Rhinovirus increases human Î²-defensin-2 and -3 mRNA expression in cultured bronchial epithelial cells

Duits, L. A.; Nibbering, Peter H.; Strijen, E. Van; Vos, Joost B.; Mannesse-Lazeroms, S. P. G.; Sterkenburg, M. A. J. A. Van; Hiemstra, Pieter S.

doi:10.1016/s0928-8244(03)00106-8

Cited by 123 publications

(120 citation statements)

References 44 publications

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“…Defensins have been reported in antiviral immune responses in mammalian cells against adenovirus (4), rhinovirus (14), and retrovirus (50,53). We are currently investigating if the defensins detected in this study have any anti-TSWV activity.…”

Section: Discussionmentioning

confidence: 84%

The Plant Virus Tomato Spotted Wilt Tospovirus Activates the Immune System of Its Main Insect Vector, Frankliniella occidentalis

Medeiros¹,

Resende

Ávila

2004

J Virol

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Section: Discussionmentioning

confidence: 84%

The Plant Virus Tomato Spotted Wilt Tospovirus Activates the Immune System of Its Main Insect Vector, Frankliniella occidentalis

Medeiros¹,

Resende

Ávila

2004

J Virol

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“…Here we demonstrate, for the first time to the best of our knowledge, that HBD2 expression is inducible in primary FTEC upon stimulation with poly(I:C). This is significant because HBD2 is an effective antimicrobial against bacterial (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus) as well as viral (adenovirus, HSV, rhinovirus, and HIV) agents (68)(69)(70)(71)(72). In addition, it has chemotactic functions and can facilitate the recruitment of immune cells, such as memory T lymphocytes, iDC and mast cells (39).…”

Section: Discussionmentioning

confidence: 99%

Antiviral responses of human Fallopian tube epithelial cells to toll-like receptor 3 agonist poly(I:C)

Ghosh¹,

Schaefer²,

Fahey³

et al. 2008

Fertility and Sterility

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Objective-To examine the expression of toll-like receptors (TLR) by primary human Fallopian tube epithelial cells (FTEC) and to determine whether exposure to the TLR3 agonist poly(I:C) would induce an antiviral response.Design-Tissue culture study. Setting-University Medical Center. Patient(s)-Pre-menopausal women undergoing hysterectomy.Intervention(s)-Primary human FTEC were grown to confluence and high transepithelial resistance and treated with TLR agonists. Conditioned media was collected and RNA was extracted and analyzed for the expression of cytokines, chemokines and antimicrobial genes.Main Outcome Measure(s)-RNA was analyzed by real-time RT-PCR and protein levels were assessed by ELISA. Result(s)-The FTEC were demonstrated to express TLR1-9 but not 10. Treatment of FTEC with TLR3 agonist poly(I:C) resulted in increased expression of IL-8, TNF-α, human β-defensin 2, interferon beta, and interferon stimulated genes myxovirus resistance gene 1, 2′,5′-oligoadenylate synthetase, and protein kinase R. Additionally, FTEC exposed to poly(I:C) also resulted in the induction of TLR 2, 3, and 7.Conclusion(s)-Our results suggest that FTEC are sensitive to viral infection and/or exposure to viral dsRNA and can respond by secreting proinflammatory cytokines that mediate the initiation of an inflammatory response as well as expressing genes that can directly inhibit viral replication.

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“…[11][12][13] These antimicrobial polypeptides, some of which are induced in response to PAMP exposure, are effective at inhibiting Gram-positive and Gram-negative bacteria, fungi, and enveloped viruses, including Neisseria gonorrhoeae, Chlamydia trachomatis, Candida albicans, Herpes Simplex Virus-2, and Human Immunodeficiency Virus-1. [14][15][16][17][18][19][20][21][22][23] It is well known that estrogens, which act throughout the body, have both anti-inflammatory and proinflammatory functions. For example, E 2 enhances the production of proinflammatory interferon-γ and anti-inflammatory IL-10 in T cells.…”

Section: Introductionmentioning

confidence: 99%

Estradiol selectively regulates innate immune function by polarized human uterine epithelial cells in culture

et al. 2008

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The goal of this study was to examine the role of E 2 in regulating innate immune protection by human uterine epithelial cells (UECs). Recognizing that UECs produce cytokines and chemokines to recruit and activate immune cells as well as viral and bacterial antimicrobials, we sought to examine the effect of E 2 on constitutive and Toll-like receptor (TLR) agonist (lipopolysaccharide (LPS) and poly (I:C))-induced immune responses. The secretion by polarized UECs in culture of interleukin (IL)-6, macrophage inhibitory factor (MIF), and secretory leukocyte protease inhibitor (SLPI) was examined as well as the mRNA expression of human β-defensin-2 (HBD2), tumor necrosis factor (TNF)-α, IL-8, and nuclear factor (NF)-kB. When incubated with E 2 for 24-48 h, we found that E 2 stimulated UEC secretion of SLPI (fourfold) and mRNA expression of HBD2 (fivefold). Moreover, when antibacterial activity in UEC secretions was measured using Staphylococcus aureus, E 2 increased the secretion of soluble factor(s) with antibacterial activity. In contrast, E 2 had no effect on constitutive secretion of proinflammatory cytokines and chemokines by UECs but completely inhibited LPS-and poly (I:C)-induced secretion of MIF, IL-6, and IL-8. Estradiol also reversed the stimulatory effects of IL-1β on mRNA expression of TNF-α, IL-8, and NF-kB by 85, 95, and 70%, respectively. As SLPI is known to inhibit NF-kB expression, these findings suggest that E 2 inhibition of proinflammatory cytokines may be mediated through SLPI regulation of NF-kB. Overall, these findings indicate that the production of cytokines, chemokines, and antimicrobials by UECs are differentially regulated by E 2 . Further, it suggests that with E 2 regulation, epithelial cells that line the uterine cavity have evolved immunologically to be sensitive to viral and bacterial infections as well as the constraints of procreation.

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Rhinovirus increases human Î²-defensin-2 and -3 mRNA expression in cultured bronchial epithelial cells

Cited by 123 publications

References 44 publications

The Plant Virus Tomato Spotted Wilt Tospovirus Activates the Immune System of Its Main Insect Vector, Frankliniella occidentalis

The Plant Virus Tomato Spotted Wilt Tospovirus Activates the Immune System of Its Main Insect Vector, Frankliniella occidentalis

Antiviral responses of human Fallopian tube epithelial cells to toll-like receptor 3 agonist poly(I:C)

Estradiol selectively regulates innate immune function by polarized human uterine epithelial cells in culture

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