Rhesus Antibodies Demonstrable only by Enzyme Technique

Gundolf, F.

doi:10.1111/j.1423-0410.1973.tb02658.x

Cited by 4 publications

(1 citation statement)

References 2 publications

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“…The gel test is more sensitive than the conventional tube method [15, 16]. It has been well known for a long time that the enzyme treatment of RBCs modifies the erythrocyte surface [17–19] and that some Rh antibodies occur only in the enzyme (papain) technique [20, 21]. The main argument for the use of the enzyme technique in the routine testing would be to detect clinically significant RBC alloantibodies, but published works on this topic are rare [22].…”

Section: Introductionmentioning

confidence: 99%

Frequencies and Specificities of “Enzyme-Only” Detected Erythrocyte Alloantibodies in Patients Hospitalized in Austria: Is an Enzyme Test Required for Routine Red Blood Cell Antibody Screening?

Enko

Habres

Wallner

et al. 2014

Journal of Blood Transfusion

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The aim of this study was to determine the frequencies and specificities of “enzyme-only” detected red blood cell (RBC) alloantibodies in the routine antibody screening and antibody identification in patients hospitalized in Austria. Routine blood samples of 2420 patients were investigated. The antibody screening was performed with a 3-cell panel in the low-ionic strength saline- (LISS-) indirect antiglobulin test (IAT) and with an enzyme-pretreated (papain) 3-cell panel fully automated on the ORTHO AutoVue Innova System. The antibody identification was carried out manually with an 11-cell panel in the LISS-IAT and with an enzyme-pretreated (papain) 11-cell panel. In total 4.05% (n = 98) of all patients (n = 2420) had a positive RBC antibody screening result. Of them 25.51% (25/98) showed “enzyme-only” detected specific or nonspecific RBC alloantibodies. Rhesus and Lewis system antibodies were found the only specificities of “enzyme-only” RBC alloantibodies: all in all 4.8% (4/98) were detected with anti-E, 3.06% (3/98) with anti-Lea, 3.06% (3/98) with anti-D after anti-D prophylaxis and 1.02% (1/98) with anti-e. In total, 14.29% (14/98) showed a nonspecific RBC alloantibody result with the enzyme test. The results of the present study demonstrate that a high number of unwanted positive reactions with the enzyme technique overshadows the detection of “enzyme-only” RBC alloantibodies. (Trial Registration: K-37-13).

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Section: Introductionmentioning

confidence: 99%

Frequencies and Specificities of “Enzyme-Only” Detected Erythrocyte Alloantibodies in Patients Hospitalized in Austria: Is an Enzyme Test Required for Routine Red Blood Cell Antibody Screening?

Enko

Habres

Wallner

et al. 2014

Journal of Blood Transfusion

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Ictère hémolytique néonatal par anti-D réagissant préférentiellement en milieu enzymatique

Bizot¹,

Monis²,

Rieu³

1974

Revue Française de Transfusion

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Antenatal Blood Group Serology in Sudanese Women

1978

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2,140 pregnant Sudanese women attending the antenatal clinic in Omdurman Maternity Hospital during the period November 1975 to December 1976 had ABO and Rh typing performed. Their sera were also screened by a modified papain enzyme technique for the presence of atypical antibodies. The rate of Rhesus immunization, the frequency distribution of blood groups, the atypical antibodies encountered, and the advantages of the method used for screening the sera in a developing country are discussed.

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Rhesus Antibodies Demonstrable only by Enzyme Technique

Cited by 4 publications

References 2 publications

Frequencies and Specificities of “Enzyme-Only” Detected Erythrocyte Alloantibodies in Patients Hospitalized in Austria: Is an Enzyme Test Required for Routine Red Blood Cell Antibody Screening?

Frequencies and Specificities of “Enzyme-Only” Detected Erythrocyte Alloantibodies in Patients Hospitalized in Austria: Is an Enzyme Test Required for Routine Red Blood Cell Antibody Screening?

Ictère hémolytique néonatal par anti-D réagissant préférentiellement en milieu enzymatique

Antenatal Blood Group Serology in Sudanese Women

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