistency index, K, and flow behavior index, n), biomass concentration (X) and average clump dimension (D) of Streptomyces olindensis in bioreactor cultivations were obtained, during batch and fed-batch processes. Two types of correlations were compared: the first considered the influence of only biomass concentration (X) on rheological properties (K and n), the second considered the influence of both biomass concentration (X) and morphology (average clump dimension, D) on rheological properties. Clump dimension was assessed by image analysis. Clumps were shown to be the major morphological class during all runs. Incorporation of the morphological parameter in the model improved the capacity to predict the experimental values of the consistency index (K).

The role of volumetric power input in the growth, morphology, and production of a recombinant glycoprotein by Streptomyces lividans in shake flasks

“…The pseudoplastic changes may surely be associated with the changes in morphological sizes at late stages of the cultures in NF. Similar behaviors were found for mycelial cultures of S. clavuligerus [43], S. pristinaespiralis [23], and S. olindensis [10,44]. We could not realize whether these rheological changes are due to morphology or composition of the culture medium modifications, but these rheological changes concur with P/V behavior at the end of cultures.…”

Application of Actinobacterial and Fungal Morphology on the Design of Operating Strategies in Bioprocess Development

“…Streptomycetes reproduce and disperse through the formation of spores, called conidia, which follows the period of vegetative growth. The spores are produced in aerial filaments (sporophores), which rise above the colony.The morphology of Streptomyces olindensis was differentiated into four classes: pellets, clumps, branched, and unbranched free filaments by image analysis [7].…”