Rheological characterization of mammalian lung mucus

Vasquez, Erick S.; Bowser, J.E.; Swiderski, Cyprianna E.; Walters, Keisha B.; Kundu, Santanu

doi:10.1039/c4ra05055j

Cited by 23 publications

(26 citation statements)

References 20 publications

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“…They obtained viscosity reduction ratios varying from 1.5 to 7.2 depending on the type of surfactant under physiological shear rates (0.1 to 1 s −1 in the tracheobronchial tree). The shear‐thinning behavior of mammalian lung mucus is also cited by Vasquez et al but not quantified. It is also the case of Boegh et al who tried to propose a bio‐similar mucus composition displaying rheological properties (notably in terms of shear‐thinning) close to the composition of a mucus isolated from cultured cells.…”

Section: Discussionmentioning

confidence: 99%

“…In these cases, the measured properties mostly reflect the flow behavior of mucus and can be used to investigate its viscosity under physiological shearing rates prevailing in human lungs during normal functioning or during temporary events such as cough. Even in studies where both dynamic oscillatory and shear flow measurements were carried out, the thixotropic nature of mucus was not accounted for, or at least not quantitatively . As a consequence, a complete and intrinsically consistent characterization campaign is still missing in the open literature.…”

Section: Introductionmentioning

confidence: 99%

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Rheological properties of synthetic mucus for airway clearance

Lafforgue

Seyssiecq

Poncet

et al. 2017

J Biomedical Materials Res

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In this work, a complete rheological characterization of bronchial mucus simulants based on the composition proposed by Zahm et al. (Eur Respir J 1991; 4:311-315) is presented. Dynamic small amplitude oscillatory shear (SAOS) experiments, steady state (SS) flow measurements and three intervals thixotropy tests (3ITT), are carried out to investigate the global rheological complexities of simulants (viscoelasticity, viscoplasticity, shear-thinning, and thixotropy) as a function of scleroglucan concentrations (0.5-2 wt %) and under temperatures of 20 and 37°C. SAOS measurements show that the limit of the linear viscoelastic range as well as the elasticity both increase with increasing sclerogucan concentrations. Depending on the sollicitation frequency, the 0.5 wt % gel response is either liquid-like or solid-like, whereas more concentrated gels show a solid-like response over the whole frequency range. The temperature dependence of gels response is negligible in the 20-37°C range. The Herschel-Bulkley (HB) model is chosen to fit the SS flow curve of simulants. The evolution of HB parameters versus polymer concentration show that both shear-thinning and viscoplasticity increase with increasing concentrations. 3ITTs allow calculation of recovery thixotropic times after shearings at 100 or 1.6 s . Empiric correlations are proposed to quantify the effect of polymer concentration on rheological parameters of mucus simulants. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 386-396, 2018.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rheological properties of synthetic mucus for airway clearance

Lafforgue

Seyssiecq

Poncet

et al. 2017

J Biomedical Materials Res

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“…In addition to increase in gelation temperature, the gel strength and the complex viscosity of the gel decreased due to incorporation of chitosan (Table 2). Although the viscosity of the formulation was lower than that observed for formulation without chitosan, it was still higher than the nasal mucosal viscosity which ranges between 0.02–500 Pa.s at a frequency of 0.01–100 1/sec [44, 45]. …”

Section: Resultsmentioning

confidence: 99%

Delivery of ziconotide to cerebrospinal fluid via intranasal pathway for the treatment of chronic pain

Manda

Kushwaha

Kundu

et al. 2016

Journal of Controlled Release

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The purpose of the current study was to investigate the plausibility of delivery of ziconotide to the cerebrospinal fluid (CSF) via intranasal administration. Ziconotide was administered either in the form of solution or Kolliphor P 407 gels (KP 407) intranasally in Sprague-Dawley rats. The effect of incorporation of chitosan in the formulation was also investigated. Time course of drug in the CSF was investigated by collecting CSF from cisterna magna. Pharmacokinetics of ziconotide in CSF following intrathecal and intravenous (i.v) administration of ziconotide was investigated. Upon intrathecal administration the elimination rate constant of ziconotide in CSF was found to be 1.01 ± 0.34 h−1. The Cmax and Tmax of ziconotide in CSF following intravenous administration were found to be 37.78 ± 6.8 ng/mL and ~2 h respectively. The time required to attain maximum concentration (Tmax) in CSF was less upon intranasal administration (15 min) compared to i.v administration (120 min). Presence of chitosan enhanced the overall bioavailability of ziconotide from intranasal solution and gel formulations. The elimination rate constant of ziconotide in CSF following intranasal and intravenous administration of ziconotide solution was found to be 0.54 ± 0.08 h−1 and 0.42 ± 0.10 h−1 respectively. Whereas, intranasal administration of ziconotide in the form of in situ forming gel lowered the elimination rate significantly. These results suggest that intranasal administration could be a potential noninvasive and patient compliant method of delivering ziconotide to CSF to treat chronic pain.

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“…The stress hardening of porcine small intestinal mucus reported here is apparent in bulk material behavior in the small deformation regime. This is in contrast to other hardening behaviors reported for mucus which are apparent in the large deformation regime [28,38]. These behaviors have primarily been investigated under oscillatory shear and are evident when one examines the response of the mucus during a single oscillatory cycle by plotting a Lissajous curve, which is a parametric plot of stress versus strain, corresponding to an oscillatory shear test with a sinusoidal stress input at a particular frequency and amplitude [39].…”

Section: Dynamic Responses To Deformationmentioning

confidence: 92%

“…In between these two points, however, there is evidence of non-linearity in the Lissajous plot (Fig. 8b) with a slight upward turn in the stress measure, indicating the small intestinal mucus may also exhibit some of the hardening behaviors identified in the large deformation regime in other mucus secretions [28,38]. It should be noted that this study was undertaken on a sample of mucus from a different pool to the creep tests hence the differences in absolute stress values needed to introduce the same strain.…”

Section: Dynamic Responses To Deformationmentioning

confidence: 92%