Rheological characterization of hydrogels formed by recombinantly produced spider silk

Rammensee, Sebastian; Huemmerich, Daniel; Hermanson, Kevin D.; Scheibel, Thomas; Bausch, Andreas R.

doi:10.1007/s00339-005-3431-x

Cited by 147 publications

(134 citation statements)

References 25 publications

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“…Generally, the increase in modulus with crosslinking is in agreement with earlier experiments on hydrogels. 4,5 However, the relative stiffening effect is not as pronounced. A reason for this observation is attributed to the high β-sheet content (64%), already in the non-crosslinked particles.…”

Section: Biomaterials Science Papermentioning

confidence: 98%

“…3,5,7 Especially for eADF4(C16) hydrogels an impact of chemical crosslinking on the rheological behavior of the hydrogels has been observed. 4 In order to determine the effect of crosslinking on the mechanical behavior of the particles, the influence of molecular weight (influencing physical crosslinking) (Fig. 3a) and chemical crosslinking (Fig.…”

Section: Effect Of Molecular Weight and Crosslinking On Particle Propmentioning

confidence: 99%

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Micromechanical characterization of spider silk particles

Neubauer

Blüm

Agostini³

et al. 2013

Biomater. Sci.

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a Spider silk fibers are well known for their mechanical properties, and they are therefore in the focus of materials scientists. Additionally, silks display biocompatibility making them interesting materials for applications in medicine or cosmetics. Due to the low abundance of natural spider silk proteins because of the spider's cannibalism, the recombinant spider silk protein eADF4 has been established for material science applications. Once processed into micron-sized particles by controlled salting-out, these particles can be used as drug delivery vehicles. For any application of the silk particles it is important to know their mechanical characteristics for processing and storage reasons. Here, we examine the swelling behavior and mechanics of these particles. Upon hydration, a drastic drop in elastic modulus occurs by orders of magnitude, from 0.8 GPa in the dry state to 2.99 MPa in the wet state. Importantly, the elastic modulus of recombinant silk particles can be tuned by varying the molecular weight of the used proteins, as well as chemical crosslinking thereof.

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Section: Biomaterials Science Papermentioning

confidence: 98%

Section: Effect Of Molecular Weight and Crosslinking On Particle Propmentioning

confidence: 99%

Micromechanical characterization of spider silk particles

Neubauer

Blüm

Agostini³

et al. 2013

Biomater. Sci.

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“…Producing engineered spider silk proteins recombinantly in bacteria provides, however, new possibilities for the investigation of spinning processes (5)(6)(7). In this work, we used eADF3 and eADF4, previously established engineered variants of the dragline silk fibroins ADF3 and ADF4 of the garden spider Araneus diadematus (8).…”

mentioning

confidence: 99%

Assembly mechanism of recombinant spider silk proteins

Rammensee¹,

Slotta

Scheibel

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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356

377

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Spider silk threads are formed by the irreversible aggregation of silk proteins in a spinning duct with dimensions of only a few micrometers. Here, we present a microfluidic device in which engineered and recombinantly produced spider dragline silk proteins eADF3 (engineered Araneus diadematus fibroin) and eADF4 are assembled into fibers. Our approach allows the direct observation and identification of the essential parameters of dragline silk assembly. Changes in ionic conditions and pH result in aggregation of the two proteins. Assembly of eADF3 fibers was induced only in the presence of an elongational flow component. Strikingly, eADF4 formed fibers only in combination with eADF3. On the basis of these results, we propose a model for dragline silk aggregation and early steps of fiber assembly in the microscopic regime.colloids ͉ microfluidics ͉ protein materials ͉ rheology

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“…Silk fibroin can be processed into various forms such as gels [11], films, fibers [12], powders [13], sponges, and hydrogels [14]. Films of silk fibroin are produced primarily by solution casting methods [8,15].…”

Section: Introductionmentioning

confidence: 99%

Influence of organic solvents on the structural and thermal characteristics of silk protein from the web of Orthaga exvinacea Hampson (Lepidoptera: Pyralidae)

Narayanan

Gokuldas

2016

J Chem Biol

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The silk protein from the web of Orthaga exvinacea was isolated, purified, and casted into films. This film was treated separately with methanol, acetone, ethyl acetate, and isopropyl alcohol in 50 % concentration for about 30 min. The treated films were thus dried in a desiccator and subjected to FTIR and TG-DTA analysis. The structural studies revealed that the organic solvents induce conformatory changes in the protein film, especially the most sensitive amide I (1650 cm −1 ) band. This band had shifted to lower wavenumber (1633-1636 cm −1 ).Furthermore, the conformatory characteristics associated with amide I band also changed from random coil to β-sheet. Generally, β-sheet contributes strength to the protein film. Among the treated films, film treated with acetone showed much thermal stability. Moreover, the film treated with methanol had shown two different temperatures of maximum degradation. It is concluded that in addition to β-sheet content, various other factors such as various processing conditions and structural organization of protein may influence the stability of the films.

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Rheological characterization of hydrogels formed by recombinantly produced spider silk

Cited by 147 publications

References 25 publications

Micromechanical characterization of spider silk particles

Micromechanical characterization of spider silk particles

Assembly mechanism of recombinant spider silk proteins

Influence of organic solvents on the structural and thermal characteristics of silk protein from the web of Orthaga exvinacea Hampson (Lepidoptera: Pyralidae)

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